P0C1F6 (CCME_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c-type biogenesis protein CcmE

Alternative name(s):
Cytochrome c maturation protein E
Heme chaperone CcmE

Gene names

Name:	ccmE
Synonyms:	cycJ

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH By similarity. HAMAP-Rule MF_01959
Subcellular location	Cell inner membrane; Single-pass type II membrane protein; Periplasmic side Potential HAMAP-Rule MF_01959.
Sequence similarities	Belongs to the CcmE/CycJ family.
Caution	Was originally (Ref.1) proposed to be fused with ccmD.
Sequence caution	The sequence AAC44225.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Gene Ontology (GO)
Biological_process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW protein-heme linkage Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	Cytochrome c-type biogenesis protein CcmE HAMAP-Rule MF_01959		PRO_0000201581
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	30 – 156	127	Periplasmic Potential
Sites
	Metal binding	128	1	Iron (heme axial ligand) By similarity
	Binding site	124	1	Heme (covalent; via pros nitrogen) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C1F6 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: D2AEAADD2D2CC2A5	FASTA	156	16,967
	10 20 30 40 50 60 MNPLRRKRLL IILAILAGVG IAVGLAMSAL RENINLFYTP TQIANGEAPL DTRIRAGGMV 70 80 90 100 110 120 EQGSLKRSGD SLDVTFVVTD FNKAVTITYR GILPDLFREG QGIVALGKLN ADAVVVADEV 130 140 150 LAKHDEKYMP PEVTKALKDS GQSRLARIRS LPRRAK

References

[1]

"A chromosomal locus required for copper resistance, competitive fitness, and cytochrome c biogenesis in Pseudomonas fluorescens." Yang C.-H., Azad H.R., Cooksey D.A. Proc. Natl. Acad. Sci. U.S.A. 93:7315-7320(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 09906.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U44827 Genomic DNA. Translation: AAC44225.1. Different initiation.
3D structure databases
ProteinModelPortal	P0C1F6.
SMR	P0C1F6. Positions 30-137.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_01959. CcmE.
InterPro	IPR004329. CcmE. IPR012340. NA-bd_OB-fold. [Graphical view]
Pfam	PF03100. CcmE. 1 hit. [Graphical view]
SUPFAM	SSF82093. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CCME_PSEFL

Accession

Primary (citable) accession number: P0C1F6
Secondary accession number(s): P52224

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	May 30, 2006
Last modified:	April 3, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program