P0C1E3 (PROB_CORML) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:

proB

Organism

Corynebacterium melassecola

Taxonomic identifier

41643 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Glutamate 5-kinase HAMAP-Rule MF_00456		PRO_0000236037
Regions
	Domain	277 – 351	75	PUA
	Nucleotide binding	175 – 176	2	ATP By similarity
	Nucleotide binding	215 – 221	7	ATP By similarity
Sites
	Binding site	14	1	ATP By similarity
	Binding site	56	1	Substrate By similarity
	Binding site	143	1	Substrate By similarity
	Binding site	155	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C1E3 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: E7CC949DBBDFC77F	FASTA	369	38,626
	10 20 30 40 50 60 MRERISNAKR VVVKIGSSSL TNDEDGHTVD PNRINTIVNA LQARMEAGSD LIVVSSGAVA 70 80 90 100 110 120 AGMAPLGLST RPTELAVKQA AAAVGQVHLM HQWGRSFARY GRPIGQVLLT AADAGKRDRA 130 140 150 160 170 180 RNAQRTIDKL RILGAVPIVN ENDTVATTGV NFGDNDRLAA IVAHLVSADA LVLLSDVDGL 190 200 210 220 230 240 FDKNPTDPTA KFISEVRDGN DLKGVIAGDG GKVGTGGMAS KVSAARLASR SGVPVLLTSA 250 260 270 280 290 300 ANIGPALEDA QVGTVFHPKD NRLSAWKFWA LYAADTAGKI RLDDGAVEAV TSGGKSLLAV 310 320 330 340 350 360 GITEIIGDFQ QGEIVEILGP AGQIIGRGEV SYDSDTLQSM VGMQTQDLPD GMQRPVVHAD YLSNYASRA

References

[1]

"Mutations in the Corynebacterium glutamicum proline biosynthetic pathway: a natural bypass of the proA step." Ankri S., Serebrijski I., Reyes O., Leblon G. J. Bacteriol. 178:4412-4419(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17965 / AS B-4821.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U31230 Genomic DNA. Translation: AAC44174.1.
PIR	T50666.
3D structure databases
ProteinModelPortal	P0C1E3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_CORML

Accession

Primary (citable) accession number: P0C1E3
Secondary accession number(s): P46546

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	May 16, 2006
Last modified:	April 3, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program