Reviewed, UniProtKB/Swiss-Prot P0C1C0 (PEL1_ERWCA)

Names and origin

Protein names

Recommended name:
    Pectate lyase 1
    EC=4.2.2.2
Alternative name(s):
    Pectate lyase I
      Short name=PEL I
    Pectate lyase A
      Short name=PLA

Gene names

Name:	pel1
Synonyms:	pelA

Organism

Erwinia carotovora

Taxonomic identifier

554 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted.
Sequence similarities	Belongs to the polysaccharide lyase 1 family. PLADES subfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.1
	Chain	23 – 374	352	Pectate lyase 1		PRO_0000024849
Sites
	Active site	239	1	Potential
	Metal binding	150	1	Calcium By similarity
	Metal binding	152	1	Calcium By similarity
	Metal binding	187	1	Calcium By similarity
	Metal binding	191	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	93 ↔ 176		By similarity
	Disulfide bond	350 ↔ 373		By similarity
Experimental info
	Sequence conflict	10	1	A → L in BAA00155. Ref.1
	Sequence conflict	14 – 22	9	LLAAQPTMA → AARGPTDNG in BAA00155. Ref.1
	Sequence conflict	112	1	I → V in BAA00155. Ref.1
	Sequence conflict	144 – 159	16	MPGGA…RIDNS → IRAAQKMAMPSVLITR AA sequence Ref.1
	Sequence conflict	331	1	K → N in BAA00155. Ref.1
	Sequence conflict	365 – 374	10	LAVLTAANCK → WRY in BAA00155. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0C1C0-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 7E60A2128D14E0D6	FASTA	374	40,153
	10 20 30 40 50 60 MKYLLPSAAA GLLLLAAQPT MAANTGGYAT TDGGDVSGAV KKTARSLQEI VDIIEAAKKD 70 80 90 100 110 120 SSGKAVKGGA YPLVITYNGN EDALIKAAEA NICGQWSKDP RGVEIKEFTK GITILGTNGS 130 140 150 160 170 180 SANFGIWMVN SSNVVVRNMR FGYMPGGAKD GDAIRIDNSP NVWIDHNEIF AKNFECAGTP 190 200 210 220 230 240 DNDTTFESAV DIKKGATNVT VSYNYIHGVK KVGLSGSSNT DTGRDLTYHH NIYSDVNSRL 250 260 270 280 290 300 PLQRGGKVHA YNNLYDGIKS SGFNVRQKGI ALIESNWFEN ALNPVTARND DSNFGTWELR 310 320 330 340 350 360 NNNITSPSDF AKYKITWGKP STPHINADDW KSTGKFPAVP YSYSPVSAQC VKDKLASYAG 370 VGKNLAVLTA ANCK

References

[1]	"DNA structure of pectate lyase I gene cloned from Erwinia carotovora." Ito K., Kobayashi R., Nikaido N., Izaki K. Agric. Biol. Chem. 52:479-487(1988) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-32. Strain: Er.
[2]	"Molecular cloning and sequencing of the extracellular pectate lyase II gene from Erwinia carotovora Er." Yoshida A., Matsuo Y., Kamio Y., Izaki K. Biosci. Biotechnol. Biochem. 56:1596-1600(1992) [PubMed: 1369060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION. Strain: Er.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00217 Genomic DNA. Translation: BAA00155.1. S51490 Genomic DNA. Translation: AAC60423.1.
PIR	JC1314.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Enzyme and pathway databases
BRENDA	4.2.2.2. 1498.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. SM00710. PbH1. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEL1_ERWCA

Accession

Primary (citable) accession number: P0C1C0
Secondary accession number(s): P11430 , P16529, Q06113, Q47468

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	May 16, 2006
Last modified:	November 24, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)