P0C1B6 (BEST_PARTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Bestoxin
Organism	Parabuthus transvaalicus (South African fattail scorpion)
Taxonomic identifier	170972 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Parabuthus

Protein attributes

Sequence length	58 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing By similarity. In mice, causes intense writhing.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the long (3 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily.
Mass spectrometry	Molecular mass is 6603.6 Da from positions 1 - 58. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Sodium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sodium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 58	58	Bestoxin		PRO_0000233629
Amino acid modifications
	Disulfide bond	18 ↔ 41		By similarity
	Disulfide bond	27 ↔ 46		By similarity
	Disulfide bond	31 ↔ 48		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C1B6 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: A5FD4ED9FC7794C2	FASTA	58	6,607
	10 20 30 40 50 ADVPGNYPLD KDGNTYTCLE LGENKDCQKV CKLHGVQYGY CYAFSCWCKE YLDDKDSV

References

[1]

"Three structurally related, highly potent, peptides from the venom of Parabuthus transvaalicus possess divergent biological activity." Inceoglu A.B., Lango J., Pessah I.N., Hammock B.D. Toxicon 45:727-733(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Venom.

Cross-references

3D structure databases
ProteinModelPortal	P0C1B6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.30.10. 1 hit.
InterPro	IPR003614. Scorpion_toxin-like. IPR002061. Scorpion_toxinL/defesin. [Graphical view]
Pfam	PF00537. Toxin_3. 1 hit. [Graphical view]
SMART	SM00505. Knot1. 1 hit. [Graphical view]
SUPFAM	SSF57095. SSF57095. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BEST_PARTR

Accession

Primary (citable) accession number: P0C1B6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	May 1, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program