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P0C1A8

- PMEA_DICCH

UniProt

P0C1A8 - PMEA_DICCH

Protein

Pectinesterase A

Gene

pemA

Organism
Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Involved in maceration and soft-rotting of plant tissue.By similarity

    Catalytic activityi

    Pectin + n H2O = n methanol + pectate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091SubstrateBy similarity
    Binding sitei153 – 1531SubstrateBy similarity
    Sitei177 – 1771Transition state stabilizerBy similarity
    Active sitei178 – 1781Proton donor
    Active sitei199 – 1991Nucleophile
    Binding sitei219 – 2191SubstrateBy similarity
    Binding sitei226 – 2261SubstrateBy similarity
    Binding sitei230 – 2301SubstrateBy similarity
    Binding sitei267 – 2671SubstrateBy similarity
    Binding sitei269 – 2691SubstrateBy similarity
    Binding sitei272 – 2721Substrate; via amide nitrogen and carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. aspartyl esterase activity Source: UniProtKB-KW
    2. pectinesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell wall modification Source: InterPro
    2. pectin catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aspartyl esterase, Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    SABIO-RKP0C1A8.
    UniPathwayiUPA00545; UER00823.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pectinesterase A (EC:3.1.1.11)
    Short name:
    PE A
    Alternative name(s):
    Pectin methylesterase A
    Gene namesi
    Name:pemA
    Synonyms:pem
    OrganismiDickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
    Taxonomic identifieri556 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeDickeya

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. cell wall Source: InterPro
    2. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Add
    BLAST
    Chaini25 – 366342Pectinesterase APRO_0000023498Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi192 ↔ 212

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    Secondary structure

    1
    366
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi29 – 324
    Beta strandi36 – 405
    Beta strandi42 – 443
    Helixi45 – 506
    Beta strandi54 – 574
    Beta strandi59 – 635
    Beta strandi65 – 684
    Beta strandi72 – 743
    Beta strandi79 – 846
    Turni86 – 883
    Beta strandi89 – 935
    Helixi108 – 1114
    Beta strandi114 – 1174
    Beta strandi119 – 1213
    Beta strandi123 – 1319
    Helixi135 – 1395
    Beta strandi156 – 1594
    Beta strandi165 – 17410
    Beta strandi180 – 1823
    Beta strandi184 – 1918
    Beta strandi193 – 21119
    Beta strandi213 – 2164
    Beta strandi229 – 2346
    Beta strandi244 – 2485
    Beta strandi250 – 2556
    Beta strandi263 – 2664
    Beta strandi272 – 2754
    Beta strandi278 – 2814
    Beta strandi288 – 2936
    Beta strandi300 – 3023
    Beta strandi306 – 3094
    Beta strandi315 – 3184
    Helixi320 – 3223
    Beta strandi323 – 3297
    Beta strandi337 – 3415
    Helixi346 – 3494
    Helixi350 – 3523
    Helixi354 – 3585

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1QJVX-ray2.37A/B25-366[»]
    ProteinModelPortaliP0C1A8.
    SMRiP0C1A8. Positions 25-366.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0C1A8.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pectinesterase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.160.20.10. 1 hit.
    InterProiIPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    IPR018040. Pectinesterase_AS.
    IPR000070. Pectinesterase_cat.
    [Graphical view]
    PfamiPF01095. Pectinesterase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51126. SSF51126. 1 hit.
    PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0C1A8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKTISGTLA LSLIIAASVH QAQAATTYNA VVSKSSSDGK TFKTIADAIA    50
    SAPAGSTPFV ILIKNGVYNE RLTITRNNLL LKGESRNGAV IAAATAAGTL 100
    KSDGSKWGTA GSSTITISAK DFSAQSLTIR NDFDFPANQA KSDSDSSKIK 150
    DTQAVALYVT KSGDRAYFKD VSLVGYQDTL YVSGGRSFFS DCRISGTVDF 200
    IFGDGTALFN NCDLVSRYRA DVKSGNVSGY LTAPSTNINQ KYGLVITNSR 250
    VIRESDSVPA KSYGLGRPWH PTTTFSDGRY ADPNAIGQTV FLNTSMDNHI 300
    YGWDKMSGKD KNGNTIWFNP EDSRFFEYKS YGAGAAVSKD RRQLTDAQAA 350
    EYTQSKVLGD WTPTLP 366
    Length:366
    Mass (Da):39,319
    Last modified:April 18, 2006 - v1
    Checksum:iDA7DD9A45290660B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00549 Genomic DNA. Translation: CAA68628.1.
    PIRiS03770.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00549 Genomic DNA. Translation: CAA68628.1 .
    PIRi S03770.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1QJV X-ray 2.37 A/B 25-366 [» ]
    ProteinModelPortali P0C1A8.
    SMRi P0C1A8. Positions 25-366.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00545 ; UER00823 .
    SABIO-RK P0C1A8.

    Miscellaneous databases

    EvolutionaryTracei P0C1A8.

    Family and domain databases

    Gene3Di 2.160.20.10. 1 hit.
    InterProi IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    IPR018040. Pectinesterase_AS.
    IPR000070. Pectinesterase_cat.
    [Graphical view ]
    Pfami PF01095. Pectinesterase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51126. SSF51126. 1 hit.
    PROSITEi PS00503. PECTINESTERASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence of the pectin methyl esterase gene of Erwinia chrysanthemi B374."
      Plastow G.S.
      Mol. Microbiol. 2:247-254(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: B374.
    2. "Three-dimensional structure of Erwinia chrysanthemi pectin methylesterase reveals a novel esterase active site."
      Jenkins J., Mayans O., Smith D., Worboys K., Pickersgill R.W.
      J. Mol. Biol. 305:951-960(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.37 ANGSTROMS) OF 25-366.
      Strain: B374.

    Entry informationi

    Entry nameiPMEA_DICCH
    AccessioniPrimary (citable) accession number: P0C1A8
    Secondary accession number(s): P07863
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2006
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3