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P0C1A6

- PLYL_DICCH

UniProt

P0C1A6 - PLYL_DICCH

Protein

Pectate lyase L

Gene

pelL

Organism
Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Presents an endo-cleaving activity on polygalacturonate or partially methylated pectin.By similarity

    Catalytic activityi

    Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

    Cofactori

    Calcium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi209 – 2091Calcium 1By similarity
    Metal bindingi233 – 2331Calcium 1By similarity
    Metal bindingi234 – 2341Calcium 1By similarity
    Metal bindingi237 – 2371Calcium 1By similarity
    Active sitei273 – 2731Proton acceptorBy similarity
    Metal bindingi402 – 4021Calcium 2By similarity
    Metal bindingi413 – 4131Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi416 – 4161Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi418 – 4181Calcium 2By similarity
    Metal bindingi423 – 4231Calcium 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. pectate lyase activity Source: UniProtKB-EC

    GO - Biological processi

    1. pectin catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00545; UER00824.

    Protein family/group databases

    CAZyiPL9. Polysaccharide Lyase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pectate lyase L (EC:4.2.2.2)
    Alternative name(s):
    Pectate transeliminase
    Gene namesi
    Name:pelL
    OrganismiDickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
    Taxonomic identifieri556 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeDickeya

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 425400Pectate lyase LPRO_0000024936Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi28 ↔ 114By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiP0C1A6.

    Structurei

    3D structure databases

    ProteinModelPortaliP0C1A6.
    SMRiP0C1A6. Positions 26-425.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the polysaccharide lyase 9 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.160.20.10. 1 hit.
    InterProiIPR006626. PbH1.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view]
    SMARTiSM00710. PbH1. 5 hits.
    [Graphical view]
    SUPFAMiSSF51126. SSF51126. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0C1A6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKYLNCFIST GLAAFFLVNS TSVLAADCSS DLTSGISTKR IYYVAPNGSS    50
    SNNGNSFNSP MSFTAAMAAA NPGELILLKP GTYTIPYTQG KGNTITFNKS 100
    GKEGSPIYVA AANCGRAVFD FSFPDSQWVQ ASYGFYVTGD YWYFKGIEVT 150
    RAGYQGAYVT GSHNTFENTA FHHNRNTGLE INNGGSYNTV INSDAYRNYD 200
    PKKNGSMADG FGPKQKQGQG NRFGGCRAWE NSDDGFDLFD SPQKVVIENS 250
    WAFRNGINYW SDSSFAGNGN GFKLGGNQAV GNHRITRSVA FGNVSKGFDQ 300
    NNNAGGVTVI NNTSYKNGIN YGFGSNVKSG QKHYFRNNVS LSGSATVNNA 350
    DAKSNSWDTG PVASASDFVS LDTSLATISR DNDGTLPETA LFRLSTNSKL 400
    INAGTKESNI SYSGSAPDLG AFERN 425
    Length:425
    Mass (Da):45,537
    Last modified:April 18, 2006 - v1
    Checksum:iBE2557332CFF91F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42248 Genomic DNA. Translation: AAA99476.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42248 Genomic DNA. Translation: AAA99476.1 .

    3D structure databases

    ProteinModelPortali P0C1A6.
    SMRi P0C1A6. Positions 26-425.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi PL9. Polysaccharide Lyase Family 9.

    Proteomic databases

    PRIDEi P0C1A6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00545 ; UER00824 .

    Family and domain databases

    Gene3Di 2.160.20.10. 1 hit.
    InterProi IPR006626. PbH1.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view ]
    SMARTi SM00710. PbH1. 5 hits.
    [Graphical view ]
    SUPFAMi SSF51126. SSF51126. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes."
      Alfano J.R., Ham J.H., Collmer A.
      J. Bacteriol. 177:4553-4556(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: EC16.

    Entry informationi

    Entry nameiPLYL_DICCH
    AccessioniPrimary (citable) accession number: P0C1A6
    Secondary accession number(s): Q47473, Q59421
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2006
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3