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Protein

Pectate lyase L

Gene

pelL

Organism
Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presents an endo-cleaving activity on polygalacturonate or partially methylated pectin.By similarity

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactori

Ca2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi209 – 2091Calcium 1By similarity
Metal bindingi233 – 2331Calcium 1By similarity
Metal bindingi234 – 2341Calcium 1By similarity
Metal bindingi237 – 2371Calcium 1By similarity
Active sitei273 – 2731Proton acceptorBy similarity
Metal bindingi402 – 4021Calcium 2By similarity
Metal bindingi413 – 4131Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi416 – 4161Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi418 – 4181Calcium 2By similarity
Metal bindingi423 – 4231Calcium 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. pectate lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. pectin catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Protein family/group databases

CAZyiPL9. Polysaccharide Lyase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectate lyase L (EC:4.2.2.2)
Alternative name(s):
Pectate transeliminase
Gene namesi
Name:pelL
OrganismiDickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
Taxonomic identifieri556 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeDickeya

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 425400Pectate lyase LPRO_0000024936Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 114By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP0C1A6.

Structurei

3D structure databases

ProteinModelPortaliP0C1A6.
SMRiP0C1A6. Positions 26-425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polysaccharide lyase 9 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C1A6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYLNCFIST GLAAFFLVNS TSVLAADCSS DLTSGISTKR IYYVAPNGSS
60 70 80 90 100
SNNGNSFNSP MSFTAAMAAA NPGELILLKP GTYTIPYTQG KGNTITFNKS
110 120 130 140 150
GKEGSPIYVA AANCGRAVFD FSFPDSQWVQ ASYGFYVTGD YWYFKGIEVT
160 170 180 190 200
RAGYQGAYVT GSHNTFENTA FHHNRNTGLE INNGGSYNTV INSDAYRNYD
210 220 230 240 250
PKKNGSMADG FGPKQKQGQG NRFGGCRAWE NSDDGFDLFD SPQKVVIENS
260 270 280 290 300
WAFRNGINYW SDSSFAGNGN GFKLGGNQAV GNHRITRSVA FGNVSKGFDQ
310 320 330 340 350
NNNAGGVTVI NNTSYKNGIN YGFGSNVKSG QKHYFRNNVS LSGSATVNNA
360 370 380 390 400
DAKSNSWDTG PVASASDFVS LDTSLATISR DNDGTLPETA LFRLSTNSKL
410 420
INAGTKESNI SYSGSAPDLG AFERN
Length:425
Mass (Da):45,537
Last modified:April 18, 2006 - v1
Checksum:iBE2557332CFF91F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42248 Genomic DNA. Translation: AAA99476.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42248 Genomic DNA. Translation: AAA99476.1.

3D structure databases

ProteinModelPortaliP0C1A6.
SMRiP0C1A6. Positions 26-425.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiPL9. Polysaccharide Lyase Family 9.

Proteomic databases

PRIDEiP0C1A6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes."
    Alfano J.R., Ham J.H., Collmer A.
    J. Bacteriol. 177:4553-4556(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: EC16.

Entry informationi

Entry nameiPLYL_DICCH
AccessioniPrimary (citable) accession number: P0C1A6
Secondary accession number(s): Q47473, Q59421
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: April 18, 2006
Last modified: January 7, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.