P0C1A6 (PLYL_ERWCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pectate lyase L
EC=4.2.2.2

Alternative name(s):
Pectate transeliminase

Gene names

Name:

pelL

Organism

Erwinia chrysanthemi

Taxonomic identifier

556 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presents an endo-cleaving activity on polygalacturonate or partially methylated pectin By similarity.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Calcium By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the polysaccharide lyase 9 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Disulfide bond
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 425	400	Pectate lyase L		PRO_0000024936
Sites
	Active site	273	1	Proton acceptor By similarity
	Metal binding	209	1	Calcium 1 By similarity
	Metal binding	233	1	Calcium 1 By similarity
	Metal binding	234	1	Calcium 1 By similarity
	Metal binding	237	1	Calcium 1 By similarity
	Metal binding	402	1	Calcium 2 By similarity
	Metal binding	413	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	416	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	418	1	Calcium 2 By similarity
	Metal binding	423	1	Calcium 2 By similarity
Amino acid modifications
	Disulfide bond	28 ↔ 114		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C1A6 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: BE2557332CFF91F7	FASTA	425	45,537
	10 20 30 40 50 60 MKYLNCFIST GLAAFFLVNS TSVLAADCSS DLTSGISTKR IYYVAPNGSS SNNGNSFNSP 70 80 90 100 110 120 MSFTAAMAAA NPGELILLKP GTYTIPYTQG KGNTITFNKS GKEGSPIYVA AANCGRAVFD 130 140 150 160 170 180 FSFPDSQWVQ ASYGFYVTGD YWYFKGIEVT RAGYQGAYVT GSHNTFENTA FHHNRNTGLE 190 200 210 220 230 240 INNGGSYNTV INSDAYRNYD PKKNGSMADG FGPKQKQGQG NRFGGCRAWE NSDDGFDLFD 250 260 270 280 290 300 SPQKVVIENS WAFRNGINYW SDSSFAGNGN GFKLGGNQAV GNHRITRSVA FGNVSKGFDQ 310 320 330 340 350 360 NNNAGGVTVI NNTSYKNGIN YGFGSNVKSG QKHYFRNNVS LSGSATVNNA DAKSNSWDTG 370 380 390 400 410 420 PVASASDFVS LDTSLATISR DNDGTLPETA LFRLSTNSKL INAGTKESNI SYSGSAPDLG AFERN

References

[1]

"Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes." Alfano J.R., Ham J.H., Collmer A. J. Bacteriol. 177:4553-4556(1995) [PubMed: 7635842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EC16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42248 Genomic DNA. Translation: AAA99476.1.
3D structure databases
ProteinModelPortal	P0C1A6.
SMR	P0C1A6. Positions 26-425.
ModBase	Search...
Protein family/group databases
CAZy	PL9. Polysaccharide Lyase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
SMART	SM00710. PbH1. 5 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLYL_ERWCH

Accession

Primary (citable) accession number: P0C1A6
Secondary accession number(s): Q47473, Q59421

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program