P0C1A5 (PLYE_DICD3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pectate lyase E
EC=4.2.2.2

Gene names

Name:	pelE
Ordered Locus Names:	Dda3937_03371

Organism

Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) [Complete proteome] [HAMAP]

Taxonomic identifier

198628 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue. Pectate lyases have been implicated as pathogenicity factors which induce maceration or rotting of plant tissue. PelE is sufficient to induce these effects under laboratory conditions.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted.
Sequence similarities	Belongs to the polysaccharide lyase 1 family. PLBC subfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 41	41	By similarity
	Chain	42 – 404	363	Pectate lyase E		PRO_0000233031
Sites
	Active site	278	1	Potential
	Metal binding	182	1	Calcium By similarity
	Metal binding	225	1	Calcium By similarity
Experimental info
	Sequence conflict	114	1	S → D in AAA24854. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0C1A5 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: 59DB45FD21FAA8E4	FASTA	404	43,066
	10 20 30 40 50 60 MNNSRMSSVS TQKTTGRSAL GTKSALAAII ATTMMVSVAS AASLQTTKAT EAASTGWATQ 70 80 90 100 110 120 SGGTTGGAKA SSSKIYAVKS ISEFKAALNG TDSSPKIIQV TGAIDISGGK AYTSFDDQKA 130 140 150 160 170 180 RSQISIPSNT TIIGIGNKGK FTNGSLVVKG VSNVILRNLY IETPVDVAPH YEEGDGWNAE 190 200 210 220 230 240 WDAVVIDSTD HVWVDHVTIS DGSLTDDKYT TKNGEKYVQH DGSLDIKRGS DYVTVSNSRF 250 260 270 280 290 300 ELHDKTILIG HSDNNGSQDA GKLRVTFHNN LFDRVGERTP RVRFGSVHAY NNVYVGDVNH 310 320 330 340 350 360 KAYRYQYSFG IGTSGSLLSE SNAFTIDNMK KISGRDKECS VVKAFNGKIF SDKGSIINGA 370 380 390 400 SYNLNGCGFG FSAYSAKIPY KYSAQTITTS LANSISSNAG YGKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequences of the Erwinia chrysanthemi ogl and pelE genes negatively regulated by the kdgR gene product." Reverchon S., Huang Y., Bourson C., Robert-Baudouy J. Gene 85:125-134(1989) [PubMed: 2695393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 3937.
[2]	"Complete genome sequence of Dickeya dadantii 3937." International Erwinia Consortium Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 3937.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33584 Genomic DNA. Translation: AAA24854.1. CP002038 Genomic DNA. Translation: ADM99551.1.
PIR	WZWCPE. JQ0190.
RefSeq	YP_003884108.1. NC_014500.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9734802.
GenomeReviews	Gene locus Dda3937_03371 in contig CP002038_GR.
KEGG	ddd:Dda3937_03371.
PATRIC	42318993. VBIDicDad25310_3309.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15660.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01728.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLYE_DICD3

Accession

Primary (citable) accession number: P0C1A5
Secondary accession number(s): E0SAZ2, P18210

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	November 30, 2010
Last modified:	January 25, 2012
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program