P0C1A4 (PLYE2_ERWCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pectate lyase E
EC=4.2.2.2

Gene names

Name:

pelE

Organism

Erwinia chrysanthemi

Taxonomic identifier

556 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue. Pectate lyases have been implicated as pathogenicity factors which induce maceration or rotting of plant tissue. PelE is sufficient to induce these effects under laboratory conditions.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted.
Sequence similarities	Belongs to the polysaccharide lyase 1 family. PLBC subfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 41	41	By similarity
	Chain	42 – 404	363	Pectate lyase E		PRO_0000024858
Sites
	Active site	278	1	Potential
	Metal binding	182	1	Calcium By similarity
	Metal binding	225	1	Calcium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C1A4 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: B17C8234445D6AEC	FASTA	404	43,043
	10 20 30 40 50 60 MNNSRMSSVS TQKTTGRSAL GTKSALAAII ATTMMVSVAS AASLQTTKAT EAASTGWATQ 70 80 90 100 110 120 SGGTTGGAKA SSSKIYAVKS ISEFKAALNG TDSSPKIIQV TGAIDISGGK AYTSFDDQKA 130 140 150 160 170 180 RSQISIPSNT TIIGIGNKGK FTNGSLVVKG VSNVILRNLY IETPVDVAPH YEEGDGWNAE 190 200 210 220 230 240 WDAVVIDSTD HVWVDHVTIS DGSFTDDKYT TKNGEKYVQH DGSLDIKRGS DYVTVSNSRF 250 260 270 280 290 300 ELHDKTILIG HSDNNGSQDA GKLRVTFHNN LFDRVGERTP RVRFGSVHAY NNVYVGDVNH 310 320 330 340 350 360 KAYRYQYSFG IGTSGSLLSE SNAFTIDNMK KISGRDKECS VVKAFNGKIF SDKGSIINGA 370 380 390 400 SYNLNGCGFG FSAYSAKIPY KYSAQTITTS LAGSISSNAG YGKL

References

[1]	"Relationship between the pel genes of the pelADE cluster in Erwinia chrysanthemi strain B374." van Gijsegem F. Mol. Microbiol. 3:1415-1424(1989) [PubMed: 2615652] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B374.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17284 Genomic DNA. Translation: CAA35175.1.
PIR	WZWCPE. JQ0190.
3D structure databases
ProteinModelPortal	P0C1A4.
SMR	P0C1A4. Positions 42-404.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLYE2_ERWCH

Accession

Primary (citable) accession number: P0C1A4
Secondary accession number(s): P18210

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	April 18, 2006
Last modified:	May 31, 2011
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program