P0C1A3 (PLYA_DICD3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pectate lyase A
EC=4.2.2.2

Gene names

Name:	pelA
Ordered Locus Names:	Dda3937_03370

Organism

Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) [Complete proteome] [HAMAP]

Taxonomic identifier

198628 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted.
Sequence similarities	Belongs to the polysaccharide lyase 1 family. PLADES subfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 392	361	Pectate lyase A		PRO_0000233030
Sites
	Active site	272	1	Potential
	Metal binding	173	1	Calcium By similarity
	Metal binding	175	1	Calcium By similarity
	Metal binding	215	1	Calcium By similarity
	Metal binding	219	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	329 ↔ 357		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C1A3 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 7EC15FB476B9D333	FASTA	392	41,555
	10 20 30 40 50 60 MNKVSGRSFT RTSTCLLATL IAGVMTSGVS AAELVNSKAL ESAPAAGWAS QNGSTTGGAA 70 80 90 100 110 120 ATSDNIYVVT NISEFTSALS AGAVAKIIQI TGTVDISGGT PYKDFADQKA RSQINIPANT 130 140 150 160 170 180 TVIGIGTDAK FINGSLIIDG TDGTNNVIIR NVYIQTPIDV EPHYEKGDGW NAEWDGMNIT 190 200 210 220 230 240 NGAHHVWVDH VTISDGSFTD DMYTTKDGET YVQHDGALDI KRGSDYVTIS NSLFDQHDKT 250 260 270 280 290 300 MLIGHSDTNS AQDKGKLHVT LFNNVFNRVT ERAPRVRYGS IHSFNNVFNG DVKDPVYRYL 310 320 330 340 350 360 YSFGIGTSGS VLSEGNSFTI ANLSASKACK VVKKFNGSIF SDNGSVLNGS AADLSGCGFS 370 380 390 AYTSAIPYVY AVQPMTTELA QSITDHAGSG KL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification of the acidic pectate lyase and nucleotide sequence of the corresponding gene (pelA) of Erwinia chrysanthemi strain 3937." Favey S., Bourson C., Bertheau Y., Kotoujansky A., Boccara M. J. Gen. Microbiol. 138:499-508(1992) [PubMed: 1593262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 3937.
[2]	"Complete genome sequence of Dickeya dadantii 3937." International Erwinia Consortium Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 3937.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M77808 Genomic DNA. Translation: AAA24846.1. CP002038 Genomic DNA. Translation: ADM99550.1.
PIR	A44852.
RefSeq	YP_003884107.1. NC_014500.1.
3D structure databases
ProteinModelPortal	P0C1A3.
SMR	P0C1A3. Positions 32-392.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9734801.
GenomeReviews	Gene locus Dda3937_03370 in contig CP002038_GR.
KEGG	ddd:Dda3937_03370.
PATRIC	42318989. VBIDicDad25310_3307.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15663.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLYA_DICD3

Accession

Primary (citable) accession number: P0C1A3
Secondary accession number(s): E0SAZ1, P29155

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program