ID   ASSY_DICCH              Reviewed;         242 AA.
AC   P0C1A0; P42181; Q9KHB9;
DT   18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   24-JAN-2024, entry version 50.
DE   RecName: Full=Argininosuccinate synthase;
DE            EC=6.3.4.5;
DE   AltName: Full=Citrulline--aspartate ligase;
DE   Flags: Fragment;
GN   Name=argG;
OS   Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Dickeya.
OX   NCBI_TaxID=556;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RA3B;
RA   Huang H.-C., Chu M.-K., Hsu S.-T., Tzeng K.-C., Lin R.-H.;
RT   "Molecular cloning of genes involved in blue pigment synthesis from Erwinia
RT   chrysanthemi RA3B.";
RL   Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-
CC         arginino)succinate + AMP + diphosphate + H(+); Xref=Rhea:RHEA:10932,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29991, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57472, ChEBI:CHEBI:57743,
CC         ChEBI:CHEBI:456215; EC=6.3.4.5;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine
CC       from L-ornithine and carbamoyl phosphate: step 2/3.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the argininosuccinate synthase family. Type 2
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AF265211; AAF74776.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0C1A0; -.
DR   SMR; P0C1A0; -.
DR   UniPathway; UPA00068; UER00113.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004055; F:argininosuccinate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:InterPro.
DR   GO; GO:0006526; P:arginine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.287.400; -; 1.
DR   Gene3D; 3.90.1260.10; Argininosuccinate synthetase, chain A, domain 2; 1.
DR   InterPro; IPR048268; Arginosuc_syn_C.
DR   InterPro; IPR001518; Arginosuc_synth.
DR   InterPro; IPR024074; AS_cat/multimer_dom_body.
DR   InterPro; IPR024073; AS_multimer_C_tail.
DR   PANTHER; PTHR11587; ARGININOSUCCINATE SYNTHASE; 1.
DR   PANTHER; PTHR11587:SF2; ARGININOSUCCINATE SYNTHASE; 1.
DR   Pfam; PF20979; Arginosuc_syn_C; 1.
DR   SUPFAM; SSF69864; Argininosuccinate synthetase, C-terminal domain; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding; Cytoplasm;
KW   Ligase; Nucleotide-binding.
FT   CHAIN           <1..242
FT                   /note="Argininosuccinate synthase"
FT                   /id="PRO_0000148699"
FT   NON_TER         1
SQ   SEQUENCE   242 AA;  27349 MW;  BF3C91A7FD7D49D5 CRC64;
     EFLNSSVKIV NPIMGVKFWD ENVRIPAEEV TVRFERGHPV ALNGQTFSDD VELLLEANRI
     GGRHGLGMSD QIENRIIEAK SRGIYEAPGM ALLHIAYERL VTGIHNEDTI EQYHAHGRQL
     GRLLYQGRWF DPQALMLRDA LQRWVASEIT GEVTLELRRG NDYSILNTVS DNLTYKPERL
     TMEKGESVFS PDDRIGQLTM RNLDITDTRE KLFNYVESGL ISSGNAGLPQ VANPLLQDKS
     AK
//