P0C195 (CM3A_CONKI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Mu-conotoxin KIIIA
Organism	Conus kinoshitai (Kinoshita's cone)
Taxonomic identifier	376876 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Hypsogastropoda › Neogastropoda › Conoidea › Conidae › Conus

Protein attributes

Sequence length	18 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mu-conotoxins block voltage-gated sodium channels (VGSC). This toxin blocks tetrodotoxin(TTX)-resistant VGSCs, without blocking TTX-sensitive VGSCs. Also reversibly inhibits sodium channels Nav1.4/SCN4A. Ref.1 Ref.3 Ref.4
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed by the venom duct.
Domain	The cysteine framework is III (CC-C-C-CC). Classified in the M-5 branch, since 5 residues stand between the fourth and the fifth cysteine residues.
Sequence similarities	Belongs to the conotoxin M superfamily.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Sodium channel inhibitor Toxin
PTM	Amidation Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sodium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Peptide

1 – 16

16

Mu-conotoxin KIIIA

PRO_0000232662

Amino acid modifications

Modified residue

16

1

Cysteine amide

Disulfide bond

1 ↔ 9

By similarity

Disulfide bond

2 ↔ 15

By similarity

Disulfide bond

4 ↔ 16

By similarity

Secondary structure

1

.

.

.

18

Helix Strand Turn

Details...

Helix

7 – 12

6

Sequences

	Sequence		Length	Mass (Da)
	P0C195 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 6FA593BD458C3C39	FASTA	18	2,104
	10 CCNCSSKWCR DHSRCCGR

References

[1]	"Novel conotoxins from Conus striatus and Conus kinoshitai selectively block TTX-resistant sodium channels." Bulaj G., West P.J., Garrett J.E., Watkins M., Zhang M.-M., Norton R.S., Smith B.J., Yoshikami D., Olivera B.M. Biochemistry 44:7259-7265(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF 1-16, FUNCTION. Tissue: Venom duct.
[2]	Erratum Bulaj G., West P.J., Garrett J.E., Watkins M., Zhang M.-M., Norton R.S., Smith B.J., Yoshikami D., Olivera B.M. Biochemistry 45:3116-3116(2006)
[3]	"Structure/function characterization of mu-conotoxin KIIIA, an analgesic, nearly irreversible blocker of mammalian neuronal sodium channels." Zhang M.-M., Green B.R., Catlin P., Fiedler B., Azam L., Chadwick A., Terlau H., McArthur J.R., French R.J., Gulyas J., Rivier J.E., Smith B.J., Norton R.S., Olivera B.M., Yoshikami D., Bulaj G. J. Biol. Chem. 282:30699-30706(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Pruning nature: biodiversity-derived discovery of novel sodium channel blocking conotoxins from Conus bullatus." Holford M., Zhang M.-M., Gowd K.H., Azam L., Green B.R., Watkins M., Ownby J.-P., Yoshikami D., Bulaj G., Olivera B.M. Toxicon 53:90-98(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LXG	NMR	-	A	1-16	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

ConoServer

1694. KIIIA precursor.

Family and domain databases

ProtoNet

Search...

Entry information

Entry name

CM3A_CONKI

Accession

Primary (citable) accession number: P0C195

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	April 18, 2006
Last modified:	May 1, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program