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Protein

Leucine-rich repeat-containing protein 4B

Gene

Lrrc4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Synaptic adhesion protein. Regulates the formation of excitatory synapses. The trans-synaptic adhesion between LRRC4B and PTPRF regulates the formation of excitatory synapses in a bidirectional manner (By similarity).By similarity

GO - Molecular functioni

  • protein kinase inhibitor activity Source: GO_Central
  • receptor binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat-containing protein 4B
Alternative name(s):
Netrin-G3 ligand
Short name:
NGL-3
Gene namesi
Name:Lrrc4b
Synonyms:Lrig4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:3027390. Lrrc4b.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei575 – 595HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Sequence analysisAdd BLAST38
ChainiPRO_000023238739 – 709Leucine-rich repeat-containing protein 4BAdd BLAST671

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi226N-linked (GlcNAc...)Sequence analysis1
Glycosylationi285N-linked (GlcNAc...)Sequence analysis1
Glycosylationi335N-linked (GlcNAc...)Sequence analysis1
Glycosylationi376N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi387 ↔ 438PROSITE-ProRule annotation
Glycosylationi402N-linked (GlcNAc...)Sequence analysis1
Glycosylationi424N-linked (GlcNAc...)Sequence analysis1
Glycosylationi427N-linked (GlcNAc...)Sequence analysis1
Glycosylationi446N-linked (GlcNAc...)Sequence analysis1
Glycosylationi454N-linked (GlcNAc...)Sequence analysis1
Modified residuei689PhosphoserineCombined sources1

Post-translational modificationi

N-glycosylated. O-glycosylated; contains sialic acid.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP0C192.
PaxDbiP0C192.
PeptideAtlasiP0C192.
PRIDEiP0C192.

PTM databases

iPTMnetiP0C192.
PhosphoSitePlusiP0C192.

Expressioni

Gene expression databases

BgeeiENSMUSG00000047085.
CleanExiMM_LRRC4B.
ExpressionAtlasiP0C192. baseline and differential.
GenevisibleiP0C192. MM.

Interactioni

Subunit structurei

Interacts with PTPRF (By similarity). Interacts with DLG4.By similarity1 Publication

GO - Molecular functioni

  • receptor binding Source: BHF-UCL

Protein-protein interaction databases

IntActiP0C192. 1 interactor.
STRINGi10090.ENSMUSP00000053123.

Structurei

Secondary structure

1709
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi64 – 66Combined sources3
Turni67 – 69Combined sources3
Beta strandi71 – 73Combined sources3
Beta strandi91 – 94Combined sources4
Turni105 – 110Combined sources6
Beta strandi116 – 118Combined sources3
Turni129 – 132Combined sources4
Beta strandi140 – 142Combined sources3
Turni153 – 155Combined sources3
Beta strandi164 – 166Combined sources3
Turni177 – 182Combined sources6
Beta strandi187 – 190Combined sources4
Turni202 – 207Combined sources6
Beta strandi213 – 215Combined sources3
Beta strandi235 – 237Combined sources3
Helixi248 – 251Combined sources4
Beta strandi259 – 261Combined sources3
Turni272 – 277Combined sources6
Beta strandi283 – 285Combined sources3
Beta strandi307 – 309Combined sources3
Helixi319 – 321Combined sources3
Helixi322 – 331Combined sources10
Beta strandi341 – 345Combined sources5
Helixi346 – 348Combined sources3
Helixi353 – 355Combined sources3
Helixi358 – 360Combined sources3
Beta strandi367 – 370Combined sources4
Beta strandi379 – 381Combined sources3
Beta strandi383 – 385Combined sources3
Beta strandi388 – 399Combined sources12
Beta strandi423 – 427Combined sources5
Helixi430 – 432Combined sources3
Beta strandi434 – 441Combined sources8
Beta strandi446 – 453Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZYNX-ray3.20A/B57-365[»]
3ZYOX-ray3.10A57-455[»]
ProteinModelPortaliP0C192.
SMRiP0C192.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C192.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 88LRRNTAdd BLAST39
Repeati89 – 110LRR 1Add BLAST22
Repeati113 – 134LRR 2Add BLAST22
Repeati137 – 158LRR 3Add BLAST22
Repeati161 – 182LRR 4Add BLAST22
Repeati185 – 207LRR 5Add BLAST23
Repeati210 – 231LRR 6Add BLAST22
Repeati232 – 253LRR 7Add BLAST22
Repeati256 – 277LRR 8Add BLAST22
Repeati280 – 301LRR 9Add BLAST22
Domaini313 – 365LRRCTAdd BLAST53
Domaini366 – 454Ig-like C2-typeAdd BLAST89

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi39 – 42Poly-Gly4
Compositional biasi464 – 482Gly-richAdd BLAST19
Compositional biasi631 – 637Poly-Ala7

Domaini

The extreme C-terminus binds to the first 2 PDZ domains of DLG4.

Sequence similaritiesi

Contains 9 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000118831.
HOGENOMiHOG000252924.
HOVERGENiHBG052359.
InParanoidiP0C192.
KOiK16360.
OMAiTEGMGPG.
OrthoDBiEOG091G037N.
PhylomeDBiP0C192.
TreeFamiTF324303.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR026883. LRRC4B.
IPR000372. LRRNT.
[Graphical view]
PANTHERiPTHR24369:SF66. PTHR24369:SF66. 4 hits.
PfamiPF07679. I-set. 1 hit.
PF13855. LRR_8. 3 hits.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 8 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51450. LRR. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C192-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQAHIRGSP CPLLPPGRMS WPHGALLLLW LFSPPLRAGG GGVAVTSAAG
60 70 80 90 100
GGSPPATSCP AACSCSNQAS RVICTRRELA EVPASIPVNT RYLNLQENSI
110 120 130 140 150
QVIRTDTFKH LRHLEILQLS KNLVRKIEVG AFNGLPSLNT LELFDNRLTT
160 170 180 190 200
VPTQAFEYLS KLRELWLRNN PIESIPSYAF NRVPSLRRLD LGELKRLEYI
210 220 230 240 250
SEAAFEGLVN LRYLNLGMCN LKDIPNLTAL VRLEELELSG NRLDLIRPGS
260 270 280 290 300
FQGLTSLRKL WLMHAQVATI ERNAFDDLKS LEELNLSHNN LMSLPHDLFT
310 320 330 340 350
PLHRLERVHL NHNPWHCNCD VLWLSWWLKE TVPSNTTCCA RCHAPAGLKG
360 370 380 390 400
RYIGELDQSH FTCYAPVIVE PPTDLNVTEG MAAELKCRTG TSMTSVNWLT
410 420 430 440 450
PNGTLMTHGS YRVRISVLHD GTLNFTNVTV QDTGQYTCMV TNSAGNTTAS
460 470 480 490 500
ATLNVSAVDP VAAGGPGGGG PGGGGGAGGA GGYTYFTTVT VETLETQPGE
510 520 530 540 550
EAQQPRGTEK EPPGPTTDGA WGGGRPDAAA PASASTTAPA PRSSRPTEKA
560 570 580 590 600
FTVPITDVTE NALKDLDDVM KTTKIIIGCF VAITFMAAVM LVAFYKLRKQ
610 620 630 640 650
HQLHKHHGPT RTVEIINVED ELPAASAVSV AAAAAVAGGA GVGGDSHLAL
660 670 680 690 700
PALERDHLNH HHYVAAAFKA HYGGNPGGGC GAKGPGLNSI HEPLLFKSGS

KENVQETQI
Length:709
Mass (Da):76,156
Last modified:April 4, 2006 - v1
Checksum:i0C39148F9A8E3586
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti574K → R in BAE24378 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC060263 mRNA. Translation: AAH60263.1.
AK140418 mRNA. Translation: BAE24378.1.
CCDSiCCDS21206.1.
RefSeqiNP_937893.1. NM_198250.1.
UniGeneiMm.44173.

Genome annotation databases

EnsembliENSMUST00000058667; ENSMUSP00000053123; ENSMUSG00000047085.
GeneIDi272381.
KEGGimmu:272381.
UCSCiuc009gpj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC060263 mRNA. Translation: AAH60263.1.
AK140418 mRNA. Translation: BAE24378.1.
CCDSiCCDS21206.1.
RefSeqiNP_937893.1. NM_198250.1.
UniGeneiMm.44173.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZYNX-ray3.20A/B57-365[»]
3ZYOX-ray3.10A57-455[»]
ProteinModelPortaliP0C192.
SMRiP0C192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0C192. 1 interactor.
STRINGi10090.ENSMUSP00000053123.

PTM databases

iPTMnetiP0C192.
PhosphoSitePlusiP0C192.

Proteomic databases

MaxQBiP0C192.
PaxDbiP0C192.
PeptideAtlasiP0C192.
PRIDEiP0C192.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058667; ENSMUSP00000053123; ENSMUSG00000047085.
GeneIDi272381.
KEGGimmu:272381.
UCSCiuc009gpj.1. mouse.

Organism-specific databases

CTDi94030.
MGIiMGI:3027390. Lrrc4b.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000118831.
HOGENOMiHOG000252924.
HOVERGENiHBG052359.
InParanoidiP0C192.
KOiK16360.
OMAiTEGMGPG.
OrthoDBiEOG091G037N.
PhylomeDBiP0C192.
TreeFamiTF324303.

Miscellaneous databases

EvolutionaryTraceiP0C192.
PROiP0C192.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047085.
CleanExiMM_LRRC4B.
ExpressionAtlasiP0C192. baseline and differential.
GenevisibleiP0C192. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR026883. LRRC4B.
IPR000372. LRRNT.
[Graphical view]
PANTHERiPTHR24369:SF66. PTHR24369:SF66. 4 hits.
PfamiPF07679. I-set. 1 hit.
PF13855. LRR_8. 3 hits.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 8 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51450. LRR. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLRC4B_MOUSE
AccessioniPrimary (citable) accession number: P0C192
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: April 4, 2006
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.