P0C191 (GPVI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Platelet glycoprotein VI
Short name=GPVI

Alternative name(s):
Glycoprotein 5

Gene names

Name:

Gp6

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Collagen receptor involved in collagen-induced platelet adhesion and activation. Plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcR gamma-chain, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. Ref.1 Ref.3 Ref.4
Subunit structure	Associated with Fc receptor gamma chain. The GPVI-FcRgamma complex is associated with the Src kinase family Fyn and Lyn By similarity.
Subcellular location	Membrane; Single-pass membrane protein By similarity.
Tissue specificity	Megakaryocytes and platelets. Ref.1
Developmental stage	Expressed at embryonic day 13.5, 14.5 and 16.5. Expression decreases in intensity at day 18.5 and in 1.5 day-old newborn. Ref.1
Miscellaneous	Mice deficient in GPVI show a complete protection against arterial thrombosis and induced pulmonary thromboembolism, without significant prolongation of bleeding time.
Sequence similarities	Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Blood coagulation Hemostasis
Cellular component	Membrane
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to plasma membrane Inferred from direct assay Ref.1. Source: MGI
Molecular_function	collagen binding Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 313	292	Platelet glycoprotein VI		PRO_0000232384
Regions
	Topological domain	22 – 265	244	Extracellular Potential
	Transmembrane	266 – 286	21	Helical; Potential
	Topological domain	287 – 313	27	Cytoplasmic Potential
	Domain	27 – 105	79	Ig-like C2-type 1
	Domain	115 – 197	83	Ig-like C2-type 2
	Compositional bias	183 – 188	6	Poly-Ser
Amino acid modifications
	Glycosylation	93	1	N-linked (GlcNAc...) By similarity
	Glycosylation	244	1	N-linked (GlcNAc...) Potential
	Disulfide bond	49 ↔ 89		By similarity
	Disulfide bond	135 ↔ 181		By similarity
Experimental info
	Sequence conflict	256	1	F → I no nucleotide entry Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0C191 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 34D810EAE120C535	FASTA	313	34,595
	10 20 30 40 50 60 MSPASPTFFC IGLCVLQVIQ TQSGPLPKPS LQAQPSSLVP LGQSVILRCQ GPPDVDLYRL 70 80 90 100 110 120 EKLKPEKYED QDFLFIPTME RSNAGRYRCS YQNGSHWSLP SDQLELIATG VYAKPSLSAH 130 140 150 160 170 180 PSSAVPQGRD VTLKCQSPYS FDEFVLYKEG DTGSYKRPEK WYRANFPIIT VTAAHSGTYR 190 200 210 220 230 240 CYSFSSSSPY LWSAPSDPLV LVVTGLSATP SQVPTEESFP VTESSRRPSI LPTNKISTTE 250 260 270 280 290 300 KPMNITASPE GLSPPFGFAH QHYAKGNLVR ICLGATIIII LLGLLAEDWH SRKKCLQHRM 310 RALQRPLPPL PLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, characterization, and functional studies of human and mouse glycoprotein VI: a platelet-specific collagen receptor from the immunoglobulin superfamily." Jandrot-Perrus M., Busfield S., Lagrue A.-H., Xiong X., Debili N., Chickering T., Le Couedic J.-P., Goodearl A., Dussault B., Fraser C., Vainchenker W., Villeval J.-L. Blood 96:1798-1807(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH FC RECEPTOR GAMMA CHAIN, DEVELOPMENTAL STAGE. Tissue: Megakaryocyte.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The glycoprotein VI-phospholipase Cgamma2 signaling pathway controls thrombus formation induced by collagen and tissue factor in vitro and in vivo." Munnix I.C., Strehl A., Kuijpers M.J., Auger J.M., van der Meijden P.E., van Zandvoort M.A., oude Egbrink M.G., Nieswandt B., Heemskerk J.W. Arterioscler. Thromb. Vasc. Biol. 25:2673-2678(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"GPVI-deficient mice lack collagen responses and are protected against experimentally induced pulmonary thromboembolism." Lockyer S., Okuyama K., Begum S., Le S., Sun B., Watanabe T., Matsumoto Y., Yoshitake M., Kambayashi J., Tandon N.N. Thromb. Res. 118:371-380(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC137969 Genomic DNA. No translation available.
IPI	IPI00621831.
RefSeq	NP_001156486.1. NM_001163014.1.
UniGene	Mm.248662.
3D structure databases
ProteinModelPortal	P0C191.
SMR	P0C191. Positions 23-204.
ModBase	Search...
PTM databases
PhosphoSite	P0C191.
Proteomic databases
PaxDb	P0C191.
PRIDE	P0C191.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000108590; ENSMUSP00000104231; ENSMUSG00000078810.
GeneID	243816.
KEGG	mmu:243816.
UCSC	uc009exk.2. mouse.
Organism-specific databases
CTD	51206.
MGI	MGI:1889810. Gp6.
Phylogenomic databases
eggNOG	NOG79029.
GeneTree	ENSGT00700000104322.
HOGENOM	HOG000234395.
HOVERGEN	HBG074353.
InParanoid	P0C191.
KO	K06264.
OMA	HECESRR.
OrthoDB	EOG4D52ZF.
Gene expression databases
ArrayExpress	P0C191.
Bgee	P0C191.
Genevestigator	P0C191.
Family and domain databases
Gene3D	2.60.40.10. 2 hits.
InterPro	IPR013783. Ig-like_fold. IPR003599. Ig_sub. [Graphical view]
SMART	SM00409. IG. 2 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	385930.
SOURCE	Search...

Entry information

Entry name

GPVI_MOUSE

Accession

Primary (citable) accession number: P0C191
Secondary accession number(s): E9QPN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program