ID   PNTB_RHORU              Reviewed;         464 AA.
AC   P0C188; Q59763; Q59765;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   16-JUN-2009, entry version 25.
DE   RecName: Full=NAD(P) transhydrogenase subunit beta;
DE            EC=1.6.1.2;
DE   AltName: Full=Nicotinamide nucleotide transhydrogenase subunit beta;
DE   AltName: Full=Pyridine nucleotide transhydrogenase subunit beta;
DE   AltName: Full=Proton-translocating transhydrogenase NADP(H)-binding component;
DE   AltName: Full=dIII;
GN   Name=pntB; Synonyms=nntB;
OS   Rhodospirillum rubrum.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Rhodospirillum.
OX   NCBI_TaxID=1085;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=95146505; PubMed=7844118; DOI=10.1007/BF00762784;
RA   Yamaguchi M., Hatefi Y.;
RT   "Energy-transducing nicotinamide nucleotide transhydrogenase:
RT   nucleotide sequences of the genes and predicted amino acid sequences
RT   of the subunits of the enzyme from Rhodospirillum rubrum.";
RL   J. Bioenerg. Biomembr. 26:435-445(1994).
RN   [2]
RP   STRUCTURE BY NMR OF 262-464.
RX   MEDLINE=21061479; PubMed=11004437; DOI=10.1016/S0005-2728(00)00159-6;
RA   Jeeves M., Smith K.J., Quirk P.G., Cotton N.P.J., Jackson J.B.;
RT   "Solution structure of the NADP(H)-binding component (dIII) of proton-
RT   translocating transhydrogenase from Rhodospirillum rubrum.";
RL   Biochim. Biophys. Acta 1459:248-257(2000).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 262-464.
RX   MEDLINE=21340778; PubMed=11250201; DOI=10.1016/S0969-2126(01)00571-8;
RA   Cotton N.P.J., White S.A., Peake S.J., McSweeney S., Baz Jackson J.;
RT   "The crystal structure of an asymmetric complex of the two nucleotide
RT   binding components of proton-translocating transhydrogenase.";
RL   Structure 9:165-176(2001).
CC   -!- FUNCTION: The transhydrogenation between NADH and NADP is coupled
CC       to respiration and ATP hydrolysis and functions as a proton pump
CC       across the membrane (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADPH + NAD(+) = NADP(+) + NADH.
CC   -!- SUBUNIT: Complex of an alpha and a beta chain; in Rhodospirillum,
CC       the alpha chain seems to be made of two subunits.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane
CC       protein (By similarity).
CC   -!- SIMILARITY: Belongs to the PNT beta subunit family.
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DR   EMBL; U01158; AAC43257.1; -; Genomic_DNA.
DR   PDB; 2FR8; X-ray; 2.60 A; C=262-464.
DR   PDB; 2FRD; X-ray; 3.20 A; C=262-464.
DR   PDB; 2FSV; X-ray; 2.30 A; C=262-464.
DR   PDB; 2OO5; X-ray; 2.60 A; C=291-464.
DR   PDB; 2OOR; X-ray; 2.32 A; C=291-464.
DR   PDBsum; 2FR8; -.
DR   PDBsum; 2FRD; -.
DR   PDBsum; 2FSV; -.
DR   PDBsum; 2OO5; -.
DR   PDBsum; 2OOR; -.
DR   TCDB; 3.D.2.2.1; proton-translocating transhydrogenase (PTH) family.
DR   BRENDA; 1.6.1.2; 1671.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008750; F:NAD(P)+ transhydrogenase (AB-specific) acti...; IEA:EC.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; NAS:UniProtKB.
DR   InterPro; IPR012136; NADH_DH_b.
DR   InterPro; IPR004003; NADP_transhyd_b.
DR   Pfam; PF02233; PNTB; 1.
DR   PIRSF; PIRSF000204; PNTB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Membrane; NAD; NADP;
KW   Oxidoreductase; Transmembrane.
FT   CHAIN         1    464       NAD(P) transhydrogenase subunit beta.
FT                                /FTId=PRO_0000199027.
FT   TRANSMEM     54     74       Potential.
FT   TRANSMEM     86    106       Potential.
FT   TRANSMEM    126    146       Potential.
FT   TRANSMEM    164    184       Potential.
FT   TRANSMEM    191    211       Potential.
FT   TRANSMEM    227    247       Potential.
FT   HELIX       297    306
FT   STRAND      308    314
FT   HELIX       316    321
FT   HELIX       324    336
FT   STRAND      340    345
FT   STRAND      350    352
FT   HELIX       355    362
FT   HELIX       367    369
FT   STRAND      370    372
FT   HELIX       373    376
FT   HELIX       377    379
FT   STRAND      384    390
FT   HELIX       393    395
FT   HELIX       397    399
FT   TURN        406    409
FT   HELIX       415    417
FT   STRAND      418    428
FT   HELIX       438    441
FT   STRAND      445    450
FT   HELIX       452    462
SQ   SEQUENCE   464 AA;  47808 MW;  79200D4F8861779B CRC64;
     MTHSLTMAAY IVAGVLFILA LRGLSNPESA RNGNRMGMVG MAIAILTTLL SPSVQAYAWI
     VLAIAIGGAI GTVIAKKVLM TALPQLVAAF HSLVGMAAVL VATGALLNPE AYGIGSAGAI
     HAGSLVEMSL GLAVGAITFS GSVIAFGKLQ GLIAGKPVTF PMQHPLNAVL GILLVVLLVV
     FAATESHTAY FALMILAFAL GFLLIIPIGG ADMPVVISML NSYSGWAAAG IGFTLGNPLL
     IIAGALVGSS GAILSYIMCK GMNRSIFNVI LGGFGSEGGV AAAGGAAGDR SVKAGSAEDA
     AFIMKNASKV IIVPGYGMAV AQAQHALREM ADVLKKEGVE VSYAIHPVAG RMPGHMNVLL
     AEANVPYDEV FELEEINSSF QTADVAFVIG ANDVTNPAAK TDPSSPIYGM PILDVEKAGT
     VLFIKRSMAS GYAGVENELF FRNNTMMLFG DAKKMTEQIV QAMN
//