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Protein

Potassium channel toxin alpha-KTx 12.2

Gene
N/A
Organism
Tityus trivittatus (Argentinean scorpion)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits high conductance calcium-activated potassium channels (By similarity). Reversibly inhibits Shaker B potassium channels.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei30 – 301Basic residue of the functional dyadBy similarity
Sitei39 – 391Aromatic residue of the functional dyadBy similarity

GO - Molecular functioni

  1. ion channel inhibitor activity Source: InterPro

GO - Biological processi

  1. defense response Source: InterPro
  2. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Calcium-activated potassium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel toxin alpha-KTx 12.2
Alternative name(s):
Butantoxin
Short name:
BuTX
TtBut
OrganismiTityus trivittatus (Argentinean scorpion)
Taxonomic identifieri369776 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeTityus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4040Potassium channel toxin alpha-KTx 12.2PRO_0000226996Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi2 ↔ 5
Disulfide bondi10 ↔ 31
Disulfide bondi16 ↔ 36
Disulfide bondi20 ↔ 38

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

3D structure databases

SMRiP0C168. Positions 1-40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamiPF00451. Toxin_2. 1 hit.
[Graphical view]
PRINTSiPR00286. CHARYBDTOXIN.
ProDomiPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C168-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40
WCSTCLDLAC GASRECYDPC FKAFGRAHGK CMNNKCRCYT
Length:40
Mass (Da):4,514
Last modified:March 6, 2006 - v1
Checksum:i79C47786312FC1BD
GO

Mass spectrometryi

Molecular mass is 4507.0 Da from positions 1 - 40. Determined by ESI. 1 Publication

Cross-referencesi

3D structure databases

SMRiP0C168. Positions 1-40.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamiPF00451. Toxin_2. 1 hit.
[Graphical view]
PRINTSiPR00286. CHARYBDTOXIN.
ProDomiPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Disulfide bridges and blockage of Shaker B K(+)-channels by another butantoxin peptide purified from the Argentinean scorpion Tityus trivittatus."
    Coronas F.V., de Roodt A.R., Olamendi-Portugal T., Zamudio F.Z., Batista C.V.F., Gomez-Lagunas F., Possani L.D.
    Toxicon 41:173-179(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, DISULFIDE BONDS, MASS SPECTROMETRY.
  2. Cited for: STRUCTURE BY NMR, DISULFIDE BONDS, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiKA122_TITTR
AccessioniPrimary (citable) accession number: P0C168
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2006
Last sequence update: March 6, 2006
Last modified: March 31, 2015
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

The N-terminal C2-C5 disulfide bridge unique to this toxin does not appear to confer stability to the protein.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Scorpion potassium channel toxins
    Nomenclature of scorpion potassium channel toxins and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.