Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P0C168 (KA122_TITTR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium channel toxin alpha-KTx 12.2
Alternative name(s):
Butantoxin
Short name=BuTX
TtBut
OrganismTityus trivittatus (Argentinean scorpion)
Taxonomic identifier369776 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeTityus

Protein attributes

Sequence length40 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits high conductance calcium-activated potassium channels By similarity. Reversibly inhibits Shaker B potassium channels.

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Miscellaneous

The N-terminal C2-C5 disulfide bridge unique to this toxin does not appear to confer stability to the protein.

Sequence similarities

Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 12 subfamily.

Mass spectrometry

Molecular mass is 4507.0 Da from positions 1 - 40. Determined by ESI. Ref.1

Ontologies

Keywords
   Cellular componentSecreted
   Molecular functionIon channel impairing toxin
Neurotoxin
Potassium channel impairing toxin
Toxin
   PTMDisulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processdefense response

Inferred from electronic annotation. Source: InterPro

pathogenesis

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionion channel inhibitor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4040Potassium channel toxin alpha-KTx 12.2
PRO_0000226996

Sites

Site301Basic residue of the functional dyad By similarity
Site391Aromatic residue of the functional dyad By similarity

Amino acid modifications

Disulfide bond2 ↔ 5 Ref.1 Ref.2
Disulfide bond10 ↔ 31 Ref.1 Ref.2
Disulfide bond16 ↔ 36 Ref.1 Ref.2
Disulfide bond20 ↔ 38 Ref.1 Ref.2

Sequences

Sequence LengthMass (Da)Tools
P0C168 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 79C47786312FC1BD

FASTA404,514
        10         20         30         40 
WCSTCLDLAC GASRECYDPC FKAFGRAHGK CMNNKCRCYT 

« Hide

References

[1]"Disulfide bridges and blockage of Shaker B K(+)-channels by another butantoxin peptide purified from the Argentinean scorpion Tityus trivittatus."
Coronas F.V., de Roodt A.R., Olamendi-Portugal T., Zamudio F.Z., Batista C.V.F., Gomez-Lagunas F., Possani L.D.
Toxicon 41:173-179(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, DISULFIDE BONDS, MASS SPECTROMETRY.
[2]"NMR solution structure of butantoxin."
Holaday S.K. Jr., Martin B.M., Fletcher P.L. Jr., Krishna N.R.
Arch. Biochem. Biophys. 379:18-27(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR, DISULFIDE BONDS, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

3D structure databases

ProteinModelPortalP0C168.
SMRP0C168. Positions 1-40.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.30.10. 1 hit.
InterProIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamPF00451. Toxin_2. 1 hit.
[Graphical view]
PRINTSPR00286. CHARYBDTOXIN.
ProDomPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF57095. SSF57095. 1 hit.
PROSITEPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKA122_TITTR
AccessionPrimary (citable) accession number: P0C168
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 7, 2006
Last modified: April 16, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Scorpion potassium channel toxins

Nomenclature of scorpion potassium channel toxins and list of entries