Reviewed, UniProtKB/Swiss-Prot P0C166 (KAX6C_ANUPH)

Names and origin

Protein names	Recommended name:     Potassium channel toxin alpha-KTx 6.12 Alternative name(s):     Anuroctoxin
Organism	Anuroctonus phaiodactylus (Mafia scorpion)
Taxonomic identifier	246982 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Iurida › Chactoidea › Chactidae › Uroctoninae › Anuroctonus

Protein attributes

Sequence length	35 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	High affinity blocker of Kv1.3 (KCNA3) channels of human T cells. Does not block the calcium-activated IKCa1 potassium channels. Does not inhibit Shaker IR, mKv1.1, and rKv2.1 potassium channels. Blocks hKv1.2 with an order of magnitude smaller than for Kv1.3. Ref.1
Subunit structure	Monomer. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 6 subfamily.
Mass spectrometry	Molecular mass is 4082.8 Da from positions 1 - 35. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ionic channel inhibitor Neurotoxin Potassium channel inhibitor Toxin
PTM	Amidation Disulfide bond Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 35	35	Potassium channel toxin alpha-KTx 6.12		PRO_0000226963
Amino acid modifications
	Modified residue	1	1	Pyrrolidone carboxylic acid
	Modified residue	35	1	Lysine amide
	Disulfide bond	4 ↔ 24		Ref.1
	Disulfide bond	10 ↔ 29		Ref.1
	Disulfide bond	14 ↔ 31		Ref.1
	Disulfide bond	19 ↔ 34		Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0C166-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: B9B8E9CD7E4DA845	FASTA	35	4,109
	10 20 30 QKECTGPQHC TNFCRKNKCT HGKCMNRKCK CFNCK

References

[1]

"Anuroctoxin, a new scorpion toxin of the alpha-KTx 6 subfamily, is highly selective for Kv1.3 over IKCa1 ion channels of human T lymphocytes." Bagdany M., Batista C.V.F., Valdez-Cruz N.A., Somodi S., Rodriguez de la Vega R.C., Licea A.F., Varga Z., Gaspar R., Possani L.D., Panyi G. Mol. Pharmacol. 67:1034-1044(2005) [PubMed: 15615696] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBUNIT, DISULFIDE BONDS, MASS SPECTROMETRY. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001947. Scorpion_toxinS. [Graphical view]
Pfam	PF00451. Toxin_2. 1 hit. [Graphical view]
PROSITE	PS01138. SCORP_SHORT_TOXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAX6C_ANUPH

Accession

Primary (citable) accession number: P0C166

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	November 24, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)