P0C166 (KAX6C_ANUPH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin alpha-KTx 6.12 Alternative name(s): Anuroctoxin
Organism	Anuroctonus phaiodactylus (Mafia scorpion)
Taxonomic identifier	246982 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Iurida › Chactoidea › Chactidae › Uroctoninae › Anuroctonus

Protein attributes

Sequence length	35 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High affinity blocker of Kv1.3/KCNA3 channels of human T cells. Blocks Kv1.2/KCNA2 with an order of magnitude smaller than for Kv1.3/KCNA3. Ref.1
Subunit structure	Monomer. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Miscellaneous	This toxin does not block the calcium-activated KCa3.1/KCNN4 potassium channels, Shaker IR, Kv1.1/KCNA1, and Kv2.1/KCNB1 potassium channels (Ref.1).
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 6 subfamily.
Mass spectrometry	Molecular mass is 4082.8 Da from positions 1 - 35. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Potassium channel inhibitor Toxin
PTM	Amidation Disulfide bond Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 35	35	Potassium channel toxin alpha-KTx 6.12		PRO_0000226963
Sites
	Site	23	1	Basic residue of the functional dyad By similarity
	Site	32	1	Aromatic residue of the functional dyad By similarity
Amino acid modifications
	Modified residue	1	1	Pyrrolidone carboxylic acid
	Modified residue	35	1	Lysine amide
	Disulfide bond	4 ↔ 24		Ref.1
	Disulfide bond	10 ↔ 29		Ref.1
	Disulfide bond	14 ↔ 31		Ref.1
	Disulfide bond	19 ↔ 34		Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0C166 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: B9B8E9CD7E4DA845	FASTA	35	4,109
	10 20 30 QKECTGPQHC TNFCRKNKCT HGKCMNRKCK CFNCK

References

[1]

"Anuroctoxin, a new scorpion toxin of the alpha-KTx 6 subfamily, is highly selective for Kv1.3 over IKCa1 ion channels of human T lymphocytes." Bagdany M., Batista C.V.F., Valdez-Cruz N.A., Somodi S., Rodriguez de la Vega R.C., Licea A.F., Varga Z., Gaspar R., Possani L.D., Panyi G. Mol. Pharmacol. 67:1034-1044(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBUNIT, DISULFIDE BONDS, MASS SPECTROMETRY. Tissue: Venom.

Cross-references

3D structure databases
ProteinModelPortal	P0C166.
SMR	P0C166. Positions 3-34.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.30.10. 1 hit.
InterPro	IPR003614. Scorpion_toxin-like. IPR001947. Scorpion_toxinS_K_inh. [Graphical view]
Pfam	PF00451. Toxin_2. 1 hit. [Graphical view]
PROSITE	PS01138. SCORP_SHORT_TOXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAX6C_ANUPH

Accession

Primary (citable) accession number: P0C166

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	May 1, 2013
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program