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P0C124

- SYI_BRUA2

UniProt

P0C124 - SYI_BRUA2

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Protein
Isoleucine--tRNA ligase
Gene
ileS, BAB2_0194
Organism
Brucella abortus (strain 2308)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei603 – 6031Aminoacyl-adenylate By similarity
Binding sitei647 – 6471ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBABO359391:GKDV-2437-MONOMER.
BMEL359391:GJOQ-2437-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BAB2_0194
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719: Chromosome II

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 972972Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098362Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi359391.BAB2_0194.

Structurei

3D structure databases

ProteinModelPortaliP0C124.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi63 – 7311"HIGH" regionUniRule annotation
Add
BLAST
Motifi644 – 6485"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C124-1 [UniParc]FASTAAdd to Basket

« Hide

MTDTTKIDYS KTLYLPQTEF PMRAGLPQRE PLFVQRWEEM NLYKKLREQA    50
KDRPLYVLHD GPPYANGNIH IGHALNKILK DVITRSFQMR GYNSNYVPGW 100
DCHGLPIEWK IEEKYRAAGK NKDEVPINEF RKECREFASN WIKVQTEEFK 150
RLAILGDFEN PYTTMNFHAE ARIAGELLKF AASGQLYRGS KPVMWSVVER 200
TALAEAEVEY HDIESDMIWV KFPVAGEVAT ENDLSGSAVV IWTTTPWTIP 250
GNRAVSYSSR IEYGLFEITE AENDFGPRPG ERLVFADKLV EECCAKAKLQ 300
FKRLRSVSAE ELGKIVLDHP LKGFGGGYEF VVPMLDGDHV TDDAGTGFVH 350
TAPSHGREDF EAWMDNARQL EARGIDPNIP FPVGDDGFYT KDAPGFGPDR 400
EGGPARVIDD NGKKGDANKV VIEQLIAADK LFARGRLKHS YPHSWRSKKP 450
VIFRNTPQWF VYMDKNLGDG TTLRSRALKA IDETRFVPAA GQTRLRSMIE 500
GRPDWVLSRQ RAWGVPICVF VDEEGNILQD DAVNKRIMDA FEKEGADAWF 550
ADGARERFLG ARAGEGWTQV RDILDVWFDS GSTHTFTLED RPDLKWPADV 600
YLEGSDQHRG WFHSSLLESC GTRGRAPYNA VVTHGFTMDE HGKKMSKSLG 650
NTVTPQDVIK ESGADILRLW VMTTDYWEDQ RLGKSIIQTN IDAYRKLRNT 700
IRWMLGTLAH DEGENVAYAD LPELERLMLH RLTELDELVR SGYDTFDFKR 750
IARALVDFMN VELSAFYFDI RKDALYCDAP SSIRRKAALQ TVREIFVRLT 800
TWLAPMLPFT MEEAWLDRYP QSVSIHAEQF RPTPAEWRDD VLAEKWRKVR 850
AVRRVVTGAL ELERADKRIG SSLEAAPVVY IADKSLSDSL EGLDFAEICI 900
TSGISVSDAA APEGAFTLGD VKGVAVVPER AKGEKCARSW RYTTDVGADP 950
EFPEVSARDA AALRELQALG KL 972
Length:972
Mass (Da):110,042
Last modified:February 7, 2006 - v1
Checksum:i439543DEDCC20013
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM040265 Genomic DNA. Translation: CAJ12360.1.
RefSeqiYP_418423.1. NC_007624.1.

Genome annotation databases

EnsemblBacteriaiCAJ12360; CAJ12360; BAB2_0194.
GeneIDi3827171.
KEGGibmf:BAB2_0194.
PATRICi17847892. VBIBruMel86222_2479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM040265 Genomic DNA. Translation: CAJ12360.1 .
RefSeqi YP_418423.1. NC_007624.1.

3D structure databases

ProteinModelPortali P0C124.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 359391.BAB2_0194.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ12360 ; CAJ12360 ; BAB2_0194 .
GeneIDi 3827171.
KEGGi bmf:BAB2_0194.
PATRICi 17847892. VBIBruMel86222_2479.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BABO359391:GKDV-2437-MONOMER.
BMEL359391:GJOQ-2437-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.

Entry informationi

Entry nameiSYI_BRUA2
AccessioniPrimary (citable) accession number: P0C124
Secondary accession number(s): Q579P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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