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P0C115

- PURA_BRUAB

UniProt

P0C115 - PURA_BRUAB

Protein

Adenylosuccinate synthetase

Gene

purA

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP By similarity.By similarity

    Catalytic activityi

    GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei13 – 131Proton acceptorUniRule annotation
    Metal bindingi13 – 131MagnesiumUniRule annotation
    Metal bindingi40 – 401Magnesium; via carbonyl oxygenUniRule annotation
    Active sitei41 – 411Proton donorUniRule annotation
    Binding sitei129 – 1291IMPUniRule annotation
    Binding sitei143 – 1431IMP; shared with dimeric partnerUniRule annotation
    Binding sitei223 – 2231IMPUniRule annotation
    Binding sitei238 – 2381IMPUniRule annotation
    Binding sitei302 – 3021IMPUniRule annotation
    Binding sitei304 – 3041GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 187GTPUniRule annotation
    Nucleotide bindingi40 – 423GTPUniRule annotation
    Nucleotide bindingi330 – 3323GTPUniRule annotation
    Nucleotide bindingi412 – 4143GTPUniRule annotation

    GO - Molecular functioni

    1. adenylosuccinate synthase activity Source: UniProtKB-HAMAP
    2. GTP binding Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' AMP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBABO262698:GJC2-1708-MONOMER.
    UniPathwayiUPA00075; UER00335.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylosuccinate synthetaseUniRule annotation (EC:6.3.4.4UniRule annotation)
    Short name:
    AMPSaseUniRule annotation
    Short name:
    AdSSUniRule annotation
    Alternative name(s):
    IMP--aspartate ligaseUniRule annotation
    Gene namesi
    Name:purAUniRule annotation
    Ordered Locus Names:BruAb1_1668
    OrganismiBrucella abortus biovar 1 (strain 9-941)
    Taxonomic identifieri262698 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000540: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 429429Adenylosuccinate synthetasePRO_0000095153Add
    BLAST

    Proteomic databases

    PRIDEiP0C115.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi262698.BruAb1_1668.

    Structurei

    3D structure databases

    ProteinModelPortaliP0C115.
    SMRiP0C115. Positions 2-428.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni13 – 164IMP bindingUniRule annotation
    Regioni38 – 414IMP bindingUniRule annotation
    Regioni298 – 3047Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the adenylosuccinate synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0104.
    HOGENOMiHOG000260959.
    KOiK01939.
    OMAiITTGAER.
    OrthoDBiEOG68Q0QG.

    Family and domain databases

    HAMAPiMF_00011. Adenylosucc_synth.
    InterProiIPR018220. Adenylosuccinate_synthase_AS.
    IPR001114. Adenylosuccinate_synthetase.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11846. PTHR11846. 1 hit.
    PfamiPF00709. Adenylsucc_synt. 1 hit.
    [Graphical view]
    SMARTiSM00788. Adenylsucc_synt. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00184. purA. 1 hit.
    PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
    PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0C115-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANVVVVGSQ WGDEGKGKIV DWLSERADVI VRYQGGHNAG HTLVIDGVSY    50
    KLSLLPSGLV RGKLSVIGNG VVVDPHHFVA EVEKLRGQGI DVTPDVLRVA 100
    ENAPLILSIH RELDAMREGS NSGLKIGTTK RGIGPAYEDK VGRRAIRVID 150
    LTEPETLRPK VERLLAHHNS LRRGMGLEEI AVETILTELT SVADQILPYI 200
    DQVWRVLDER RKAGARILFE GAQGALLDND HGTYPFVTSS NTVAGQAAAG 250
    SGLGPTAIGY VLGITKAYTT RVGEGPFPTE LNDEIGEFLG TKGHEFGVVT 300
    GRKRRCGWFD AVIVRQTVRT SGINGIALTK LDVLDGLEEI KICVAYELDG 350
    KRIDYLPSSM GAQARVKPIY ETLPGWSETT AGARSWNDLP AQAVKYVRHI 400
    EELIGAPVAM LSTSPEREDT ILVTDPFHD 429
    Length:429
    Mass (Da):46,561
    Last modified:February 7, 2006 - v1
    Checksum:iDF90E83E30690FF2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017223 Genomic DNA. Translation: AAX74987.1.
    RefSeqiYP_222348.1. NC_006932.1.

    Genome annotation databases

    EnsemblBacteriaiAAX74987; AAX74987; BruAb1_1668.
    GeneIDi3340306.
    KEGGibmb:BruAb1_1668.
    PATRICi17825118. VBIBruAbo15061_1761.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017223 Genomic DNA. Translation: AAX74987.1 .
    RefSeqi YP_222348.1. NC_006932.1.

    3D structure databases

    ProteinModelPortali P0C115.
    SMRi P0C115. Positions 2-428.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 262698.BruAb1_1668.

    Proteomic databases

    PRIDEi P0C115.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAX74987 ; AAX74987 ; BruAb1_1668 .
    GeneIDi 3340306.
    KEGGi bmb:BruAb1_1668.
    PATRICi 17825118. VBIBruAbo15061_1761.

    Phylogenomic databases

    eggNOGi COG0104.
    HOGENOMi HOG000260959.
    KOi K01939.
    OMAi ITTGAER.
    OrthoDBi EOG68Q0QG.

    Enzyme and pathway databases

    UniPathwayi UPA00075 ; UER00335 .
    BioCyci BABO262698:GJC2-1708-MONOMER.

    Family and domain databases

    HAMAPi MF_00011. Adenylosucc_synth.
    InterProi IPR018220. Adenylosuccinate_synthase_AS.
    IPR001114. Adenylosuccinate_synthetase.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11846. PTHR11846. 1 hit.
    Pfami PF00709. Adenylsucc_synt. 1 hit.
    [Graphical view ]
    SMARTi SM00788. Adenylsucc_synt. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00184. purA. 1 hit.
    PROSITEi PS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
    PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
      Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
      J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 9-941.

    Entry informationi

    Entry nameiPURA_BRUAB
    AccessioniPrimary (citable) accession number: P0C115
    Secondary accession number(s): P52004, Q57BJ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella abortus strain 9-941
      Brucella abortus (strain 9-941): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3