P0C114 (DEGPL_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable periplasmic serine endoprotease DegP-like
EC=3.4.21.107

Alternative name(s):
Protease Do

Gene names

Name:	htrA
Ordered Locus Names:	BruAb1_0630

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Might be efficient in the degradation of transiently denatured and unfolded proteins which accumulate in the periplasm following stress conditions By similarity.
Catalytic activity	Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val.
Subcellular location	Periplasm Potential.
Sequence similarities	Belongs to the peptidase S1B family. Contains 2 PDZ (DHR) domains.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Periplasm
Domain	Repeat Signal
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	serine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 513	488	Probable periplasmic serine endoprotease DegP-like		PRO_0000026925
Regions
	Domain	300 – 391	92	PDZ 1
	Domain	414 – 500	87	PDZ 2
	Region	125 – 299	175	Serine protease
	Region	255 – 257	3	Substrate binding By similarity
	Region	312 – 316	5	Substrate binding By similarity
Sites
	Active site	152	1	Charge relay system Potential
	Active site	182	1	Charge relay system Potential
	Active site	257	1	Charge relay system Potential

Sequences

	Sequence		Length	Mass (Da)
	P0C114 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: DE1CEF1959472806	FASTA	513	53,483
	10 20 30 40 50 60 MSRARISNYR KGVAAVALSA ALAGAFVVTG PLGALNEARA EAVHVTPPPQ AGFADLVEKV 70 80 90 100 110 120 RPAVVSVRVK KDVQETSNRG PQFFGPPGFD QLPDGHPLKR FFRDFGMEPR GDSRSDNRRG 130 140 150 160 170 180 KANKPRPGHE RPVAQGSGFV ISEDGYVVTN NHVVSDGDAY TVVLDDGTEL DAKLIGADPR 190 200 210 220 230 240 TDLAVLKINA PKRKFVYVAF GDDNKVRVGD WVVAVGNPFG LGGTVTSGIV SARGRDIGAG 250 260 270 280 290 300 PYDDFIQIDA AVNKGNSGGP AFDLSGEVIG INTAIFSPSG GSVGIAFAIP SSTAKQVVDQ 310 320 330 340 350 360 LIKKGSVERG WIGVQIQPVT KDIAASLGLA EEKGAIVASP QDDGPAAKAG IKAGDVITAV 370 380 390 400 410 420 NGETVQDPRD LARKVANIAP GEKAALTVWR KNKAEEINVT IAAMPNDKGK SGSQSNDNDG 430 440 450 460 470 480 GQGETLDSYG LTVVPSEDGK GVVVTDVDPD SDAADRGIRS GDVIVSVNNQ TVKTAGDINK 490 500 510 AITAAEKSGR KAVLLQLQSN DQSRFVALPI NQE

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX74005.1.
RefSeq	YP_221366.1. NC_006932.1.
3D structure databases
ProteinModelPortal	P0C114.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3339359.
GenomeReviews	Gene locus BruAb1_0630 in contig AE017223_GR.
KEGG	bmb:BruAb1_0630.
PATRIC	17822861. VBIBruAbo15061_0667.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG585708.
OMA	DEGGLES.
PhylomeDB	P0C114.
ProtClustDB	CLSK864818.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0630-MONOMER.
Family and domain databases
InterPro	IPR001478. PDZ/DHR/GLGF. IPR009003. Pept_cys/ser_Trypsin-like. IPR011782. Pept_S1C_Do. IPR001254. Peptidase_S1_S6. IPR001940. Peptidase_S1C. [Graphical view]
KO	K01362.
Pfam	PF00595. PDZ. 2 hits. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00834. PROTEASES2C.
SMART	SM00228. PDZ. 2 hits. [Graphical view]
SUPFAM	SSF50156. PDZ. 2 hits. SSF50494. Pept_Ser_Cys. 1 hit.
TIGRFAMs	TIGR02037. DegP_htrA_DO. 1 hit.
PROSITE	PS50106. PDZ. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DEGPL_BRUAB

Accession

Primary (citable) accession number: P0C114
Secondary accession number(s): P0A3Z6, Q44597, Q57EC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program