P0C111 (LPXD_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	BruAb1_1159

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus › Brucella abortus bv. 1

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP-Rule MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP-Rule MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP-Rule MF_00523
Subunit structure	Homotrimer By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	UDP-3-O-acylglucosamine N-acyltransferase HAMAP-Rule MF_00523		PRO_0000059652
Sites
	Active site	257	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C111 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: ECC1ED7ED029D01A	FASTA	351	36,357
	10 20 30 40 50 60 MADPIFFKPS RELTIGDVAD FTGASLRDPK LAPRSVERLA SLKDAGEGAL VFVEGKKNVS 70 80 90 100 110 120 SLVGLKAAGV LCTESLADSV PSGIAVLVSR HPHRDFSAVG RMLFPASVRP ESWLGETGIS 130 140 150 160 170 180 PAAFIHPTAQ IEDGATVEAG AVIGSGVTIG AGTLIAATAV IGQNCQIGRN SYIAPGVSVQ 190 200 210 220 230 240 CAFIGNNVSL HPGVRIGQDG FGYVPGAAGL DKVPQLGRVI IQDNVEIGAN TTVDRGSLDD 250 260 270 280 290 300 TVIGEGTKID NLVQIAHNVR IGRFCLVAAH CGISGSCVIG DQTMLGGRVG LADHLIIGSR 310 320 330 340 350 VQVAAASGVM NDIPDGERWG GIPARPIKQW FRDIANIRSI GQSRKDASSD E

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX74498.1.
RefSeq	YP_221859.1. NC_006932.1.
3D structure databases
ProteinModelPortal	P0C111.
ModBase	Search...
Protein-protein interaction databases
STRING	262698.BruAb1_1159.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAX74498; AAX74498; BruAb1_1159.
GeneID	3339833.
KEGG	bmb:BruAb1_1159.
PATRIC	17824006. VBIBruAbo15061_1225.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HOG000294340.
KO	K02536.
OMA	CLIAAQC.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	BABO262698:GJC2-1181-MONOMER.
UniPathway	UPA00973.
Family and domain databases
HAMAP	MF_00523. LpxD.
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_BRUAB

Accession

Primary (citable) accession number: P0C111
Secondary accession number(s): P0A3P6, Q44630, Q57CY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program