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P0C0Y9

- RCEM_RHOSH

UniProt

P0C0Y9 - RCEM_RHOSH

Protein

Reaction center protein M chain

Gene

pufM

Organism
Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi183 – 1831Magnesium (bacteriochlorophyll b axial ligand)
    Metal bindingi203 – 2031Magnesium (bacteriochlorophyll b axial ligand)
    Metal bindingi220 – 2201Iron
    Metal bindingi235 – 2351Iron
    Binding sitei253 – 2531Quinone A
    Metal bindingi267 – 2671Iron

    GO - Molecular functioni

    1. bacteriochlorophyll binding Source: UniProtKB-KW
    2. electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. photosynthetic electron transport in photosystem II Source: InterPro
    2. protein-chromophore linkage Source: UniProtKB-KW

    Keywords - Biological processi

    Electron transport, Photosynthesis, Transport

    Keywords - Ligandi

    Bacteriochlorophyll, Chlorophyll, Chromophore, Iron, Magnesium, Metal-binding

    Protein family/group databases

    TCDBi3.E.2.1.1. the photosynthetic reaction center (prc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Reaction center protein M chain
    Alternative name(s):
    Photosynthetic reaction center M subunit
    Gene namesi
    Name:pufM
    OrganismiRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
    Taxonomic identifieri1063 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane-derived chromatophore membrane Source: UniProtKB-SubCell
    3. plasma membrane light-harvesting complex Source: InterPro

    Keywords - Cellular componenti

    Membrane, Reaction center

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 308307Reaction center protein M chainPRO_0000090417Add
    BLAST

    Interactioni

    Subunit structurei

    Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).

    Protein-protein interaction databases

    DIPiDIP-60490N.

    Structurei

    Secondary structure

    1
    308
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 83
    Beta strandi10 – 145
    Beta strandi24 – 263
    Helixi27 – 293
    Helixi38 – 414
    Beta strandi46 – 483
    Helixi55 – 7824
    Turni79 – 813
    Helixi83 – 886
    Turni90 – 923
    Helixi100 – 1023
    Beta strandi104 – 1063
    Helixi110 – 1123
    Helixi114 – 14027
    Helixi146 – 16217
    Helixi164 – 1696
    Helixi172 – 1743
    Helixi180 – 19314
    Helixi197 – 1993
    Helixi201 – 22626
    Helixi228 – 2303
    Turni231 – 2333
    Helixi235 – 2406
    Helixi244 – 25714
    Helixi265 – 28622
    Turni289 – 2913
    Beta strandi292 – 2943
    Helixi295 – 3017
    Beta strandi302 – 3065

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AIGX-ray2.60M/O2-308[»]
    1AIJX-ray2.20M/S2-308[»]
    1DS8X-ray2.50M/S2-307[»]
    1DV3X-ray2.50M/S2-307[»]
    1DV6X-ray2.50M/S2-307[»]
    1E14X-ray2.70M2-308[»]
    1E6DX-ray2.30M2-308[»]
    1F6NX-ray2.80M2-308[»]
    1FNPX-ray2.60M2-308[»]
    1FNQX-ray2.60M2-308[»]
    1JGWX-ray2.80M2-308[»]
    1JGXX-ray3.01M2-308[»]
    1JGYX-ray2.70M2-308[»]
    1JGZX-ray2.70M2-308[»]
    1JH0X-ray3.50M2-308[»]
    1K6LX-ray3.10M2-308[»]
    1K6NX-ray3.10M2-308[»]
    1KBYX-ray2.50M2-308[»]
    1L9BX-ray2.40M2-308[»]
    1L9JX-ray3.25M/S2-308[»]
    1M3XX-ray2.55M2-308[»]
    1MPSX-ray2.55M2-308[»]
    1OGVX-ray2.35M2-308[»]
    1PCRX-ray2.65M2-308[»]
    1PSSX-ray3.00M7-302[»]
    1PSTX-ray3.00M7-302[»]
    1QOVX-ray2.10M2-308[»]
    1RG5X-ray2.50M2-308[»]
    1RGNX-ray2.80M2-308[»]
    1RQKX-ray2.70M2-308[»]
    1RVJX-ray2.75M2-307[»]
    1RY5X-ray2.10M2-308[»]
    1RZHX-ray1.80M2-308[»]
    1RZZX-ray2.40M/S2-308[»]
    1S00X-ray2.60M/S2-308[»]
    1UMXX-ray2.80M2-308[»]
    1YF6X-ray2.25M2-308[»]
    1YSTX-ray3.00M2-306[»]
    1Z9JX-ray4.50B2-308[»]
    1Z9KX-ray4.60B2-308[»]
    2BNPX-ray2.70B2-308[»]
    2BNSX-ray2.50B2-308[»]
    2BOZX-ray2.40M2-308[»]
    2GMRX-ray2.50M2-308[»]
    2GNUX-ray2.20M3-302[»]
    2HG3X-ray2.70M2-308[»]
    2HG9X-ray2.45M2-308[»]
    2HH1X-ray2.55M2-308[»]
    2HHKX-ray2.50M2-308[»]
    2HITX-ray2.75M2-308[»]
    2HJ6X-ray3.00M2-308[»]
    2J8CX-ray1.87M2-308[»]
    2J8DX-ray2.07M2-308[»]
    2JIYX-ray2.20M1-308[»]
    2JJ0X-ray2.80M2-308[»]
    2RCRX-ray3.10M2-308[»]
    2UWSX-ray2.90M2-308[»]
    2UWTX-ray2.50M2-308[»]
    2UWUX-ray2.04M2-308[»]
    2UWVX-ray2.13M2-308[»]
    2UWWX-ray2.05M2-308[»]
    2UX3X-ray2.50M2-308[»]
    2UX4X-ray2.51M2-308[»]
    2UX5X-ray2.21M2-308[»]
    2UXJX-ray2.25M2-308[»]
    2UXKX-ray2.31M2-308[»]
    2UXLX-ray2.88M2-308[»]
    2UXMX-ray2.70M2-308[»]
    3DSYX-ray3.00M2-308[»]
    3DTAX-ray3.20M2-308[»]
    3DTRX-ray3.10M2-308[»]
    3DTSX-ray3.10M2-308[»]
    3DU2X-ray3.10M2-308[»]
    3DU3X-ray2.80M2-308[»]
    3DUQX-ray2.70M2-308[»]
    3I4DX-ray2.01M2-308[»]
    3V3YX-ray2.80M2-303[»]
    3V3ZX-ray2.90M2-303[»]
    3ZUMX-ray2.50M2-308[»]
    3ZUWX-ray2.31M2-308[»]
    4H99X-ray2.97M2-303[»]
    4H9LX-ray2.77M2-303[»]
    4HBHX-ray2.93M2-303[»]
    4HBJX-ray2.74M2-303[»]
    4IN7X-ray2.85M2-303[»]
    4JC9X-ray7.78M1-308[»]
    4JCBX-ray7.78M1-308[»]
    4N7KX-ray2.85M2-304[»]
    4RCRX-ray2.80M2-308[»]
    ProteinModelPortaliP0C0Y9.
    SMRiP0C0Y9. Positions 2-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0C0Y9.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei54 – 8027HelicalAdd
    BLAST
    Transmembranei111 – 14030HelicalAdd
    BLAST
    Transmembranei143 – 16826HelicalAdd
    BLAST
    Transmembranei198 – 22629HelicalAdd
    BLAST
    Transmembranei260 – 28627HelicalAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.20.85.10. 2 hits.
    InterProiIPR000484. Photo_RC_L/M.
    IPR005781. Photo_RC_M.
    [Graphical view]
    PfamiPF00124. Photo_RC. 1 hit.
    [Graphical view]
    PRINTSiPR00256. REACTNCENTRE.
    SUPFAMiSSF81483. SSF81483. 1 hit.
    TIGRFAMsiTIGR01115. pufM. 1 hit.
    PROSITEiPS00244. REACTION_CENTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0C0Y9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEYQNIFSQ VQVRGPADLG MTEDVNLANR SGVGPFSTLL GWFGNAQLGP    50
    IYLGSLGVLS LFSGLMWFFT IGIWFWYQAG WNPAVFLRDL FFFSLEPPAP 100
    EYGLSFAAPL KEGGLWLIAS FFMFVAVWSW WGRTYLRAQA LGMGKHTAWA 150
    FLSAIWLWMV LGFIRPILMG SWSEAVPYGI FSHLDWTNNF SLVHGNLFYN 200
    PFHGLSIAFL YGSALLFAMH GATILAVSRF GGERELEQIA DRGTAAERAA 250
    LFWRWTMGFN ATMEGIHRWA IWMAVLVTLT GGIGILLSGT VVDNWYVWGQ 300
    NHGMAPLN 308
    Length:308
    Mass (Da):34,509
    Last modified:January 23, 2007 - v2
    Checksum:i30B3737DF8658250
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti141 – 1411L → M in CAA45001. (PubMed:2126457)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00827 Genomic DNA. Translation: AAA26179.1.
    X63405 Genomic DNA. Translation: CAA45001.1.
    X63404 Genomic DNA. Translation: CAA45000.1.
    M10206 Genomic DNA. Translation: AAA26178.1.
    PIRiA03456. WNRFMS.
    S24213.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00827 Genomic DNA. Translation: AAA26179.1 .
    X63405 Genomic DNA. Translation: CAA45001.1 .
    X63404 Genomic DNA. Translation: CAA45000.1 .
    M10206 Genomic DNA. Translation: AAA26178.1 .
    PIRi A03456. WNRFMS.
    S24213.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AIG X-ray 2.60 M/O 2-308 [» ]
    1AIJ X-ray 2.20 M/S 2-308 [» ]
    1DS8 X-ray 2.50 M/S 2-307 [» ]
    1DV3 X-ray 2.50 M/S 2-307 [» ]
    1DV6 X-ray 2.50 M/S 2-307 [» ]
    1E14 X-ray 2.70 M 2-308 [» ]
    1E6D X-ray 2.30 M 2-308 [» ]
    1F6N X-ray 2.80 M 2-308 [» ]
    1FNP X-ray 2.60 M 2-308 [» ]
    1FNQ X-ray 2.60 M 2-308 [» ]
    1JGW X-ray 2.80 M 2-308 [» ]
    1JGX X-ray 3.01 M 2-308 [» ]
    1JGY X-ray 2.70 M 2-308 [» ]
    1JGZ X-ray 2.70 M 2-308 [» ]
    1JH0 X-ray 3.50 M 2-308 [» ]
    1K6L X-ray 3.10 M 2-308 [» ]
    1K6N X-ray 3.10 M 2-308 [» ]
    1KBY X-ray 2.50 M 2-308 [» ]
    1L9B X-ray 2.40 M 2-308 [» ]
    1L9J X-ray 3.25 M/S 2-308 [» ]
    1M3X X-ray 2.55 M 2-308 [» ]
    1MPS X-ray 2.55 M 2-308 [» ]
    1OGV X-ray 2.35 M 2-308 [» ]
    1PCR X-ray 2.65 M 2-308 [» ]
    1PSS X-ray 3.00 M 7-302 [» ]
    1PST X-ray 3.00 M 7-302 [» ]
    1QOV X-ray 2.10 M 2-308 [» ]
    1RG5 X-ray 2.50 M 2-308 [» ]
    1RGN X-ray 2.80 M 2-308 [» ]
    1RQK X-ray 2.70 M 2-308 [» ]
    1RVJ X-ray 2.75 M 2-307 [» ]
    1RY5 X-ray 2.10 M 2-308 [» ]
    1RZH X-ray 1.80 M 2-308 [» ]
    1RZZ X-ray 2.40 M/S 2-308 [» ]
    1S00 X-ray 2.60 M/S 2-308 [» ]
    1UMX X-ray 2.80 M 2-308 [» ]
    1YF6 X-ray 2.25 M 2-308 [» ]
    1YST X-ray 3.00 M 2-306 [» ]
    1Z9J X-ray 4.50 B 2-308 [» ]
    1Z9K X-ray 4.60 B 2-308 [» ]
    2BNP X-ray 2.70 B 2-308 [» ]
    2BNS X-ray 2.50 B 2-308 [» ]
    2BOZ X-ray 2.40 M 2-308 [» ]
    2GMR X-ray 2.50 M 2-308 [» ]
    2GNU X-ray 2.20 M 3-302 [» ]
    2HG3 X-ray 2.70 M 2-308 [» ]
    2HG9 X-ray 2.45 M 2-308 [» ]
    2HH1 X-ray 2.55 M 2-308 [» ]
    2HHK X-ray 2.50 M 2-308 [» ]
    2HIT X-ray 2.75 M 2-308 [» ]
    2HJ6 X-ray 3.00 M 2-308 [» ]
    2J8C X-ray 1.87 M 2-308 [» ]
    2J8D X-ray 2.07 M 2-308 [» ]
    2JIY X-ray 2.20 M 1-308 [» ]
    2JJ0 X-ray 2.80 M 2-308 [» ]
    2RCR X-ray 3.10 M 2-308 [» ]
    2UWS X-ray 2.90 M 2-308 [» ]
    2UWT X-ray 2.50 M 2-308 [» ]
    2UWU X-ray 2.04 M 2-308 [» ]
    2UWV X-ray 2.13 M 2-308 [» ]
    2UWW X-ray 2.05 M 2-308 [» ]
    2UX3 X-ray 2.50 M 2-308 [» ]
    2UX4 X-ray 2.51 M 2-308 [» ]
    2UX5 X-ray 2.21 M 2-308 [» ]
    2UXJ X-ray 2.25 M 2-308 [» ]
    2UXK X-ray 2.31 M 2-308 [» ]
    2UXL X-ray 2.88 M 2-308 [» ]
    2UXM X-ray 2.70 M 2-308 [» ]
    3DSY X-ray 3.00 M 2-308 [» ]
    3DTA X-ray 3.20 M 2-308 [» ]
    3DTR X-ray 3.10 M 2-308 [» ]
    3DTS X-ray 3.10 M 2-308 [» ]
    3DU2 X-ray 3.10 M 2-308 [» ]
    3DU3 X-ray 2.80 M 2-308 [» ]
    3DUQ X-ray 2.70 M 2-308 [» ]
    3I4D X-ray 2.01 M 2-308 [» ]
    3V3Y X-ray 2.80 M 2-303 [» ]
    3V3Z X-ray 2.90 M 2-303 [» ]
    3ZUM X-ray 2.50 M 2-308 [» ]
    3ZUW X-ray 2.31 M 2-308 [» ]
    4H99 X-ray 2.97 M 2-303 [» ]
    4H9L X-ray 2.77 M 2-303 [» ]
    4HBH X-ray 2.93 M 2-303 [» ]
    4HBJ X-ray 2.74 M 2-303 [» ]
    4IN7 X-ray 2.85 M 2-303 [» ]
    4JC9 X-ray 7.78 M 1-308 [» ]
    4JCB X-ray 7.78 M 1-308 [» ]
    4N7K X-ray 2.85 M 2-304 [» ]
    4RCR X-ray 2.80 M 2-308 [» ]
    ProteinModelPortali P0C0Y9.
    SMRi P0C0Y9. Positions 2-308.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60490N.

    Protein family/group databases

    TCDBi 3.E.2.1.1. the photosynthetic reaction center (prc) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P0C0Y9.

    Family and domain databases

    Gene3Di 1.20.85.10. 2 hits.
    InterProi IPR000484. Photo_RC_L/M.
    IPR005781. Photo_RC_M.
    [Graphical view ]
    Pfami PF00124. Photo_RC. 1 hit.
    [Graphical view ]
    PRINTSi PR00256. REACTNCENTRE.
    SUPFAMi SSF81483. SSF81483. 1 hit.
    TIGRFAMsi TIGR01115. pufM. 1 hit.
    PROSITEi PS00244. REACTION_CENTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the M subunit of the reaction center from Rhodopseudomonas sphaeroides."
      Williams J.C., Steiner L.A., Ogden R.C., Simon M.I., Feher G.
      Proc. Natl. Acad. Sci. U.S.A. 80:6505-6509(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Towards the understanding of the function of Rb sphaeroides Y wild type reaction center: gene cloning, protein and detergent structures in the three-dimensional crystals."
      Arnoux B., Ducruix A., Astier C., Picaud M., Roth M., Reiss-Husson F.
      Biochimie 72:525-530(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Y.
    3. "Primary structure of the L subunit of the reaction center from Rhodopseudomonas sphaeroides."
      Williams J.C., Steiner L.A., Feher G., Simon M.I.
      Proc. Natl. Acad. Sci. U.S.A. 81:7303-7307(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
    4. "Structure of the membrane-bound protein photosynthetic reaction center from Rhodobacter sphaeroides."
      Chang C.-H., El-Kabbani O., Tiede D., Norris J., Schiffer M.
      Biochemistry 30:5352-5360(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
    5. "Structure of the reaction center from Rhodobacter sphaeroides R-26: protein-cofactor (quinones and Fe2+) interactions."
      Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.
      Proc. Natl. Acad. Sci. U.S.A. 85:8487-8491(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
      Strain: R-26.
    6. "Structure of the reaction center from Rhodobacter sphaeroides R-26: the protein subunits."
      Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.
      Proc. Natl. Acad. Sci. U.S.A. 84:6162-6166(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
      Strain: R-26.
    7. "Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: monitoring the optical properties of the complex from bacterial cell to crystal."
      McAuley-Hecht K.E., Fyfe P.K., Ridge J.P., Prince S.M., Hunter C.N., Isaacs N.W., Cogdell R.J., Jones M.R.
      Biochemistry 37:4740-4750(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).

    Entry informationi

    Entry nameiRCEM_RHOSH
    AccessioniPrimary (citable) accession number: P0C0Y9
    Secondary accession number(s): P02953, Q9RFB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 62 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3