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P0C0Y9 (RCEM_RHOSH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Reaction center protein M chain
Alternative name(s):
Photosynthetic reaction center M subunit
Gene names
Name:pufM
OrganismRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Taxonomic identifier1063 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.

Subunit structure

Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).

Subcellular location

Cellular chromatophore membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the reaction center PufL/M/PsbA/D family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 308307Reaction center protein M chain
PRO_0000090417

Regions

Transmembrane54 – 8027Helical
Transmembrane111 – 14030Helical
Transmembrane143 – 16826Helical
Transmembrane198 – 22629Helical
Transmembrane260 – 28627Helical

Sites

Metal binding1831Magnesium (bacteriochlorophyll b axial ligand)
Metal binding2031Magnesium (bacteriochlorophyll b axial ligand)
Metal binding2201Iron
Metal binding2351Iron
Metal binding2671Iron
Binding site2531Quinone A

Experimental info

Sequence conflict1411L → M in CAA45001. Ref.2

Secondary structure

1 ..................................................... 308
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0C0Y9 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 30B3737DF8658250

FASTA30834,509
        10         20         30         40         50         60 
MAEYQNIFSQ VQVRGPADLG MTEDVNLANR SGVGPFSTLL GWFGNAQLGP IYLGSLGVLS 

        70         80         90        100        110        120 
LFSGLMWFFT IGIWFWYQAG WNPAVFLRDL FFFSLEPPAP EYGLSFAAPL KEGGLWLIAS 

       130        140        150        160        170        180 
FFMFVAVWSW WGRTYLRAQA LGMGKHTAWA FLSAIWLWMV LGFIRPILMG SWSEAVPYGI 

       190        200        210        220        230        240 
FSHLDWTNNF SLVHGNLFYN PFHGLSIAFL YGSALLFAMH GATILAVSRF GGERELEQIA 

       250        260        270        280        290        300 
DRGTAAERAA LFWRWTMGFN ATMEGIHRWA IWMAVLVTLT GGIGILLSGT VVDNWYVWGQ 


NHGMAPLN

« Hide

References

[1]"Primary structure of the M subunit of the reaction center from Rhodopseudomonas sphaeroides."
Williams J.C., Steiner L.A., Ogden R.C., Simon M.I., Feher G.
Proc. Natl. Acad. Sci. U.S.A. 80:6505-6509(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Towards the understanding of the function of Rb sphaeroides Y wild type reaction center: gene cloning, protein and detergent structures in the three-dimensional crystals."
Arnoux B., Ducruix A., Astier C., Picaud M., Roth M., Reiss-Husson F.
Biochimie 72:525-530(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Y.
[3]"Primary structure of the L subunit of the reaction center from Rhodopseudomonas sphaeroides."
Williams J.C., Steiner L.A., Feher G., Simon M.I.
Proc. Natl. Acad. Sci. U.S.A. 81:7303-7307(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
[4]"Structure of the membrane-bound protein photosynthetic reaction center from Rhodobacter sphaeroides."
Chang C.-H., El-Kabbani O., Tiede D., Norris J., Schiffer M.
Biochemistry 30:5352-5360(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
[5]"Structure of the reaction center from Rhodobacter sphaeroides R-26: protein-cofactor (quinones and Fe2+) interactions."
Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.
Proc. Natl. Acad. Sci. U.S.A. 85:8487-8491(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
Strain: R-26.
[6]"Structure of the reaction center from Rhodobacter sphaeroides R-26: the protein subunits."
Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.
Proc. Natl. Acad. Sci. U.S.A. 84:6162-6166(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
Strain: R-26.
[7]"Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: monitoring the optical properties of the complex from bacterial cell to crystal."
McAuley-Hecht K.E., Fyfe P.K., Ridge J.P., Prince S.M., Hunter C.N., Isaacs N.W., Cogdell R.J., Jones M.R.
Biochemistry 37:4740-4750(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		K00827 Genomic DNA. Translation: AAA26179.1.
X63405 Genomic DNA. Translation: CAA45001.1.
X63404 Genomic DNA. Translation: CAA45000.1.
M10206 Genomic DNA. Translation: AAA26178.1.
PIRWNRFMS. A03456.
S24213.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AIGX-ray2.60M/O2-308[»]
1AIJX-ray2.20M/S2-308[»]
1DS8X-ray2.50M/S2-307[»]
1DV3X-ray2.50M/S2-307[»]
1DV6X-ray2.50M/S2-307[»]
1E14X-ray2.70M2-308[»]
1E6DX-ray2.30M2-308[»]
1F6NX-ray2.80M2-308[»]
1FNPX-ray2.60M2-308[»]
1FNQX-ray2.60M2-308[»]
1JGWX-ray2.80M2-308[»]
1JGXX-ray3.01M2-308[»]
1JGYX-ray2.70M2-308[»]
1JGZX-ray2.70M2-308[»]
1JH0X-ray3.50M2-308[»]
1K6LX-ray3.10M2-308[»]
1K6NX-ray3.10M2-308[»]
1KBYX-ray2.50M2-308[»]
1L9BX-ray2.40M2-308[»]
1L9JX-ray3.25M/S2-308[»]
1M3XX-ray2.55M2-308[»]
1MPSX-ray2.55M2-308[»]
1OGVX-ray2.35M2-308[»]
1PCRX-ray2.65M2-308[»]
1PSSX-ray3.00M7-302[»]
1PSTX-ray3.00M7-302[»]
1QOVX-ray2.10M2-308[»]
1RG5X-ray2.50M2-308[»]
1RGNX-ray2.80M2-308[»]
1RQKX-ray2.70M2-308[»]
1RVJX-ray2.75M2-307[»]
1RY5X-ray2.10M2-308[»]
1RZHX-ray1.80M2-308[»]
1RZZX-ray2.40M/S2-308[»]
1S00X-ray2.60M/S2-308[»]
1UMXX-ray2.80M2-308[»]
1YF6X-ray2.25M2-308[»]
1YSTX-ray3.00M2-306[»]
1Z9JX-ray4.50B2-308[»]
1Z9KX-ray4.60B2-308[»]
2BNPX-ray2.70B2-308[»]
2BNSX-ray2.50B2-308[»]
2BOZX-ray2.40M2-308[»]
2GMRX-ray2.50M2-308[»]
2GNUX-ray2.20M3-302[»]
2HG3X-ray2.70M2-308[»]
2HG9X-ray2.45M2-308[»]
2HH1X-ray2.55M2-308[»]
2HHKX-ray2.50M2-308[»]
2HITX-ray2.75M2-308[»]
2HJ6X-ray3.00M2-308[»]
2J8CX-ray1.87M2-308[»]
2J8DX-ray2.07M2-308[»]
2JIYX-ray2.20M2-308[»]
2JJ0X-ray2.80M2-308[»]
2RCRX-ray3.10M2-308[»]
2UWSX-ray2.90M2-308[»]
2UWTX-ray2.50M2-308[»]
2UWUX-ray2.04M2-308[»]
2UWVX-ray2.13M2-308[»]
2UWWX-ray2.05M2-308[»]
2UX3X-ray2.50M2-308[»]
2UX4X-ray2.51M2-308[»]
2UX5X-ray2.21M2-308[»]
2UXJX-ray2.25M2-308[»]
2UXKX-ray2.31M2-308[»]
2UXLX-ray2.88M2-308[»]
2UXMX-ray2.70M2-308[»]
3DSYX-ray3.00M2-308[»]
3DTAX-ray3.20M2-308[»]
3DTRX-ray3.10M2-308[»]
3DTSX-ray3.10M2-308[»]
3DU2X-ray3.10M2-308[»]
3DU3X-ray2.80M2-308[»]
3DUQX-ray2.70M2-308[»]
3I4DX-ray2.01M2-308[»]
3V3YX-ray2.80M2-303[»]
3V3ZX-ray2.90M2-303[»]
3ZUMX-ray2.50M2-308[»]
3ZUWX-ray2.31M2-308[»]
4H99X-ray2.97M2-308[»]
4H9LX-ray2.77M2-308[»]
4HBHX-ray2.93M2-308[»]
4HBJX-ray2.74M2-308[»]
4RCRX-ray2.80M2-308[»]
ProteinModelPortalP0C0Y9.
SMRP0C0Y9. Positions 2-308.
ModBaseSearch...

Protein family/group databases

TCDB3.E.2.1.1. photosynthetic reaction center (PRC) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.85.10. 2 hits.
InterProIPR000484. Photo_RC_L/M.
IPR005781. Photo_RC_M.
[Graphical view]
PfamPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSPR00256. REACTNCENTRE.
SUPFAMSSF81483. Photo_RC_L/M. 1 hit.
TIGRFAMsTIGR01115. pufM. 1 hit.
PROSITEPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0C0Y9.

Entry information

Entry nameRCEM_RHOSH
AccessionPrimary (citable) accession number: P0C0Y9
Secondary accession number(s): P02953, Q9RFB8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 3, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents