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P0C0Y9

- RCEM_RHOSH

UniProt

P0C0Y9 - RCEM_RHOSH

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Protein

Reaction center protein M chain

Gene

pufM

Organism
Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi183 – 1831Magnesium (bacteriochlorophyll b axial ligand)
Metal bindingi203 – 2031Magnesium (bacteriochlorophyll b axial ligand)
Metal bindingi220 – 2201Iron
Metal bindingi235 – 2351Iron
Binding sitei253 – 2531Quinone A
Metal bindingi267 – 2671Iron

GO - Molecular functioni

  1. bacteriochlorophyll binding Source: UniProtKB-KW
  2. electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. photosynthetic electron transport in photosystem II Source: InterPro
  2. protein-chromophore linkage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Bacteriochlorophyll, Chlorophyll, Chromophore, Iron, Magnesium, Metal-binding

Protein family/group databases

TCDBi3.E.2.1.1. the photosynthetic reaction center (prc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Reaction center protein M chain
Alternative name(s):
Photosynthetic reaction center M subunit
Gene namesi
Name:pufM
OrganismiRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Taxonomic identifieri1063 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei54 – 8027HelicalAdd
BLAST
Transmembranei111 – 14030HelicalAdd
BLAST
Transmembranei143 – 16826HelicalAdd
BLAST
Transmembranei198 – 22629HelicalAdd
BLAST
Transmembranei260 – 28627HelicalAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane light-harvesting complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Reaction center

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 308307Reaction center protein M chainPRO_0000090417Add
BLAST

Interactioni

Subunit structurei

Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).

Protein-protein interaction databases

DIPiDIP-60490N.

Structurei

Secondary structure

1
308
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 83Combined sources
Beta strandi10 – 145Combined sources
Beta strandi24 – 263Combined sources
Helixi27 – 293Combined sources
Helixi38 – 414Combined sources
Beta strandi46 – 483Combined sources
Helixi55 – 7824Combined sources
Turni79 – 813Combined sources
Helixi83 – 886Combined sources
Turni90 – 923Combined sources
Helixi100 – 1023Combined sources
Beta strandi104 – 1063Combined sources
Helixi110 – 1123Combined sources
Helixi114 – 14027Combined sources
Helixi146 – 16217Combined sources
Helixi164 – 1696Combined sources
Helixi172 – 1743Combined sources
Helixi180 – 19314Combined sources
Helixi197 – 1993Combined sources
Helixi201 – 22626Combined sources
Helixi228 – 2303Combined sources
Turni231 – 2333Combined sources
Helixi235 – 2406Combined sources
Helixi244 – 25714Combined sources
Helixi265 – 28622Combined sources
Turni289 – 2913Combined sources
Beta strandi292 – 2943Combined sources
Helixi295 – 3017Combined sources
Beta strandi302 – 3065Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AIGX-ray2.60M/O2-308[»]
1AIJX-ray2.20M/S2-308[»]
1DS8X-ray2.50M/S2-307[»]
1DV3X-ray2.50M/S2-307[»]
1DV6X-ray2.50M/S2-307[»]
1E14X-ray2.70M2-308[»]
1E6DX-ray2.30M2-308[»]
1F6NX-ray2.80M2-308[»]
1FNPX-ray2.60M2-308[»]
1FNQX-ray2.60M2-308[»]
1JGWX-ray2.80M2-308[»]
1JGXX-ray3.01M2-308[»]
1JGYX-ray2.70M2-308[»]
1JGZX-ray2.70M2-308[»]
1JH0X-ray3.50M2-308[»]
1K6LX-ray3.10M2-308[»]
1K6NX-ray3.10M2-308[»]
1KBYX-ray2.50M2-308[»]
1L9BX-ray2.40M2-308[»]
1L9JX-ray3.25M/S2-308[»]
1M3XX-ray2.55M2-308[»]
1MPSX-ray2.55M2-308[»]
1OGVX-ray2.35M2-308[»]
1PCRX-ray2.65M2-308[»]
1PSSX-ray3.00M7-302[»]
1PSTX-ray3.00M7-302[»]
1QOVX-ray2.10M2-308[»]
1RG5X-ray2.50M2-308[»]
1RGNX-ray2.80M2-308[»]
1RQKX-ray2.70M2-308[»]
1RVJX-ray2.75M2-307[»]
1RY5X-ray2.10M2-308[»]
1RZHX-ray1.80M2-308[»]
1RZZX-ray2.40M/S2-308[»]
1S00X-ray2.60M/S2-308[»]
1UMXX-ray2.80M2-308[»]
1YF6X-ray2.25M2-308[»]
1YSTX-ray3.00M2-306[»]
1Z9JX-ray4.50B2-308[»]
1Z9KX-ray4.60B2-308[»]
2BNPX-ray2.70B2-308[»]
2BNSX-ray2.50B2-308[»]
2BOZX-ray2.40M2-308[»]
2GMRX-ray2.50M2-308[»]
2GNUX-ray2.20M3-302[»]
2HG3X-ray2.70M2-308[»]
2HG9X-ray2.45M2-308[»]
2HH1X-ray2.55M2-308[»]
2HHKX-ray2.50M2-308[»]
2HITX-ray2.75M2-308[»]
2HJ6X-ray3.00M2-308[»]
2J8CX-ray1.87M2-308[»]
2J8DX-ray2.07M2-308[»]
2JIYX-ray2.20M1-308[»]
2JJ0X-ray2.80M2-308[»]
2RCRX-ray3.10M2-308[»]
2UWSX-ray2.90M2-308[»]
2UWTX-ray2.50M2-308[»]
2UWUX-ray2.04M2-308[»]
2UWVX-ray2.13M2-308[»]
2UWWX-ray2.05M2-308[»]
2UX3X-ray2.50M2-308[»]
2UX4X-ray2.51M2-308[»]
2UX5X-ray2.21M2-308[»]
2UXJX-ray2.25M2-308[»]
2UXKX-ray2.31M2-308[»]
2UXLX-ray2.88M2-308[»]
2UXMX-ray2.70M2-308[»]
3DSYX-ray3.00M2-308[»]
3DTAX-ray3.20M2-308[»]
3DTRX-ray3.10M2-308[»]
3DTSX-ray3.10M2-308[»]
3DU2X-ray3.10M2-308[»]
3DU3X-ray2.80M2-308[»]
3DUQX-ray2.70M2-308[»]
3I4DX-ray2.01M2-308[»]
3V3YX-ray2.80M2-303[»]
3V3ZX-ray2.90M2-303[»]
3ZUMX-ray2.50M2-308[»]
3ZUWX-ray2.31M2-308[»]
4H99X-ray2.97M2-303[»]
4H9LX-ray2.77M2-303[»]
4HBHX-ray2.93M2-303[»]
4HBJX-ray2.74M2-303[»]
4IN7X-ray2.85M2-303[»]
4JC9X-ray7.78M1-308[»]
4JCBX-ray7.78M1-308[»]
4LWYX-ray2.90M1-303[»]
4N7KX-ray2.85M2-304[»]
4RCRX-ray2.80M2-308[»]
4TQQX-ray2.50M2-303[»]
ProteinModelPortaliP0C0Y9.
SMRiP0C0Y9. Positions 2-308.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0Y9.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.85.10. 2 hits.
InterProiIPR000484. Photo_RC_L/M.
IPR005781. Photo_RC_M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01115. pufM. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C0Y9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEYQNIFSQ VQVRGPADLG MTEDVNLANR SGVGPFSTLL GWFGNAQLGP
60 70 80 90 100
IYLGSLGVLS LFSGLMWFFT IGIWFWYQAG WNPAVFLRDL FFFSLEPPAP
110 120 130 140 150
EYGLSFAAPL KEGGLWLIAS FFMFVAVWSW WGRTYLRAQA LGMGKHTAWA
160 170 180 190 200
FLSAIWLWMV LGFIRPILMG SWSEAVPYGI FSHLDWTNNF SLVHGNLFYN
210 220 230 240 250
PFHGLSIAFL YGSALLFAMH GATILAVSRF GGERELEQIA DRGTAAERAA
260 270 280 290 300
LFWRWTMGFN ATMEGIHRWA IWMAVLVTLT GGIGILLSGT VVDNWYVWGQ

NHGMAPLN
Length:308
Mass (Da):34,509
Last modified:January 23, 2007 - v2
Checksum:i30B3737DF8658250
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti141 – 1411L → M in CAA45001. (PubMed:2126457)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00827 Genomic DNA. Translation: AAA26179.1.
X63405 Genomic DNA. Translation: CAA45001.1.
X63404 Genomic DNA. Translation: CAA45000.1.
M10206 Genomic DNA. Translation: AAA26178.1.
PIRiA03456. WNRFMS.
S24213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00827 Genomic DNA. Translation: AAA26179.1 .
X63405 Genomic DNA. Translation: CAA45001.1 .
X63404 Genomic DNA. Translation: CAA45000.1 .
M10206 Genomic DNA. Translation: AAA26178.1 .
PIRi A03456. WNRFMS.
S24213.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AIG X-ray 2.60 M/O 2-308 [» ]
1AIJ X-ray 2.20 M/S 2-308 [» ]
1DS8 X-ray 2.50 M/S 2-307 [» ]
1DV3 X-ray 2.50 M/S 2-307 [» ]
1DV6 X-ray 2.50 M/S 2-307 [» ]
1E14 X-ray 2.70 M 2-308 [» ]
1E6D X-ray 2.30 M 2-308 [» ]
1F6N X-ray 2.80 M 2-308 [» ]
1FNP X-ray 2.60 M 2-308 [» ]
1FNQ X-ray 2.60 M 2-308 [» ]
1JGW X-ray 2.80 M 2-308 [» ]
1JGX X-ray 3.01 M 2-308 [» ]
1JGY X-ray 2.70 M 2-308 [» ]
1JGZ X-ray 2.70 M 2-308 [» ]
1JH0 X-ray 3.50 M 2-308 [» ]
1K6L X-ray 3.10 M 2-308 [» ]
1K6N X-ray 3.10 M 2-308 [» ]
1KBY X-ray 2.50 M 2-308 [» ]
1L9B X-ray 2.40 M 2-308 [» ]
1L9J X-ray 3.25 M/S 2-308 [» ]
1M3X X-ray 2.55 M 2-308 [» ]
1MPS X-ray 2.55 M 2-308 [» ]
1OGV X-ray 2.35 M 2-308 [» ]
1PCR X-ray 2.65 M 2-308 [» ]
1PSS X-ray 3.00 M 7-302 [» ]
1PST X-ray 3.00 M 7-302 [» ]
1QOV X-ray 2.10 M 2-308 [» ]
1RG5 X-ray 2.50 M 2-308 [» ]
1RGN X-ray 2.80 M 2-308 [» ]
1RQK X-ray 2.70 M 2-308 [» ]
1RVJ X-ray 2.75 M 2-307 [» ]
1RY5 X-ray 2.10 M 2-308 [» ]
1RZH X-ray 1.80 M 2-308 [» ]
1RZZ X-ray 2.40 M/S 2-308 [» ]
1S00 X-ray 2.60 M/S 2-308 [» ]
1UMX X-ray 2.80 M 2-308 [» ]
1YF6 X-ray 2.25 M 2-308 [» ]
1YST X-ray 3.00 M 2-306 [» ]
1Z9J X-ray 4.50 B 2-308 [» ]
1Z9K X-ray 4.60 B 2-308 [» ]
2BNP X-ray 2.70 B 2-308 [» ]
2BNS X-ray 2.50 B 2-308 [» ]
2BOZ X-ray 2.40 M 2-308 [» ]
2GMR X-ray 2.50 M 2-308 [» ]
2GNU X-ray 2.20 M 3-302 [» ]
2HG3 X-ray 2.70 M 2-308 [» ]
2HG9 X-ray 2.45 M 2-308 [» ]
2HH1 X-ray 2.55 M 2-308 [» ]
2HHK X-ray 2.50 M 2-308 [» ]
2HIT X-ray 2.75 M 2-308 [» ]
2HJ6 X-ray 3.00 M 2-308 [» ]
2J8C X-ray 1.87 M 2-308 [» ]
2J8D X-ray 2.07 M 2-308 [» ]
2JIY X-ray 2.20 M 1-308 [» ]
2JJ0 X-ray 2.80 M 2-308 [» ]
2RCR X-ray 3.10 M 2-308 [» ]
2UWS X-ray 2.90 M 2-308 [» ]
2UWT X-ray 2.50 M 2-308 [» ]
2UWU X-ray 2.04 M 2-308 [» ]
2UWV X-ray 2.13 M 2-308 [» ]
2UWW X-ray 2.05 M 2-308 [» ]
2UX3 X-ray 2.50 M 2-308 [» ]
2UX4 X-ray 2.51 M 2-308 [» ]
2UX5 X-ray 2.21 M 2-308 [» ]
2UXJ X-ray 2.25 M 2-308 [» ]
2UXK X-ray 2.31 M 2-308 [» ]
2UXL X-ray 2.88 M 2-308 [» ]
2UXM X-ray 2.70 M 2-308 [» ]
3DSY X-ray 3.00 M 2-308 [» ]
3DTA X-ray 3.20 M 2-308 [» ]
3DTR X-ray 3.10 M 2-308 [» ]
3DTS X-ray 3.10 M 2-308 [» ]
3DU2 X-ray 3.10 M 2-308 [» ]
3DU3 X-ray 2.80 M 2-308 [» ]
3DUQ X-ray 2.70 M 2-308 [» ]
3I4D X-ray 2.01 M 2-308 [» ]
3V3Y X-ray 2.80 M 2-303 [» ]
3V3Z X-ray 2.90 M 2-303 [» ]
3ZUM X-ray 2.50 M 2-308 [» ]
3ZUW X-ray 2.31 M 2-308 [» ]
4H99 X-ray 2.97 M 2-303 [» ]
4H9L X-ray 2.77 M 2-303 [» ]
4HBH X-ray 2.93 M 2-303 [» ]
4HBJ X-ray 2.74 M 2-303 [» ]
4IN7 X-ray 2.85 M 2-303 [» ]
4JC9 X-ray 7.78 M 1-308 [» ]
4JCB X-ray 7.78 M 1-308 [» ]
4LWY X-ray 2.90 M 1-303 [» ]
4N7K X-ray 2.85 M 2-304 [» ]
4RCR X-ray 2.80 M 2-308 [» ]
4TQQ X-ray 2.50 M 2-303 [» ]
ProteinModelPortali P0C0Y9.
SMRi P0C0Y9. Positions 2-308.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60490N.

Protein family/group databases

TCDBi 3.E.2.1.1. the photosynthetic reaction center (prc) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P0C0Y9.

Family and domain databases

Gene3Di 1.20.85.10. 2 hits.
InterProi IPR000484. Photo_RC_L/M.
IPR005781. Photo_RC_M.
[Graphical view ]
Pfami PF00124. Photo_RC. 1 hit.
[Graphical view ]
PRINTSi PR00256. REACTNCENTRE.
SUPFAMi SSF81483. SSF81483. 1 hit.
TIGRFAMsi TIGR01115. pufM. 1 hit.
PROSITEi PS00244. REACTION_CENTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of the M subunit of the reaction center from Rhodopseudomonas sphaeroides."
    Williams J.C., Steiner L.A., Ogden R.C., Simon M.I., Feher G.
    Proc. Natl. Acad. Sci. U.S.A. 80:6505-6509(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Towards the understanding of the function of Rb sphaeroides Y wild type reaction center: gene cloning, protein and detergent structures in the three-dimensional crystals."
    Arnoux B., Ducruix A., Astier C., Picaud M., Roth M., Reiss-Husson F.
    Biochimie 72:525-530(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Y.
  3. "Primary structure of the L subunit of the reaction center from Rhodopseudomonas sphaeroides."
    Williams J.C., Steiner L.A., Feher G., Simon M.I.
    Proc. Natl. Acad. Sci. U.S.A. 81:7303-7307(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
  4. "Structure of the membrane-bound protein photosynthetic reaction center from Rhodobacter sphaeroides."
    Chang C.-H., El-Kabbani O., Tiede D., Norris J., Schiffer M.
    Biochemistry 30:5352-5360(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
  5. "Structure of the reaction center from Rhodobacter sphaeroides R-26: protein-cofactor (quinones and Fe2+) interactions."
    Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.
    Proc. Natl. Acad. Sci. U.S.A. 85:8487-8491(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    Strain: R-26.
  6. "Structure of the reaction center from Rhodobacter sphaeroides R-26: the protein subunits."
    Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.
    Proc. Natl. Acad. Sci. U.S.A. 84:6162-6166(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    Strain: R-26.
  7. "Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: monitoring the optical properties of the complex from bacterial cell to crystal."
    McAuley-Hecht K.E., Fyfe P.K., Ridge J.P., Prince S.M., Hunter C.N., Isaacs N.W., Cogdell R.J., Jones M.R.
    Biochemistry 37:4740-4750(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).

Entry informationi

Entry nameiRCEM_RHOSH
AccessioniPrimary (citable) accession number: P0C0Y9
Secondary accession number(s): P02953, Q9RFB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3