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Protein

Reaction center protein M chain

Gene

pufM

Organism
Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi183Magnesium (bacteriochlorophyll b axial ligand)1
Metal bindingi203Magnesium (bacteriochlorophyll b axial ligand)1
Metal bindingi220Iron1
Metal bindingi235Iron1
Binding sitei253Quinone A1
Metal bindingi267Iron1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Bacteriochlorophyll, Chlorophyll, Chromophore, Iron, Magnesium, Metal-binding

Protein family/group databases

TCDBi3.E.2.1.1. the photosynthetic reaction center (prc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Reaction center protein M chain
Alternative name(s):
Photosynthetic reaction center M subunit
Gene namesi
Name:pufM
OrganismiRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Taxonomic identifieri1063 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei54 – 80HelicalAdd BLAST27
Transmembranei111 – 140HelicalAdd BLAST30
Transmembranei143 – 168HelicalAdd BLAST26
Transmembranei198 – 226HelicalAdd BLAST29
Transmembranei260 – 286HelicalAdd BLAST27

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Reaction center

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000904172 – 308Reaction center protein M chainAdd BLAST307

Interactioni

Subunit structurei

Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).

Protein-protein interaction databases

DIPiDIP-60490N.
STRINGi349102.Rsph17025_2029.

Structurei

Secondary structure

1308
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 8Combined sources3
Beta strandi10 – 14Combined sources5
Beta strandi24 – 26Combined sources3
Helixi27 – 29Combined sources3
Helixi38 – 41Combined sources4
Beta strandi46 – 48Combined sources3
Helixi55 – 78Combined sources24
Turni79 – 81Combined sources3
Helixi83 – 88Combined sources6
Turni90 – 92Combined sources3
Helixi100 – 102Combined sources3
Beta strandi104 – 106Combined sources3
Helixi110 – 112Combined sources3
Helixi114 – 140Combined sources27
Helixi146 – 162Combined sources17
Helixi164 – 169Combined sources6
Helixi172 – 174Combined sources3
Helixi180 – 193Combined sources14
Helixi197 – 199Combined sources3
Helixi201 – 226Combined sources26
Helixi228 – 230Combined sources3
Turni231 – 233Combined sources3
Helixi235 – 240Combined sources6
Helixi244 – 257Combined sources14
Helixi265 – 286Combined sources22
Turni289 – 291Combined sources3
Beta strandi292 – 294Combined sources3
Helixi295 – 301Combined sources7
Beta strandi302 – 306Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AIGX-ray2.60M/O2-308[»]
1AIJX-ray2.20M/S2-308[»]
1DS8X-ray2.50M/S2-307[»]
1DV3X-ray2.50M/S2-307[»]
1DV6X-ray2.50M/S2-307[»]
1E14X-ray2.70M2-308[»]
1E6DX-ray2.30M2-308[»]
1F6NX-ray2.80M2-308[»]
1FNPX-ray2.60M2-308[»]
1FNQX-ray2.60M2-308[»]
1JGWX-ray2.80M2-308[»]
1JGXX-ray3.01M2-308[»]
1JGYX-ray2.70M2-308[»]
1JGZX-ray2.70M2-308[»]
1JH0X-ray3.50M2-308[»]
1K6LX-ray3.10M2-308[»]
1K6NX-ray3.10M2-308[»]
1KBYX-ray2.50M2-308[»]
1L9BX-ray2.40M2-308[»]
1L9JX-ray3.25M/S2-308[»]
1M3XX-ray2.55M2-308[»]
1MPSX-ray2.55M2-308[»]
1OGVX-ray2.35M2-308[»]
1PCRX-ray2.65M2-308[»]
1PSSX-ray3.00M7-302[»]
1PSTX-ray3.00M7-302[»]
1QOVX-ray2.10M2-308[»]
1RG5X-ray2.50M2-308[»]
1RGNX-ray2.80M2-308[»]
1RQKX-ray2.70M2-308[»]
1RVJX-ray2.75M2-307[»]
1RY5X-ray2.10M2-308[»]
1RZHX-ray1.80M2-308[»]
1RZZX-ray2.40M/S2-308[»]
1S00X-ray2.60M/S2-308[»]
1UMXX-ray2.80M2-308[»]
1YF6X-ray2.25M2-308[»]
1YSTX-ray3.00M2-306[»]
1Z9JX-ray4.50B2-308[»]
1Z9KX-ray4.60B2-308[»]
2BNPX-ray2.70B2-308[»]
2BNSX-ray2.50B2-308[»]
2BOZX-ray2.40M2-308[»]
2GMRX-ray2.50M2-308[»]
2GNUX-ray2.20M3-302[»]
2HG3X-ray2.70M2-308[»]
2HG9X-ray2.45M2-308[»]
2HH1X-ray2.55M2-308[»]
2HHKX-ray2.50M2-308[»]
2HITX-ray2.75M2-308[»]
2HJ6X-ray3.00M2-308[»]
2J8CX-ray1.87M2-308[»]
2J8DX-ray2.07M2-308[»]
2JIYX-ray2.20M1-308[»]
2JJ0X-ray2.80M2-308[»]
2RCRX-ray3.10M2-308[»]
2UWSX-ray2.90M2-308[»]
2UWTX-ray2.50M2-308[»]
2UWUX-ray2.04M2-308[»]
2UWVX-ray2.13M2-308[»]
2UWWX-ray2.05M2-308[»]
2UX3X-ray2.50M2-308[»]
2UX4X-ray2.51M2-308[»]
2UX5X-ray2.21M2-308[»]
2UXJX-ray2.25M2-308[»]
2UXKX-ray2.31M2-308[»]
2UXLX-ray2.88M2-308[»]
2UXMX-ray2.70M2-308[»]
3DSYX-ray3.00M2-308[»]
3DTAX-ray3.20M2-308[»]
3DTRX-ray3.10M2-308[»]
3DTSX-ray3.10M2-308[»]
3DU2X-ray3.10M2-308[»]
3DU3X-ray2.80M2-308[»]
3DUQX-ray2.70M2-308[»]
3I4DX-ray2.01M2-308[»]
3V3YX-ray2.80M2-303[»]
3V3ZX-ray2.90M2-303[»]
3ZUMX-ray2.50M2-308[»]
3ZUWX-ray2.31M2-308[»]
4H99X-ray2.97M2-303[»]
4H9LX-ray2.77M2-303[»]
4HBHX-ray2.93M2-303[»]
4HBJX-ray2.74M2-303[»]
4IN7X-ray2.85M2-303[»]
4LWYX-ray2.90M1-303[»]
4N7KX-ray2.85M2-304[»]
4RCRX-ray2.80M2-308[»]
4TQQX-ray2.50M2-303[»]
4V9GX-ray7.78AM/BM1-308[»]
ProteinModelPortaliP0C0Y9.
SMRiP0C0Y9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0Y9.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107R94. Bacteria.
ENOG410ZVGN. LUCA.

Family and domain databases

CDDicd09291. Photo-RC_M. 1 hit.
Gene3Di1.20.85.10. 2 hits.
InterProiIPR000484. Photo_RC_L/M.
IPR005781. Photo_RC_M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01115. pufM. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C0Y9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEYQNIFSQ VQVRGPADLG MTEDVNLANR SGVGPFSTLL GWFGNAQLGP
60 70 80 90 100
IYLGSLGVLS LFSGLMWFFT IGIWFWYQAG WNPAVFLRDL FFFSLEPPAP
110 120 130 140 150
EYGLSFAAPL KEGGLWLIAS FFMFVAVWSW WGRTYLRAQA LGMGKHTAWA
160 170 180 190 200
FLSAIWLWMV LGFIRPILMG SWSEAVPYGI FSHLDWTNNF SLVHGNLFYN
210 220 230 240 250
PFHGLSIAFL YGSALLFAMH GATILAVSRF GGERELEQIA DRGTAAERAA
260 270 280 290 300
LFWRWTMGFN ATMEGIHRWA IWMAVLVTLT GGIGILLSGT VVDNWYVWGQ

NHGMAPLN
Length:308
Mass (Da):34,509
Last modified:January 23, 2007 - v2
Checksum:i30B3737DF8658250
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti141L → M in CAA45001 (PubMed:2126457).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00827 Genomic DNA. Translation: AAA26179.1.
X63405 Genomic DNA. Translation: CAA45001.1.
X63404 Genomic DNA. Translation: CAA45000.1.
M10206 Genomic DNA. Translation: AAA26178.1.
PIRiA03456. WNRFMS.
S24213.
RefSeqiWP_002720420.1. NZ_CP015210.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00827 Genomic DNA. Translation: AAA26179.1.
X63405 Genomic DNA. Translation: CAA45001.1.
X63404 Genomic DNA. Translation: CAA45000.1.
M10206 Genomic DNA. Translation: AAA26178.1.
PIRiA03456. WNRFMS.
S24213.
RefSeqiWP_002720420.1. NZ_CP015210.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AIGX-ray2.60M/O2-308[»]
1AIJX-ray2.20M/S2-308[»]
1DS8X-ray2.50M/S2-307[»]
1DV3X-ray2.50M/S2-307[»]
1DV6X-ray2.50M/S2-307[»]
1E14X-ray2.70M2-308[»]
1E6DX-ray2.30M2-308[»]
1F6NX-ray2.80M2-308[»]
1FNPX-ray2.60M2-308[»]
1FNQX-ray2.60M2-308[»]
1JGWX-ray2.80M2-308[»]
1JGXX-ray3.01M2-308[»]
1JGYX-ray2.70M2-308[»]
1JGZX-ray2.70M2-308[»]
1JH0X-ray3.50M2-308[»]
1K6LX-ray3.10M2-308[»]
1K6NX-ray3.10M2-308[»]
1KBYX-ray2.50M2-308[»]
1L9BX-ray2.40M2-308[»]
1L9JX-ray3.25M/S2-308[»]
1M3XX-ray2.55M2-308[»]
1MPSX-ray2.55M2-308[»]
1OGVX-ray2.35M2-308[»]
1PCRX-ray2.65M2-308[»]
1PSSX-ray3.00M7-302[»]
1PSTX-ray3.00M7-302[»]
1QOVX-ray2.10M2-308[»]
1RG5X-ray2.50M2-308[»]
1RGNX-ray2.80M2-308[»]
1RQKX-ray2.70M2-308[»]
1RVJX-ray2.75M2-307[»]
1RY5X-ray2.10M2-308[»]
1RZHX-ray1.80M2-308[»]
1RZZX-ray2.40M/S2-308[»]
1S00X-ray2.60M/S2-308[»]
1UMXX-ray2.80M2-308[»]
1YF6X-ray2.25M2-308[»]
1YSTX-ray3.00M2-306[»]
1Z9JX-ray4.50B2-308[»]
1Z9KX-ray4.60B2-308[»]
2BNPX-ray2.70B2-308[»]
2BNSX-ray2.50B2-308[»]
2BOZX-ray2.40M2-308[»]
2GMRX-ray2.50M2-308[»]
2GNUX-ray2.20M3-302[»]
2HG3X-ray2.70M2-308[»]
2HG9X-ray2.45M2-308[»]
2HH1X-ray2.55M2-308[»]
2HHKX-ray2.50M2-308[»]
2HITX-ray2.75M2-308[»]
2HJ6X-ray3.00M2-308[»]
2J8CX-ray1.87M2-308[»]
2J8DX-ray2.07M2-308[»]
2JIYX-ray2.20M1-308[»]
2JJ0X-ray2.80M2-308[»]
2RCRX-ray3.10M2-308[»]
2UWSX-ray2.90M2-308[»]
2UWTX-ray2.50M2-308[»]
2UWUX-ray2.04M2-308[»]
2UWVX-ray2.13M2-308[»]
2UWWX-ray2.05M2-308[»]
2UX3X-ray2.50M2-308[»]
2UX4X-ray2.51M2-308[»]
2UX5X-ray2.21M2-308[»]
2UXJX-ray2.25M2-308[»]
2UXKX-ray2.31M2-308[»]
2UXLX-ray2.88M2-308[»]
2UXMX-ray2.70M2-308[»]
3DSYX-ray3.00M2-308[»]
3DTAX-ray3.20M2-308[»]
3DTRX-ray3.10M2-308[»]
3DTSX-ray3.10M2-308[»]
3DU2X-ray3.10M2-308[»]
3DU3X-ray2.80M2-308[»]
3DUQX-ray2.70M2-308[»]
3I4DX-ray2.01M2-308[»]
3V3YX-ray2.80M2-303[»]
3V3ZX-ray2.90M2-303[»]
3ZUMX-ray2.50M2-308[»]
3ZUWX-ray2.31M2-308[»]
4H99X-ray2.97M2-303[»]
4H9LX-ray2.77M2-303[»]
4HBHX-ray2.93M2-303[»]
4HBJX-ray2.74M2-303[»]
4IN7X-ray2.85M2-303[»]
4LWYX-ray2.90M1-303[»]
4N7KX-ray2.85M2-304[»]
4RCRX-ray2.80M2-308[»]
4TQQX-ray2.50M2-303[»]
4V9GX-ray7.78AM/BM1-308[»]
ProteinModelPortaliP0C0Y9.
SMRiP0C0Y9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60490N.
STRINGi349102.Rsph17025_2029.

Protein family/group databases

TCDBi3.E.2.1.1. the photosynthetic reaction center (prc) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4107R94. Bacteria.
ENOG410ZVGN. LUCA.

Miscellaneous databases

EvolutionaryTraceiP0C0Y9.

Family and domain databases

CDDicd09291. Photo-RC_M. 1 hit.
Gene3Di1.20.85.10. 2 hits.
InterProiIPR000484. Photo_RC_L/M.
IPR005781. Photo_RC_M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01115. pufM. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRCEM_RHOSH
AccessioniPrimary (citable) accession number: P0C0Y9
Secondary accession number(s): P02953, Q9RFB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.