P0C0Y8 (RCEL_RHOSH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Reaction center protein L chain

Alternative name(s):
Photosynthetic reaction center L subunit

Gene names

Name:

pufL

Organism

Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)

Taxonomic identifier

1063 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
Subunit structure	Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).
Subcellular location	Cellular chromatophore membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the reaction center PufL/M/PsbA/D family.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Membrane Reaction center
Domain	Transmembrane Transmembrane helix
Ligand	Bacteriochlorophyll Chlorophyll Chromophore Iron Magnesium Metal-binding
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	photosynthetic electron transport in photosystem II Inferred from electronic annotation. Source: InterPro protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane light-harvesting complex Inferred from electronic annotation. Source: InterPro plasma membrane-derived chromatophore membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	bacteriochlorophyll binding Inferred from electronic annotation. Source: UniProtKB-KW electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 282

281

Reaction center protein L chain

PRO_0000090407

Regions

Topological domain

2 – 32

31

Cytoplasmic Ref.7

Transmembrane

33 – 56

24

Helical

Topological domain

57 – 83

27

Periplasmic Ref.7

Transmembrane

84 – 112

29

Helical

Topological domain

113 – 116

4

Cytoplasmic Ref.7

Transmembrane

117 – 139

23

Helical

Topological domain

140 – 171

32

Periplasmic Ref.7

Transmembrane

172 – 199

28

Helical

Topological domain

200 – 225

26

Cytoplasmic Ref.7

Transmembrane

226 – 251

26

Helical

Topological domain

252 – 282

31

Periplasmic Ref.7

Sites

Metal binding

154

1

Magnesium (bacteriochlorophyll b axial ligand)

Metal binding

174

1

Magnesium (bacteriochlorophyll b axial ligand)

Metal binding

191

1

Iron

Metal binding

231

1

Iron

Binding site

217

1

Quinone B

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

282

Helix Strand Turn

Details...

Turn

5 – 7

3

Helix

8 – 10

3

Beta strand

17 – 19

3

Turn

20 – 23

4

Beta strand

25 – 27

3

Beta strand

30 – 32

3

Helix

33 – 57

25

Turn

62 – 64

3

Helix

72 – 74

3

Helix

81 – 83

3

Helix

85 – 112

28

Helix

117 – 133

17

Helix

135 – 140

6

Helix

143 – 145

3

Helix

153 – 163

11

Helix

168 – 170

3

Helix

172 – 199

28

Beta strand

202 – 205

4

Helix

210 – 221

12

Helix

227 – 250

24

Turn

253 – 255

3

Helix

260 – 263

4

Helix

265 – 268

4

Turn

271 – 275

5

Beta strand

278 – 280

3

Sequences

	Sequence		Length	Mass (Da)
	P0C0Y8 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 044F4D3AF085B136	FASTA	282	31,457
	10 20 30 40 50 60 MALLSFERKY RVPGGTLVGG NLFDFWVGPF YVGFFGVATF FFAALGIILI AWSAVLQGTW 70 80 90 100 110 120 NPQLISVYPP ALEYGLGGAP LAKGGLWQII TICATGAFVS WALREVEICR KLGIGYHIPF 130 140 150 160 170 180 AFAFAILAYL TLVLFRPVMM GAWGYAFPYG IWTHLDWVSN TGYTYGNFHY NPAHMIAISF 190 200 210 220 230 240 FFTNALALAL HGALVLSAAN PEKGKEMRTP DHEDTFFRDL VGYSIGTLGI HRLGLLLSLS 250 260 270 280 AVFFSALCMI ITGTIWFDQW VDWWQWWVKL PWWANIPGGI NG

References

[1]	"Primary structure of the L subunit of the reaction center from Rhodopseudomonas sphaeroides." Williams J.C., Steiner L.A., Feher G., Simon M.I. Proc. Natl. Acad. Sci. U.S.A. 81:7303-7307(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Towards the understanding of the function of Rb sphaeroides Y wild type reaction center: gene cloning, protein and detergent structures in the three-dimensional crystals." Arnoux B., Ducruix A., Astier C., Picaud M., Roth M., Reiss-Husson F. Biochimie 72:525-530(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Y.
[3]	"Structure of the membrane-bound protein photosynthetic reaction center from Rhodobacter sphaeroides." Chang C.-H., El-Kabbani O., Tiede D., Norris J., Schiffer M. Biochemistry 30:5352-5360(1991) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
[4]	"Structure of the reaction center from Rhodobacter sphaeroides R-26: protein-cofactor (quinones and Fe2+) interactions." Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C. Proc. Natl. Acad. Sci. U.S.A. 85:8487-8491(1988) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS). Strain: R-26.
[5]	"Structure of the reaction center from Rhodobacter sphaeroides R-26: the protein subunits." Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C. Proc. Natl. Acad. Sci. U.S.A. 84:6162-6166(1987) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS). Strain: R-26.
[6]	"Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: monitoring the optical properties of the complex from bacterial cell to crystal." McAuley-Hecht K.E., Fyfe P.K., Ridge J.P., Prince S.M., Hunter C.N., Isaacs N.W., Cogdell R.J., Jones M.R. Biochemistry 37:4740-4750(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).
[7]	"The use of gene fusions to examine the membrane topology of the L-subunit of the photosynthetic reaction center and of the cytochrome b subunit of the bc1 complex from Rhodobacter sphaeroides." Yun C.H., Van Doren S.R., Crofts A.R., Gennis R.B. J. Biol. Chem. 266:10967-10973(1991) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M10206 Genomic DNA. Translation: AAA26177.1.
X63404 Genomic DNA. Translation: CAA44999.1.

PIR

WNRFLS. S24212.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AIG	X-ray	2.60	L/N	2-282	[»]
1AIJ	X-ray	2.20	L/R	2-282	[»]
1DS8	X-ray	2.50	L/R	2-282	[»]
1DV3	X-ray	2.50	L/R	2-282	[»]
1DV6	X-ray	2.50	L/R	2-282	[»]
1E14	X-ray	2.70	L	2-282	[»]
1E6D	X-ray	2.30	L	2-282	[»]
1F6N	X-ray	2.80	L	2-282	[»]
1FNP	X-ray	2.60	L	2-282	[»]
1FNQ	X-ray	2.60	L	2-282	[»]
1JGW	X-ray	2.80	L	2-282	[»]
1JGX	X-ray	3.01	L	2-282	[»]
1JGY	X-ray	2.70	L	2-282	[»]
1JGZ	X-ray	2.70	L	2-282	[»]
1JH0	X-ray	3.50	L	2-282	[»]
1K6L	X-ray	3.10	L	2-282	[»]
1K6N	X-ray	3.10	L	2-282	[»]
1KBY	X-ray	2.50	L	2-282	[»]
1L9B	X-ray	2.40	L	2-282	[»]
1L9J	X-ray	3.25	L/R	2-282	[»]
1M3X	X-ray	2.55	L	2-282	[»]
1MPS	X-ray	2.55	L	2-282	[»]
1OGV	X-ray	2.35	L	2-282	[»]
1PCR	X-ray	2.65	L	2-282	[»]
1PSS	X-ray	3.00	L	6-271	[»]
1PST	X-ray	3.00	L	6-271	[»]
1QOV	X-ray	2.10	L	2-282	[»]
1RG5	X-ray	2.50	L	2-282	[»]
1RGN	X-ray	2.80	L	2-282	[»]
1RQK	X-ray	2.70	L	2-282	[»]
1RVJ	X-ray	2.75	L	2-282	[»]
1RY5	X-ray	2.10	L	2-282	[»]
1RZH	X-ray	1.80	L	2-282	[»]
1RZZ	X-ray	2.40	L/R	2-282	[»]
1S00	X-ray	2.60	L/R	2-282	[»]
1UMX	X-ray	2.80	L	2-282	[»]
1YF6	X-ray	2.25	L	2-282	[»]
1YST	X-ray	3.00	L	2-274	[»]
1Z9J	X-ray	4.50	A	2-282	[»]
1Z9K	X-ray	4.60	A	2-282	[»]
2BNP	X-ray	2.70	A	2-282	[»]
2BNS	X-ray	2.50	A	2-282	[»]
2BOZ	X-ray	2.40	L	2-282	[»]
2GMR	X-ray	2.50	L	2-282	[»]
2GNU	X-ray	2.20	L	2-281	[»]
2HG3	X-ray	2.70	L	2-281	[»]
2HG9	X-ray	2.45	L	2-281	[»]
2HH1	X-ray	2.55	L	2-281	[»]
2HHK	X-ray	2.50	L	2-281	[»]
2HIT	X-ray	2.75	L	2-281	[»]
2HJ6	X-ray	3.00	L	2-281	[»]
2J8C	X-ray	1.87	L	2-281	[»]
2J8D	X-ray	2.07	L	2-281	[»]
2JIY	X-ray	2.20	L	2-282	[»]
2JJ0	X-ray	2.80	L	2-281	[»]
2RCR	X-ray	3.10	L	2-282	[»]
2UWS	X-ray	2.90	L	2-281	[»]
2UWT	X-ray	2.50	L	2-281	[»]
2UWU	X-ray	2.04	L	2-281	[»]
2UWV	X-ray	2.13	L	2-281	[»]
2UWW	X-ray	2.05	L	2-281	[»]
2UX3	X-ray	2.50	L	2-281	[»]
2UX4	X-ray	2.51	L	2-281	[»]
2UX5	X-ray	2.21	L	2-281	[»]
2UXJ	X-ray	2.25	L	2-281	[»]
2UXK	X-ray	2.31	L	2-281	[»]
2UXL	X-ray	2.88	L	2-281	[»]
2UXM	X-ray	2.70	L	2-281	[»]
3DSY	X-ray	3.00	L	2-282	[»]
3DTA	X-ray	3.20	L	2-282	[»]
3DTR	X-ray	3.10	L	2-282	[»]
3DTS	X-ray	3.10	L	2-282	[»]
3DU2	X-ray	3.10	L	2-282	[»]
3DU3	X-ray	2.80	L	2-282	[»]
3DUQ	X-ray	2.70	L	2-282	[»]
3I4D	X-ray	2.01	L	2-282	[»]
3V3Y	X-ray	2.80	L	2-282	[»]
3V3Z	X-ray	2.90	L	2-282	[»]
3ZUM	X-ray	2.50	L	2-282	[»]
3ZUW	X-ray	2.31	L	2-282	[»]
4H99	X-ray	2.97	L	2-282	[»]
4H9L	X-ray	2.77	L	2-282	[»]
4HBH	X-ray	2.93	L	2-282	[»]
4HBJ	X-ray	2.74	L	2-282	[»]
4RCR	X-ray	2.80	L	2-282	[»]

ProteinModelPortal

P0C0Y8.

SMR

P0C0Y8. Positions 2-282.

ModBase

Search...

Protein family/group databases

TCDB

3.E.2.1.1. photosynthetic reaction center (PRC) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.20.85.10. 2 hits.

InterPro

IPR005871. Photo_RC_L.
IPR000484. Photo_RC_L/M.
[Graphical view]

Pfam

PF00124. Photo_RC. 1 hit.
[Graphical view]

PRINTS

PR00256. REACTNCENTRE.

SUPFAM

SSF81483. Photo_RC_L/M. 1 hit.

TIGRFAMs

TIGR01157. pufL. 1 hit.

PROSITE

PS00244. REACTION_CENTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0C0Y8.

Entry information

Entry name

RCEL_RHOSH

Accession

Primary (citable) accession number: P0C0Y8
Secondary accession number(s): P02954, Q9RFB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program