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P0C0Y8

- RCEL_RHOSH

UniProt

P0C0Y8 - RCEL_RHOSH

Protein

Reaction center protein L chain

Gene

pufL

Organism
Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi154 – 1541Magnesium (bacteriochlorophyll b axial ligand)
    Metal bindingi174 – 1741Magnesium (bacteriochlorophyll b axial ligand)
    Metal bindingi191 – 1911Iron
    Binding sitei217 – 2171Quinone B
    Metal bindingi231 – 2311Iron

    GO - Molecular functioni

    1. bacteriochlorophyll binding Source: UniProtKB-KW
    2. electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. photosynthetic electron transport in photosystem II Source: InterPro
    2. protein-chromophore linkage Source: UniProtKB-KW

    Keywords - Biological processi

    Electron transport, Photosynthesis, Transport

    Keywords - Ligandi

    Bacteriochlorophyll, Chlorophyll, Chromophore, Iron, Magnesium, Metal-binding

    Protein family/group databases

    TCDBi3.E.2.1.1. the photosynthetic reaction center (prc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Reaction center protein L chain
    Alternative name(s):
    Photosynthetic reaction center L subunit
    Gene namesi
    Name:pufL
    OrganismiRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
    Taxonomic identifieri1063 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane-derived chromatophore membrane Source: UniProtKB-SubCell
    3. plasma membrane light-harvesting complex Source: InterPro

    Keywords - Cellular componenti

    Membrane, Reaction center

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 282281Reaction center protein L chainPRO_0000090407Add
    BLAST

    Interactioni

    Subunit structurei

    Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).

    Protein-protein interaction databases

    DIPiDIP-60489N.

    Structurei

    Secondary structure

    1
    282
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni5 – 73
    Helixi8 – 103
    Beta strandi17 – 193
    Turni20 – 234
    Beta strandi25 – 273
    Beta strandi30 – 323
    Helixi33 – 5725
    Turni62 – 643
    Helixi72 – 743
    Helixi81 – 833
    Helixi85 – 11228
    Helixi117 – 13317
    Helixi135 – 1406
    Helixi143 – 1453
    Helixi153 – 16311
    Helixi168 – 1703
    Helixi172 – 19928
    Beta strandi202 – 2054
    Helixi210 – 22112
    Helixi227 – 25024
    Turni253 – 2553
    Helixi260 – 2634
    Helixi265 – 2684
    Turni271 – 2755
    Beta strandi278 – 2803

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AIGX-ray2.60L/N2-282[»]
    1AIJX-ray2.20L/R2-282[»]
    1DS8X-ray2.50L/R2-282[»]
    1DV3X-ray2.50L/R2-282[»]
    1DV6X-ray2.50L/R2-282[»]
    1E14X-ray2.70L2-282[»]
    1E6DX-ray2.30L2-282[»]
    1F6NX-ray2.80L2-282[»]
    1FNPX-ray2.60L2-282[»]
    1FNQX-ray2.60L2-282[»]
    1JGWX-ray2.80L2-282[»]
    1JGXX-ray3.01L2-282[»]
    1JGYX-ray2.70L2-282[»]
    1JGZX-ray2.70L2-282[»]
    1JH0X-ray3.50L2-282[»]
    1K6LX-ray3.10L2-282[»]
    1K6NX-ray3.10L2-282[»]
    1KBYX-ray2.50L2-282[»]
    1L9BX-ray2.40L2-282[»]
    1L9JX-ray3.25L/R2-282[»]
    1M3XX-ray2.55L2-282[»]
    1MPSX-ray2.55L2-282[»]
    1OGVX-ray2.35L2-282[»]
    1PCRX-ray2.65L2-282[»]
    1PSSX-ray3.00L6-271[»]
    1PSTX-ray3.00L6-271[»]
    1QOVX-ray2.10L2-282[»]
    1RG5X-ray2.50L2-282[»]
    1RGNX-ray2.80L2-282[»]
    1RQKX-ray2.70L2-282[»]
    1RVJX-ray2.75L2-282[»]
    1RY5X-ray2.10L2-282[»]
    1RZHX-ray1.80L2-282[»]
    1RZZX-ray2.40L/R2-282[»]
    1S00X-ray2.60L/R2-282[»]
    1UMXX-ray2.80L2-282[»]
    1YF6X-ray2.25L2-282[»]
    1YSTX-ray3.00L2-274[»]
    1Z9JX-ray4.50A2-282[»]
    1Z9KX-ray4.60A2-282[»]
    2BNPX-ray2.70A2-282[»]
    2BNSX-ray2.50A2-282[»]
    2BOZX-ray2.40L2-282[»]
    2GMRX-ray2.50L2-282[»]
    2GNUX-ray2.20L2-282[»]
    2HG3X-ray2.70L2-282[»]
    2HG9X-ray2.45L2-282[»]
    2HH1X-ray2.55L2-282[»]
    2HHKX-ray2.50L2-282[»]
    2HITX-ray2.75L2-282[»]
    2HJ6X-ray3.00L2-282[»]
    2J8CX-ray1.87L2-282[»]
    2J8DX-ray2.07L2-282[»]
    2JIYX-ray2.20L2-282[»]
    2JJ0X-ray2.80L2-282[»]
    2RCRX-ray3.10L2-282[»]
    2UWSX-ray2.90L2-282[»]
    2UWTX-ray2.50L2-282[»]
    2UWUX-ray2.04L2-282[»]
    2UWVX-ray2.13L2-282[»]
    2UWWX-ray2.05L2-282[»]
    2UX3X-ray2.50L2-282[»]
    2UX4X-ray2.51L2-282[»]
    2UX5X-ray2.21L2-282[»]
    2UXJX-ray2.25L2-282[»]
    2UXKX-ray2.31L2-282[»]
    2UXLX-ray2.88L2-282[»]
    2UXMX-ray2.70L2-282[»]
    3DSYX-ray3.00L2-282[»]
    3DTAX-ray3.20L2-282[»]
    3DTRX-ray3.10L2-282[»]
    3DTSX-ray3.10L2-282[»]
    3DU2X-ray3.10L2-282[»]
    3DU3X-ray2.80L2-282[»]
    3DUQX-ray2.70L2-282[»]
    3I4DX-ray2.01L2-282[»]
    3V3YX-ray2.80L2-282[»]
    3V3ZX-ray2.90L2-282[»]
    3ZUMX-ray2.50L2-282[»]
    3ZUWX-ray2.31L2-282[»]
    4H99X-ray2.97L2-282[»]
    4H9LX-ray2.77L2-282[»]
    4HBHX-ray2.93L2-282[»]
    4HBJX-ray2.74L2-282[»]
    4IN7X-ray2.85L2-282[»]
    4JC9X-ray7.78L1-282[»]
    4JCBX-ray7.78L1-282[»]
    4N7KX-ray2.85L2-282[»]
    4RCRX-ray2.80L2-282[»]
    ProteinModelPortaliP0C0Y8.
    SMRiP0C0Y8. Positions 2-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0C0Y8.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 3231Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini57 – 8327Periplasmic1 PublicationAdd
    BLAST
    Topological domaini113 – 1164Cytoplasmic1 Publication
    Topological domaini140 – 17132Periplasmic1 PublicationAdd
    BLAST
    Topological domaini200 – 22526Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini252 – 28231Periplasmic1 PublicationAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei33 – 5624HelicalAdd
    BLAST
    Transmembranei84 – 11229HelicalAdd
    BLAST
    Transmembranei117 – 13923HelicalAdd
    BLAST
    Transmembranei172 – 19928HelicalAdd
    BLAST
    Transmembranei226 – 25126HelicalAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.20.85.10. 2 hits.
    InterProiIPR005871. Photo_RC_L.
    IPR000484. Photo_RC_L/M.
    [Graphical view]
    PfamiPF00124. Photo_RC. 1 hit.
    [Graphical view]
    PRINTSiPR00256. REACTNCENTRE.
    SUPFAMiSSF81483. SSF81483. 1 hit.
    TIGRFAMsiTIGR01157. pufL. 1 hit.
    PROSITEiPS00244. REACTION_CENTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0C0Y8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALLSFERKY RVPGGTLVGG NLFDFWVGPF YVGFFGVATF FFAALGIILI    50
    AWSAVLQGTW NPQLISVYPP ALEYGLGGAP LAKGGLWQII TICATGAFVS 100
    WALREVEICR KLGIGYHIPF AFAFAILAYL TLVLFRPVMM GAWGYAFPYG 150
    IWTHLDWVSN TGYTYGNFHY NPAHMIAISF FFTNALALAL HGALVLSAAN 200
    PEKGKEMRTP DHEDTFFRDL VGYSIGTLGI HRLGLLLSLS AVFFSALCMI 250
    ITGTIWFDQW VDWWQWWVKL PWWANIPGGI NG 282
    Length:282
    Mass (Da):31,457
    Last modified:January 23, 2007 - v2
    Checksum:i044F4D3AF085B136
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10206 Genomic DNA. Translation: AAA26177.1.
    X63404 Genomic DNA. Translation: CAA44999.1.
    PIRiS24212. WNRFLS.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10206 Genomic DNA. Translation: AAA26177.1 .
    X63404 Genomic DNA. Translation: CAA44999.1 .
    PIRi S24212. WNRFLS.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AIG X-ray 2.60 L/N 2-282 [» ]
    1AIJ X-ray 2.20 L/R 2-282 [» ]
    1DS8 X-ray 2.50 L/R 2-282 [» ]
    1DV3 X-ray 2.50 L/R 2-282 [» ]
    1DV6 X-ray 2.50 L/R 2-282 [» ]
    1E14 X-ray 2.70 L 2-282 [» ]
    1E6D X-ray 2.30 L 2-282 [» ]
    1F6N X-ray 2.80 L 2-282 [» ]
    1FNP X-ray 2.60 L 2-282 [» ]
    1FNQ X-ray 2.60 L 2-282 [» ]
    1JGW X-ray 2.80 L 2-282 [» ]
    1JGX X-ray 3.01 L 2-282 [» ]
    1JGY X-ray 2.70 L 2-282 [» ]
    1JGZ X-ray 2.70 L 2-282 [» ]
    1JH0 X-ray 3.50 L 2-282 [» ]
    1K6L X-ray 3.10 L 2-282 [» ]
    1K6N X-ray 3.10 L 2-282 [» ]
    1KBY X-ray 2.50 L 2-282 [» ]
    1L9B X-ray 2.40 L 2-282 [» ]
    1L9J X-ray 3.25 L/R 2-282 [» ]
    1M3X X-ray 2.55 L 2-282 [» ]
    1MPS X-ray 2.55 L 2-282 [» ]
    1OGV X-ray 2.35 L 2-282 [» ]
    1PCR X-ray 2.65 L 2-282 [» ]
    1PSS X-ray 3.00 L 6-271 [» ]
    1PST X-ray 3.00 L 6-271 [» ]
    1QOV X-ray 2.10 L 2-282 [» ]
    1RG5 X-ray 2.50 L 2-282 [» ]
    1RGN X-ray 2.80 L 2-282 [» ]
    1RQK X-ray 2.70 L 2-282 [» ]
    1RVJ X-ray 2.75 L 2-282 [» ]
    1RY5 X-ray 2.10 L 2-282 [» ]
    1RZH X-ray 1.80 L 2-282 [» ]
    1RZZ X-ray 2.40 L/R 2-282 [» ]
    1S00 X-ray 2.60 L/R 2-282 [» ]
    1UMX X-ray 2.80 L 2-282 [» ]
    1YF6 X-ray 2.25 L 2-282 [» ]
    1YST X-ray 3.00 L 2-274 [» ]
    1Z9J X-ray 4.50 A 2-282 [» ]
    1Z9K X-ray 4.60 A 2-282 [» ]
    2BNP X-ray 2.70 A 2-282 [» ]
    2BNS X-ray 2.50 A 2-282 [» ]
    2BOZ X-ray 2.40 L 2-282 [» ]
    2GMR X-ray 2.50 L 2-282 [» ]
    2GNU X-ray 2.20 L 2-282 [» ]
    2HG3 X-ray 2.70 L 2-282 [» ]
    2HG9 X-ray 2.45 L 2-282 [» ]
    2HH1 X-ray 2.55 L 2-282 [» ]
    2HHK X-ray 2.50 L 2-282 [» ]
    2HIT X-ray 2.75 L 2-282 [» ]
    2HJ6 X-ray 3.00 L 2-282 [» ]
    2J8C X-ray 1.87 L 2-282 [» ]
    2J8D X-ray 2.07 L 2-282 [» ]
    2JIY X-ray 2.20 L 2-282 [» ]
    2JJ0 X-ray 2.80 L 2-282 [» ]
    2RCR X-ray 3.10 L 2-282 [» ]
    2UWS X-ray 2.90 L 2-282 [» ]
    2UWT X-ray 2.50 L 2-282 [» ]
    2UWU X-ray 2.04 L 2-282 [» ]
    2UWV X-ray 2.13 L 2-282 [» ]
    2UWW X-ray 2.05 L 2-282 [» ]
    2UX3 X-ray 2.50 L 2-282 [» ]
    2UX4 X-ray 2.51 L 2-282 [» ]
    2UX5 X-ray 2.21 L 2-282 [» ]
    2UXJ X-ray 2.25 L 2-282 [» ]
    2UXK X-ray 2.31 L 2-282 [» ]
    2UXL X-ray 2.88 L 2-282 [» ]
    2UXM X-ray 2.70 L 2-282 [» ]
    3DSY X-ray 3.00 L 2-282 [» ]
    3DTA X-ray 3.20 L 2-282 [» ]
    3DTR X-ray 3.10 L 2-282 [» ]
    3DTS X-ray 3.10 L 2-282 [» ]
    3DU2 X-ray 3.10 L 2-282 [» ]
    3DU3 X-ray 2.80 L 2-282 [» ]
    3DUQ X-ray 2.70 L 2-282 [» ]
    3I4D X-ray 2.01 L 2-282 [» ]
    3V3Y X-ray 2.80 L 2-282 [» ]
    3V3Z X-ray 2.90 L 2-282 [» ]
    3ZUM X-ray 2.50 L 2-282 [» ]
    3ZUW X-ray 2.31 L 2-282 [» ]
    4H99 X-ray 2.97 L 2-282 [» ]
    4H9L X-ray 2.77 L 2-282 [» ]
    4HBH X-ray 2.93 L 2-282 [» ]
    4HBJ X-ray 2.74 L 2-282 [» ]
    4IN7 X-ray 2.85 L 2-282 [» ]
    4JC9 X-ray 7.78 L 1-282 [» ]
    4JCB X-ray 7.78 L 1-282 [» ]
    4N7K X-ray 2.85 L 2-282 [» ]
    4RCR X-ray 2.80 L 2-282 [» ]
    ProteinModelPortali P0C0Y8.
    SMRi P0C0Y8. Positions 2-282.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60489N.

    Protein family/group databases

    TCDBi 3.E.2.1.1. the photosynthetic reaction center (prc) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P0C0Y8.

    Family and domain databases

    Gene3Di 1.20.85.10. 2 hits.
    InterProi IPR005871. Photo_RC_L.
    IPR000484. Photo_RC_L/M.
    [Graphical view ]
    Pfami PF00124. Photo_RC. 1 hit.
    [Graphical view ]
    PRINTSi PR00256. REACTNCENTRE.
    SUPFAMi SSF81483. SSF81483. 1 hit.
    TIGRFAMsi TIGR01157. pufL. 1 hit.
    PROSITEi PS00244. REACTION_CENTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the L subunit of the reaction center from Rhodopseudomonas sphaeroides."
      Williams J.C., Steiner L.A., Feher G., Simon M.I.
      Proc. Natl. Acad. Sci. U.S.A. 81:7303-7307(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Towards the understanding of the function of Rb sphaeroides Y wild type reaction center: gene cloning, protein and detergent structures in the three-dimensional crystals."
      Arnoux B., Ducruix A., Astier C., Picaud M., Roth M., Reiss-Husson F.
      Biochimie 72:525-530(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Y.
    3. "Structure of the membrane-bound protein photosynthetic reaction center from Rhodobacter sphaeroides."
      Chang C.-H., El-Kabbani O., Tiede D., Norris J., Schiffer M.
      Biochemistry 30:5352-5360(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
    4. "Structure of the reaction center from Rhodobacter sphaeroides R-26: protein-cofactor (quinones and Fe2+) interactions."
      Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.
      Proc. Natl. Acad. Sci. U.S.A. 85:8487-8491(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
      Strain: R-26.
    5. "Structure of the reaction center from Rhodobacter sphaeroides R-26: the protein subunits."
      Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.
      Proc. Natl. Acad. Sci. U.S.A. 84:6162-6166(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
      Strain: R-26.
    6. "Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: monitoring the optical properties of the complex from bacterial cell to crystal."
      McAuley-Hecht K.E., Fyfe P.K., Ridge J.P., Prince S.M., Hunter C.N., Isaacs N.W., Cogdell R.J., Jones M.R.
      Biochemistry 37:4740-4750(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).
    7. "The use of gene fusions to examine the membrane topology of the L-subunit of the photosynthetic reaction center and of the cytochrome b subunit of the bc1 complex from Rhodobacter sphaeroides."
      Yun C.H., Van Doren S.R., Crofts A.R., Gennis R.B.
      J. Biol. Chem. 266:10967-10973(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY.

    Entry informationi

    Entry nameiRCEL_RHOSH
    AccessioniPrimary (citable) accession number: P0C0Y8
    Secondary accession number(s): P02954, Q9RFB9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3