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P0C0Y8 (RCEL_RHOSH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Reaction center protein L chain
Alternative name(s):
Photosynthetic reaction center L subunit
Gene names
Name:pufL
OrganismRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Taxonomic identifier1063 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.

Subunit structure

Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).

Subcellular location

Cellular chromatophore membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the reaction center PufL/M/PsbA/D family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 282281Reaction center protein L chain
PRO_0000090407

Regions

Topological domain2 – 3231Cytoplasmic Ref.7
Transmembrane33 – 5624Helical
Topological domain57 – 8327Periplasmic Ref.7
Transmembrane84 – 11229Helical
Topological domain113 – 1164Cytoplasmic Ref.7
Transmembrane117 – 13923Helical
Topological domain140 – 17132Periplasmic Ref.7
Transmembrane172 – 19928Helical
Topological domain200 – 22526Cytoplasmic Ref.7
Transmembrane226 – 25126Helical
Topological domain252 – 28231Periplasmic Ref.7

Sites

Metal binding1541Magnesium (bacteriochlorophyll b axial ligand)
Metal binding1741Magnesium (bacteriochlorophyll b axial ligand)
Metal binding1911Iron
Metal binding2311Iron
Binding site2171Quinone B

Secondary structure

................................................ 282
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0C0Y8 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 044F4D3AF085B136

FASTA28231,457
        10         20         30         40         50         60 
MALLSFERKY RVPGGTLVGG NLFDFWVGPF YVGFFGVATF FFAALGIILI AWSAVLQGTW 

        70         80         90        100        110        120 
NPQLISVYPP ALEYGLGGAP LAKGGLWQII TICATGAFVS WALREVEICR KLGIGYHIPF 

       130        140        150        160        170        180 
AFAFAILAYL TLVLFRPVMM GAWGYAFPYG IWTHLDWVSN TGYTYGNFHY NPAHMIAISF 

       190        200        210        220        230        240 
FFTNALALAL HGALVLSAAN PEKGKEMRTP DHEDTFFRDL VGYSIGTLGI HRLGLLLSLS 

       250        260        270        280 
AVFFSALCMI ITGTIWFDQW VDWWQWWVKL PWWANIPGGI NG 

« Hide

References

[1]"Primary structure of the L subunit of the reaction center from Rhodopseudomonas sphaeroides."
Williams J.C., Steiner L.A., Feher G., Simon M.I.
Proc. Natl. Acad. Sci. U.S.A. 81:7303-7307(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Towards the understanding of the function of Rb sphaeroides Y wild type reaction center: gene cloning, protein and detergent structures in the three-dimensional crystals."
Arnoux B., Ducruix A., Astier C., Picaud M., Roth M., Reiss-Husson F.
Biochimie 72:525-530(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Y.
[3]"Structure of the membrane-bound protein photosynthetic reaction center from Rhodobacter sphaeroides."
Chang C.-H., El-Kabbani O., Tiede D., Norris J., Schiffer M.
Biochemistry 30:5352-5360(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
[4]"Structure of the reaction center from Rhodobacter sphaeroides R-26: protein-cofactor (quinones and Fe2+) interactions."
Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.
Proc. Natl. Acad. Sci. U.S.A. 85:8487-8491(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
Strain: R-26.
[5]"Structure of the reaction center from Rhodobacter sphaeroides R-26: the protein subunits."
Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.
Proc. Natl. Acad. Sci. U.S.A. 84:6162-6166(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
Strain: R-26.
[6]"Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: monitoring the optical properties of the complex from bacterial cell to crystal."
McAuley-Hecht K.E., Fyfe P.K., Ridge J.P., Prince S.M., Hunter C.N., Isaacs N.W., Cogdell R.J., Jones M.R.
Biochemistry 37:4740-4750(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).
[7]"The use of gene fusions to examine the membrane topology of the L-subunit of the photosynthetic reaction center and of the cytochrome b subunit of the bc1 complex from Rhodobacter sphaeroides."
Yun C.H., Van Doren S.R., Crofts A.R., Gennis R.B.
J. Biol. Chem. 266:10967-10973(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M10206 Genomic DNA. Translation: AAA26177.1.
X63404 Genomic DNA. Translation: CAA44999.1.
PIRWNRFLS. S24212.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AIGX-ray2.60L/N2-282[»]
1AIJX-ray2.20L/R2-282[»]
1DS8X-ray2.50L/R2-282[»]
1DV3X-ray2.50L/R2-282[»]
1DV6X-ray2.50L/R2-282[»]
1E14X-ray2.70L2-282[»]
1E6DX-ray2.30L2-282[»]
1F6NX-ray2.80L2-282[»]
1FNPX-ray2.60L2-282[»]
1FNQX-ray2.60L2-282[»]
1JGWX-ray2.80L2-282[»]
1JGXX-ray3.01L2-282[»]
1JGYX-ray2.70L2-282[»]
1JGZX-ray2.70L2-282[»]
1JH0X-ray3.50L2-282[»]
1K6LX-ray3.10L2-282[»]
1K6NX-ray3.10L2-282[»]
1KBYX-ray2.50L2-282[»]
1L9BX-ray2.40L2-282[»]
1L9JX-ray3.25L/R2-282[»]
1M3XX-ray2.55L2-282[»]
1MPSX-ray2.55L2-282[»]
1OGVX-ray2.35L2-282[»]
1PCRX-ray2.65L2-282[»]
1PSSX-ray3.00L6-271[»]
1PSTX-ray3.00L6-271[»]
1QOVX-ray2.10L2-282[»]
1RG5X-ray2.50L2-282[»]
1RGNX-ray2.80L2-282[»]
1RQKX-ray2.70L2-282[»]
1RVJX-ray2.75L2-282[»]
1RY5X-ray2.10L2-282[»]
1RZHX-ray1.80L2-282[»]
1RZZX-ray2.40L/R2-282[»]
1S00X-ray2.60L/R2-282[»]
1UMXX-ray2.80L2-282[»]
1YF6X-ray2.25L2-282[»]
1YSTX-ray3.00L2-274[»]
1Z9JX-ray4.50A2-282[»]
1Z9KX-ray4.60A2-282[»]
2BNPX-ray2.70A2-282[»]
2BNSX-ray2.50A2-282[»]
2BOZX-ray2.40L2-282[»]
2GMRX-ray2.50L2-282[»]
2GNUX-ray2.20L2-281[»]
2HG3X-ray2.70L2-281[»]
2HG9X-ray2.45L2-281[»]
2HH1X-ray2.55L2-281[»]
2HHKX-ray2.50L2-281[»]
2HITX-ray2.75L2-281[»]
2HJ6X-ray3.00L2-281[»]
2J8CX-ray1.87L2-281[»]
2J8DX-ray2.07L2-281[»]
2JIYX-ray2.20L2-282[»]
2JJ0X-ray2.80L2-281[»]
2RCRX-ray3.10L2-282[»]
2UWSX-ray2.90L2-281[»]
2UWTX-ray2.50L2-281[»]
2UWUX-ray2.04L2-281[»]
2UWVX-ray2.13L2-281[»]
2UWWX-ray2.05L2-281[»]
2UX3X-ray2.50L2-281[»]
2UX4X-ray2.51L2-281[»]
2UX5X-ray2.21L2-281[»]
2UXJX-ray2.25L2-281[»]
2UXKX-ray2.31L2-281[»]
2UXLX-ray2.88L2-281[»]
2UXMX-ray2.70L2-281[»]
3DSYX-ray3.00L2-282[»]
3DTAX-ray3.20L2-282[»]
3DTRX-ray3.10L2-282[»]
3DTSX-ray3.10L2-282[»]
3DU2X-ray3.10L2-282[»]
3DU3X-ray2.80L2-282[»]
3DUQX-ray2.70L2-282[»]
3I4DX-ray2.01L2-282[»]
3V3YX-ray2.80L2-282[»]
3V3ZX-ray2.90L2-282[»]
3ZUMX-ray2.50L2-282[»]
3ZUWX-ray2.31L2-282[»]
4H99X-ray2.97L2-282[»]
4H9LX-ray2.77L2-282[»]
4HBHX-ray2.93L2-282[»]
4HBJX-ray2.74L2-282[»]
4IN7X-ray2.85L2-282[»]
4JC9X-ray7.78L1-282[»]
4JCBX-ray7.78L1-282[»]
4N7KX-ray2.85L2-282[»]
4RCRX-ray2.80L2-282[»]
ProteinModelPortalP0C0Y8.
SMRP0C0Y8. Positions 2-282.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60489N.

Protein family/group databases

TCDB3.E.2.1.1. the photosynthetic reaction center (prc) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.85.10. 2 hits.
InterProIPR005871. Photo_RC_L.
IPR000484. Photo_RC_L/M.
[Graphical view]
PfamPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSPR00256. REACTNCENTRE.
SUPFAMSSF81483. SSF81483. 1 hit.
TIGRFAMsTIGR01157. pufL. 1 hit.
PROSITEPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0C0Y8.

Entry information

Entry nameRCEL_RHOSH
AccessionPrimary (citable) accession number: P0C0Y8
Secondary accession number(s): P02954, Q9RFB9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references