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P0C0Y8

- RCEL_RHOSH

UniProt

P0C0Y8 - RCEL_RHOSH

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Protein

Reaction center protein L chain

Gene

pufL

Organism
Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi154 – 1541Magnesium (bacteriochlorophyll b axial ligand)
Metal bindingi174 – 1741Magnesium (bacteriochlorophyll b axial ligand)
Metal bindingi191 – 1911Iron
Binding sitei217 – 2171Quinone B
Metal bindingi231 – 2311Iron

GO - Molecular functioni

  1. bacteriochlorophyll binding Source: UniProtKB-KW
  2. electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. photosynthetic electron transport in photosystem II Source: InterPro
  2. protein-chromophore linkage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Bacteriochlorophyll, Chlorophyll, Chromophore, Iron, Magnesium, Metal-binding

Protein family/group databases

TCDBi3.E.2.1.1. the photosynthetic reaction center (prc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Reaction center protein L chain
Alternative name(s):
Photosynthetic reaction center L subunit
Gene namesi
Name:pufL
OrganismiRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Taxonomic identifieri1063 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane light-harvesting complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Reaction center

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 282281Reaction center protein L chainPRO_0000090407Add
BLAST

Interactioni

Subunit structurei

Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).

Protein-protein interaction databases

DIPiDIP-60489N.

Structurei

Secondary structure

1
282
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni5 – 73
Helixi8 – 103
Beta strandi17 – 193
Turni20 – 234
Beta strandi25 – 273
Beta strandi30 – 323
Helixi33 – 5725
Turni62 – 643
Helixi72 – 743
Helixi81 – 833
Helixi85 – 11228
Helixi117 – 13317
Helixi135 – 1406
Helixi143 – 1453
Helixi153 – 16311
Helixi168 – 1703
Helixi172 – 19928
Beta strandi202 – 2054
Helixi210 – 22112
Helixi227 – 25024
Turni253 – 2553
Helixi260 – 2634
Helixi265 – 2684
Turni271 – 2755
Beta strandi278 – 2803

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AIGX-ray2.60L/N2-282[»]
1AIJX-ray2.20L/R2-282[»]
1DS8X-ray2.50L/R2-282[»]
1DV3X-ray2.50L/R2-282[»]
1DV6X-ray2.50L/R2-282[»]
1E14X-ray2.70L2-282[»]
1E6DX-ray2.30L2-282[»]
1F6NX-ray2.80L2-282[»]
1FNPX-ray2.60L2-282[»]
1FNQX-ray2.60L2-282[»]
1JGWX-ray2.80L2-282[»]
1JGXX-ray3.01L2-282[»]
1JGYX-ray2.70L2-282[»]
1JGZX-ray2.70L2-282[»]
1JH0X-ray3.50L2-282[»]
1K6LX-ray3.10L2-282[»]
1K6NX-ray3.10L2-282[»]
1KBYX-ray2.50L2-282[»]
1L9BX-ray2.40L2-282[»]
1L9JX-ray3.25L/R2-282[»]
1M3XX-ray2.55L2-282[»]
1MPSX-ray2.55L2-282[»]
1OGVX-ray2.35L2-282[»]
1PCRX-ray2.65L2-282[»]
1PSSX-ray3.00L6-271[»]
1PSTX-ray3.00L6-271[»]
1QOVX-ray2.10L2-282[»]
1RG5X-ray2.50L2-282[»]
1RGNX-ray2.80L2-282[»]
1RQKX-ray2.70L2-282[»]
1RVJX-ray2.75L2-282[»]
1RY5X-ray2.10L2-282[»]
1RZHX-ray1.80L2-282[»]
1RZZX-ray2.40L/R2-282[»]
1S00X-ray2.60L/R2-282[»]
1UMXX-ray2.80L2-282[»]
1YF6X-ray2.25L2-282[»]
1YSTX-ray3.00L2-274[»]
1Z9JX-ray4.50A2-282[»]
1Z9KX-ray4.60A2-282[»]
2BNPX-ray2.70A2-282[»]
2BNSX-ray2.50A2-282[»]
2BOZX-ray2.40L2-282[»]
2GMRX-ray2.50L2-282[»]
2GNUX-ray2.20L2-282[»]
2HG3X-ray2.70L2-282[»]
2HG9X-ray2.45L2-282[»]
2HH1X-ray2.55L2-282[»]
2HHKX-ray2.50L2-282[»]
2HITX-ray2.75L2-282[»]
2HJ6X-ray3.00L2-282[»]
2J8CX-ray1.87L2-282[»]
2J8DX-ray2.07L2-282[»]
2JIYX-ray2.20L2-282[»]
2JJ0X-ray2.80L2-282[»]
2RCRX-ray3.10L2-282[»]
2UWSX-ray2.90L2-282[»]
2UWTX-ray2.50L2-282[»]
2UWUX-ray2.04L2-282[»]
2UWVX-ray2.13L2-282[»]
2UWWX-ray2.05L2-282[»]
2UX3X-ray2.50L2-282[»]
2UX4X-ray2.51L2-282[»]
2UX5X-ray2.21L2-282[»]
2UXJX-ray2.25L2-282[»]
2UXKX-ray2.31L2-282[»]
2UXLX-ray2.88L2-282[»]
2UXMX-ray2.70L2-282[»]
3DSYX-ray3.00L2-282[»]
3DTAX-ray3.20L2-282[»]
3DTRX-ray3.10L2-282[»]
3DTSX-ray3.10L2-282[»]
3DU2X-ray3.10L2-282[»]
3DU3X-ray2.80L2-282[»]
3DUQX-ray2.70L2-282[»]
3I4DX-ray2.01L2-282[»]
3V3YX-ray2.80L2-282[»]
3V3ZX-ray2.90L2-282[»]
3ZUMX-ray2.50L2-282[»]
3ZUWX-ray2.31L2-282[»]
4H99X-ray2.97L2-282[»]
4H9LX-ray2.77L2-282[»]
4HBHX-ray2.93L2-282[»]
4HBJX-ray2.74L2-282[»]
4IN7X-ray2.85L2-282[»]
4JC9X-ray7.78L1-282[»]
4JCBX-ray7.78L1-282[»]
4LWYX-ray2.90L1-282[»]
4N7KX-ray2.85L2-282[»]
4RCRX-ray2.80L2-282[»]
4TQQX-ray2.50L2-282[»]
ProteinModelPortaliP0C0Y8.
SMRiP0C0Y8. Positions 2-282.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0Y8.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 3231Cytoplasmic1 PublicationAdd
BLAST
Topological domaini57 – 8327Periplasmic1 PublicationAdd
BLAST
Topological domaini113 – 1164Cytoplasmic1 Publication
Topological domaini140 – 17132Periplasmic1 PublicationAdd
BLAST
Topological domaini200 – 22526Cytoplasmic1 PublicationAdd
BLAST
Topological domaini252 – 28231Periplasmic1 PublicationAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei33 – 5624HelicalAdd
BLAST
Transmembranei84 – 11229HelicalAdd
BLAST
Transmembranei117 – 13923HelicalAdd
BLAST
Transmembranei172 – 19928HelicalAdd
BLAST
Transmembranei226 – 25126HelicalAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.85.10. 2 hits.
InterProiIPR005871. Photo_RC_L.
IPR000484. Photo_RC_L/M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01157. pufL. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C0Y8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALLSFERKY RVPGGTLVGG NLFDFWVGPF YVGFFGVATF FFAALGIILI
60 70 80 90 100
AWSAVLQGTW NPQLISVYPP ALEYGLGGAP LAKGGLWQII TICATGAFVS
110 120 130 140 150
WALREVEICR KLGIGYHIPF AFAFAILAYL TLVLFRPVMM GAWGYAFPYG
160 170 180 190 200
IWTHLDWVSN TGYTYGNFHY NPAHMIAISF FFTNALALAL HGALVLSAAN
210 220 230 240 250
PEKGKEMRTP DHEDTFFRDL VGYSIGTLGI HRLGLLLSLS AVFFSALCMI
260 270 280
ITGTIWFDQW VDWWQWWVKL PWWANIPGGI NG
Length:282
Mass (Da):31,457
Last modified:January 23, 2007 - v2
Checksum:i044F4D3AF085B136
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10206 Genomic DNA. Translation: AAA26177.1.
X63404 Genomic DNA. Translation: CAA44999.1.
PIRiS24212. WNRFLS.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10206 Genomic DNA. Translation: AAA26177.1 .
X63404 Genomic DNA. Translation: CAA44999.1 .
PIRi S24212. WNRFLS.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AIG X-ray 2.60 L/N 2-282 [» ]
1AIJ X-ray 2.20 L/R 2-282 [» ]
1DS8 X-ray 2.50 L/R 2-282 [» ]
1DV3 X-ray 2.50 L/R 2-282 [» ]
1DV6 X-ray 2.50 L/R 2-282 [» ]
1E14 X-ray 2.70 L 2-282 [» ]
1E6D X-ray 2.30 L 2-282 [» ]
1F6N X-ray 2.80 L 2-282 [» ]
1FNP X-ray 2.60 L 2-282 [» ]
1FNQ X-ray 2.60 L 2-282 [» ]
1JGW X-ray 2.80 L 2-282 [» ]
1JGX X-ray 3.01 L 2-282 [» ]
1JGY X-ray 2.70 L 2-282 [» ]
1JGZ X-ray 2.70 L 2-282 [» ]
1JH0 X-ray 3.50 L 2-282 [» ]
1K6L X-ray 3.10 L 2-282 [» ]
1K6N X-ray 3.10 L 2-282 [» ]
1KBY X-ray 2.50 L 2-282 [» ]
1L9B X-ray 2.40 L 2-282 [» ]
1L9J X-ray 3.25 L/R 2-282 [» ]
1M3X X-ray 2.55 L 2-282 [» ]
1MPS X-ray 2.55 L 2-282 [» ]
1OGV X-ray 2.35 L 2-282 [» ]
1PCR X-ray 2.65 L 2-282 [» ]
1PSS X-ray 3.00 L 6-271 [» ]
1PST X-ray 3.00 L 6-271 [» ]
1QOV X-ray 2.10 L 2-282 [» ]
1RG5 X-ray 2.50 L 2-282 [» ]
1RGN X-ray 2.80 L 2-282 [» ]
1RQK X-ray 2.70 L 2-282 [» ]
1RVJ X-ray 2.75 L 2-282 [» ]
1RY5 X-ray 2.10 L 2-282 [» ]
1RZH X-ray 1.80 L 2-282 [» ]
1RZZ X-ray 2.40 L/R 2-282 [» ]
1S00 X-ray 2.60 L/R 2-282 [» ]
1UMX X-ray 2.80 L 2-282 [» ]
1YF6 X-ray 2.25 L 2-282 [» ]
1YST X-ray 3.00 L 2-274 [» ]
1Z9J X-ray 4.50 A 2-282 [» ]
1Z9K X-ray 4.60 A 2-282 [» ]
2BNP X-ray 2.70 A 2-282 [» ]
2BNS X-ray 2.50 A 2-282 [» ]
2BOZ X-ray 2.40 L 2-282 [» ]
2GMR X-ray 2.50 L 2-282 [» ]
2GNU X-ray 2.20 L 2-282 [» ]
2HG3 X-ray 2.70 L 2-282 [» ]
2HG9 X-ray 2.45 L 2-282 [» ]
2HH1 X-ray 2.55 L 2-282 [» ]
2HHK X-ray 2.50 L 2-282 [» ]
2HIT X-ray 2.75 L 2-282 [» ]
2HJ6 X-ray 3.00 L 2-282 [» ]
2J8C X-ray 1.87 L 2-282 [» ]
2J8D X-ray 2.07 L 2-282 [» ]
2JIY X-ray 2.20 L 2-282 [» ]
2JJ0 X-ray 2.80 L 2-282 [» ]
2RCR X-ray 3.10 L 2-282 [» ]
2UWS X-ray 2.90 L 2-282 [» ]
2UWT X-ray 2.50 L 2-282 [» ]
2UWU X-ray 2.04 L 2-282 [» ]
2UWV X-ray 2.13 L 2-282 [» ]
2UWW X-ray 2.05 L 2-282 [» ]
2UX3 X-ray 2.50 L 2-282 [» ]
2UX4 X-ray 2.51 L 2-282 [» ]
2UX5 X-ray 2.21 L 2-282 [» ]
2UXJ X-ray 2.25 L 2-282 [» ]
2UXK X-ray 2.31 L 2-282 [» ]
2UXL X-ray 2.88 L 2-282 [» ]
2UXM X-ray 2.70 L 2-282 [» ]
3DSY X-ray 3.00 L 2-282 [» ]
3DTA X-ray 3.20 L 2-282 [» ]
3DTR X-ray 3.10 L 2-282 [» ]
3DTS X-ray 3.10 L 2-282 [» ]
3DU2 X-ray 3.10 L 2-282 [» ]
3DU3 X-ray 2.80 L 2-282 [» ]
3DUQ X-ray 2.70 L 2-282 [» ]
3I4D X-ray 2.01 L 2-282 [» ]
3V3Y X-ray 2.80 L 2-282 [» ]
3V3Z X-ray 2.90 L 2-282 [» ]
3ZUM X-ray 2.50 L 2-282 [» ]
3ZUW X-ray 2.31 L 2-282 [» ]
4H99 X-ray 2.97 L 2-282 [» ]
4H9L X-ray 2.77 L 2-282 [» ]
4HBH X-ray 2.93 L 2-282 [» ]
4HBJ X-ray 2.74 L 2-282 [» ]
4IN7 X-ray 2.85 L 2-282 [» ]
4JC9 X-ray 7.78 L 1-282 [» ]
4JCB X-ray 7.78 L 1-282 [» ]
4LWY X-ray 2.90 L 1-282 [» ]
4N7K X-ray 2.85 L 2-282 [» ]
4RCR X-ray 2.80 L 2-282 [» ]
4TQQ X-ray 2.50 L 2-282 [» ]
ProteinModelPortali P0C0Y8.
SMRi P0C0Y8. Positions 2-282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60489N.

Protein family/group databases

TCDBi 3.E.2.1.1. the photosynthetic reaction center (prc) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P0C0Y8.

Family and domain databases

Gene3Di 1.20.85.10. 2 hits.
InterProi IPR005871. Photo_RC_L.
IPR000484. Photo_RC_L/M.
[Graphical view ]
Pfami PF00124. Photo_RC. 1 hit.
[Graphical view ]
PRINTSi PR00256. REACTNCENTRE.
SUPFAMi SSF81483. SSF81483. 1 hit.
TIGRFAMsi TIGR01157. pufL. 1 hit.
PROSITEi PS00244. REACTION_CENTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of the L subunit of the reaction center from Rhodopseudomonas sphaeroides."
    Williams J.C., Steiner L.A., Feher G., Simon M.I.
    Proc. Natl. Acad. Sci. U.S.A. 81:7303-7307(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Towards the understanding of the function of Rb sphaeroides Y wild type reaction center: gene cloning, protein and detergent structures in the three-dimensional crystals."
    Arnoux B., Ducruix A., Astier C., Picaud M., Roth M., Reiss-Husson F.
    Biochimie 72:525-530(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Y.
  3. "Structure of the membrane-bound protein photosynthetic reaction center from Rhodobacter sphaeroides."
    Chang C.-H., El-Kabbani O., Tiede D., Norris J., Schiffer M.
    Biochemistry 30:5352-5360(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
  4. "Structure of the reaction center from Rhodobacter sphaeroides R-26: protein-cofactor (quinones and Fe2+) interactions."
    Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.
    Proc. Natl. Acad. Sci. U.S.A. 85:8487-8491(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    Strain: R-26.
  5. "Structure of the reaction center from Rhodobacter sphaeroides R-26: the protein subunits."
    Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.
    Proc. Natl. Acad. Sci. U.S.A. 84:6162-6166(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    Strain: R-26.
  6. "Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: monitoring the optical properties of the complex from bacterial cell to crystal."
    McAuley-Hecht K.E., Fyfe P.K., Ridge J.P., Prince S.M., Hunter C.N., Isaacs N.W., Cogdell R.J., Jones M.R.
    Biochemistry 37:4740-4750(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).
  7. "The use of gene fusions to examine the membrane topology of the L-subunit of the photosynthetic reaction center and of the cytochrome b subunit of the bc1 complex from Rhodobacter sphaeroides."
    Yun C.H., Van Doren S.R., Crofts A.R., Gennis R.B.
    J. Biol. Chem. 266:10967-10973(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.

Entry informationi

Entry nameiRCEL_RHOSH
AccessioniPrimary (citable) accession number: P0C0Y8
Secondary accession number(s): P02954, Q9RFB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3