P0C0Y7 (RCEH_RHOSH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Reaction center protein H chain

Alternative name(s):
Photosynthetic reaction center H subunit

Gene names

Name:

puhA

Organism

Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)

Taxonomic identifier

1063 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
Cofactor	Binds 4 bacteriochlorophylls per trimer. Binds 2 bacteriopheophytins per trimer. Binds 1 iron ion per trimer. Binds 4 magnesium ions per trimer. Binds 2 ubiquinone per trimer.
Subunit structure	Heterotrimer composed of subunits L, M, and H.
Subcellular location	Cellular chromatophore membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the reaction center PuhA family.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Membrane Reaction center
Domain	Transmembrane Transmembrane helix
Ligand	Bacteriochlorophyll Chlorophyll Chromophore
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis, light reaction Inferred from electronic annotation. Source: InterPro protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane light-harvesting complex Inferred from electronic annotation. Source: InterPro plasma membrane-derived chromatophore membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	bacteriochlorophyll binding Inferred from electronic annotation. Source: UniProtKB-KW electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 260

260

Reaction center protein H chain

PRO_0000090394

Regions

Topological domain

1 – 11

11

Periplasmic

Transmembrane

12 – 31

20

Helical

Topological domain

32 – 260

229

Cytoplasmic

Secondary structure

1

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260

Helix Strand Turn

Details...

Turn

6 – 8

3

Helix

12 – 34

23

Beta strand

37 – 39

3

Beta strand

40 – 43

4

Beta strand

47 – 49

3

Beta strand

50 – 52

3

Beta strand

56 – 58

3

Beta strand

62 – 66

5

Helix

67 – 69

3

Beta strand

71 – 76

6

Beta strand

87 – 93

7

Beta strand

98 – 102

5

Helix

104 – 107

4

Helix

110 – 112

3

Beta strand

127 – 133

7

Helix

134 – 136

3

Beta strand

137 – 139

3

Beta strand

141 – 145

5

Helix

148 – 150

3

Beta strand

152 – 155

4

Beta strand

156 – 158

3

Beta strand

160 – 170

11

Turn

171 – 174

4

Beta strand

175 – 182

8

Beta strand

184 – 186

3

Beta strand

188 – 192

5

Helix

193 – 195

3

Beta strand

197 – 199

3

Beta strand

203 – 207

5

Helix

210 – 212

3

Turn

213 – 215

3

Beta strand

220 – 223

4

Helix

227 – 243

17

Helix

245 – 247

3

Beta strand

248 – 250

3

Helix

251 – 254

4

Sequences

	Sequence		Length	Mass (Da)
	P0C0Y7 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 7E0B913B63D4DEB0	FASTA	260	28,035
	10 20 30 40 50 60 MVGVTAFGNF DLASLAIYSF WIFLAGLIYY LQTENMREGY PLENEDGTPA ANQGPFPLPK 70 80 90 100 110 120 PKTFILPHGR GTLTVPGPES EDRPIALART AVSEGFPHAP TGDPMKDGVG PASWVARRDL 130 140 150 160 170 180 PELDGHGHNK IKPMKAAAGF HVSAGKNPIG LPVRGCDLEI AGKVVDIWVD IPEQMARFLE 190 200 210 220 230 240 VELKDGSTRL LPMQMVKVQS NRVHVNALSS DLFAGIPTIK SPTEVTLLEE DKICGYVAGG 250 260 LMYAAPKRKS VVAAMLAEYA

References

[1]	"Primary structure of the reaction center from Rhodopseudomonas sphaeroides." Williams J.C., Steiner L.A., Feher G. Proteins 1:312-325(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Picaud M. Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Y.
[3]	"Structure of the membrane-bound protein photosynthetic reaction center from Rhodobacter sphaeroides." Chang C.-H., El-Kabbani O., Tiede D., Norris J., Schiffer M. Biochemistry 30:5352-5360(1991) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
[4]	"Structure of the reaction center from Rhodobacter sphaeroides R-26: protein-cofactor (quinones and Fe2+) interactions." Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C. Proc. Natl. Acad. Sci. U.S.A. 85:8487-8491(1988) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS). Strain: R-26.
[5]	"Structure of the reaction center from Rhodobacter sphaeroides R-26: the protein subunits." Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C. Proc. Natl. Acad. Sci. U.S.A. 84:6162-6166(1987) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS). Strain: R-26.
[6]	"Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: monitoring the optical properties of the complex from bacterial cell to crystal." McAuley-Hecht K.E., Fyfe P.K., Ridge J.P., Prince S.M., Hunter C.N., Isaacs N.W., Cogdell R.J., Jones M.R. Biochemistry 37:4740-4750(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M18412 Genomic DNA. Translation: AAA26176.1.
X63378 Genomic DNA. Translation: CAA44976.1.

PIR

A26538.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AIG	X-ray	2.60	H/P	1-260	[»]
1AIJ	X-ray	2.20	H/T	1-260	[»]
1DS8	X-ray	2.50	H/T	1-260	[»]
1DV3	X-ray	2.50	H/T	1-260	[»]
1DV6	X-ray	2.50	H/T	1-260	[»]
1E14	X-ray	2.70	H	1-260	[»]
1E6D	X-ray	2.30	H	1-260	[»]
1F6N	X-ray	2.80	H	1-260	[»]
1FNP	X-ray	2.60	H	1-260	[»]
1FNQ	X-ray	2.60	H	1-260	[»]
1JGW	X-ray	2.80	H	1-260	[»]
1JGX	X-ray	3.01	H	1-260	[»]
1JGY	X-ray	2.70	H	1-260	[»]
1JGZ	X-ray	2.70	H	1-260	[»]
1JH0	X-ray	3.50	H	1-260	[»]
1K6L	X-ray	3.10	H	1-260	[»]
1K6N	X-ray	3.10	H	1-260	[»]
1KBY	X-ray	2.50	H	1-260	[»]
1L9B	X-ray	2.40	H	1-260	[»]
1L9J	X-ray	3.25	H/T	1-260	[»]
1M3X	X-ray	2.55	H	1-260	[»]
1MPS	X-ray	2.55	H	1-260	[»]
1OGV	X-ray	2.35	H	11-260	[»]
1PCR	X-ray	2.65	H	1-260	[»]
1PSS	X-ray	3.00	H	12-248	[»]
1PST	X-ray	3.00	H	12-248	[»]
1QOV	X-ray	2.10	H	1-260	[»]
1RG5	X-ray	2.50	H	1-260	[»]
1RGN	X-ray	2.80	H	1-260	[»]
1RQK	X-ray	2.70	H	1-260	[»]
1RVJ	X-ray	2.75	H	1-260	[»]
1RY5	X-ray	2.10	H	1-260	[»]
1RZH	X-ray	1.80	H	1-260	[»]
1RZZ	X-ray	2.40	H/T	1-260	[»]
1S00	X-ray	2.60	H/T	1-260	[»]
1UMX	X-ray	2.80	H	1-260	[»]
1YF6	X-ray	2.25	H	1-260	[»]
1YST	X-ray	3.00	H	1-260	[»]
1Z9J	X-ray	4.50	C	1-260	[»]
1Z9K	X-ray	4.60	C	1-260	[»]
2BNP	X-ray	2.70	C	1-260	[»]
2BNS	X-ray	2.50	C	1-260	[»]
2BOZ	X-ray	2.40	H	1-260	[»]
2GMR	X-ray	2.50	H	1-260	[»]
2GNU	X-ray	2.20	H	11-245	[»]
2HG3	X-ray	2.70	H	1-260	[»]
2HG9	X-ray	2.45	H	1-260	[»]
2HH1	X-ray	2.55	H	1-260	[»]
2HHK	X-ray	2.50	H	1-260	[»]
2HIT	X-ray	2.75	H	1-260	[»]
2HJ6	X-ray	3.00	H	1-260	[»]
2J8C	X-ray	1.87	H	1-260	[»]
2J8D	X-ray	2.07	H	1-260	[»]
2JIY	X-ray	2.20	H	1-260	[»]
2JJ0	X-ray	2.80	H	1-260	[»]
2RCR	X-ray	3.10	H	1-260	[»]
2UWS	X-ray	2.90	H	1-260	[»]
2UWT	X-ray	2.50	H	1-260	[»]
2UWU	X-ray	2.04	H	1-260	[»]
2UWV	X-ray	2.13	H	1-260	[»]
2UWW	X-ray	2.05	H	1-260	[»]
2UX3	X-ray	2.50	H	1-260	[»]
2UX4	X-ray	2.51	H	1-260	[»]
2UX5	X-ray	2.21	H	1-260	[»]
2UXJ	X-ray	2.25	H	1-260	[»]
2UXK	X-ray	2.31	H	1-260	[»]
2UXL	X-ray	2.88	H	1-260	[»]
2UXM	X-ray	2.70	H	1-260	[»]
3DSY	X-ray	3.00	H	1-260	[»]
3DTA	X-ray	3.20	H	1-260	[»]
3DTR	X-ray	3.10	H	1-260	[»]
3DTS	X-ray	3.10	H	1-260	[»]
3DU2	X-ray	3.10	H	1-260	[»]
3DU3	X-ray	2.80	H	1-260	[»]
3DUQ	X-ray	2.70	H	1-260	[»]
3I4D	X-ray	2.01	H	1-260	[»]
3V3Y	X-ray	2.80	H	10-250	[»]
3V3Z	X-ray	2.90	H	10-250	[»]
3ZUM	X-ray	2.50	H	1-260	[»]
3ZUW	X-ray	2.31	H	1-260	[»]
4H99	X-ray	2.97	H	1-260	[»]
4H9L	X-ray	2.77	H	1-260	[»]
4HBH	X-ray	2.93	H	1-260	[»]
4HBJ	X-ray	2.74	H	1-260	[»]
4RCR	X-ray	2.80	H	1-260	[»]

ProteinModelPortal

P0C0Y7.

SMR

P0C0Y7. Positions 1-260.

ModBase

Search...

Protein family/group databases

TCDB

3.E.2.1.1. photosynthetic reaction center (PRC) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.90.50.10. 1 hit.
4.10.540.10. 1 hit.

InterPro

IPR014747. Bac_photo_RC_H_C.
IPR005652. Photo_RC_H.
IPR015810. Photo_RC_H_N.
IPR027275. PRC-brl_dom.
IPR011033. PRC_barrel-like.
[Graphical view]

Pfam

PF05239. PRC. 1 hit.
PF03967. PRCH. 1 hit.
[Graphical view]

SUPFAM

SSF50346. PRCH_cytoplasmic. 1 hit.

TIGRFAMs

TIGR01150. puhA. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0C0Y7.

Entry information

Entry name

RCEH_RHOSH

Accession

Primary (citable) accession number: P0C0Y7
Secondary accession number(s): P11846, Q9RFD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 24, 2006
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program