P0C0Y5 (MTDH_DAVTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Probable NADP-dependent mannitol dehydrogenase Short name=MtDH EC=1.1.1.138 Alternative name(s): Mannitol 2-dehydrogenase [NADP+] Allergen=Cla h 8
Organism	Davidiella tassiana (Mycosphaerella tassiana) (Cladosporium herbarum)
Taxonomic identifier	29918 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Dothideomycetidae › Capnodiales › Davidiellaceae › Davidiella

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-mannitol + NADP+ = D-fructose + NADPH.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Disease	Allergen
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Molecular function	mannitol 2-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 267

267

Probable NADP-dependent mannitol dehydrogenase

PRO_0000054728

Regions

Nucleotide binding

30 – 55

26

NADP By similarity

Nucleotide binding

161 – 180

20

NADP By similarity

Sites

Active site

175

1

Proton acceptor By similarity

Binding site

160

1

Substrate By similarity

Secondary structure

1

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267

Helix Strand Turn

Details...

Helix

12 – 16

5

Beta strand

22 – 25

4

Turn

37 – 39

3

Beta strand

48 – 51

4

Beta strand

53 – 56

4

Helix

61 – 70

10

Helix

85 – 98

14

Beta strand

103 – 107

5

Helix

122 – 132

11

Helix

137 – 142

6

Helix

146 – 149

4

Beta strand

155 – 158

4

Beta strand

168 – 170

3

Helix

173 – 179

7

Helix

183 – 192

10

Turn

193 – 196

4

Beta strand

199 – 203

5

Helix

212 – 214

3

Helix

217 – 224

8

Helix

235 – 237

3

Helix

242 – 246

5

Beta strand

257 – 259

3

Sequences

	Sequence		Length	Mass (Da)
	P0C0Y5 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 4CF5EA012D71D446	FASTA	267	28,464
	10 20 30 40 50 60 MPGQQATKHE SLLDQLSLKG KVVVVTGASG PKGMGIEAAR GCAEMGAAVA ITYASRAQGA 70 80 90 100 110 120 EENVKELEKT YGIKAKAYKC QVDSYESCEK LVKDVVADFG QIDAFIANAG ATADSGILDG 130 140 150 160 170 180 SVEAWNHVVQ VDLNGTFHCA KAVGHHFKER GTGSLVITAS MSGHIANFPQ EQTSYNVAKA 190 200 210 220 230 240 GCIHMARSLA NEWRDFARVN SISPGYIDTG LSDFVPKETQ QLWHSMIPMG RDGLAKELKG 250 260 AYVYFASDAS TYTTGADLLI DGGYTTR

References

[1]	"Cladosporium herbarum NADP-dependent mannitol dehydrogenase, a major allergen." Denk U., Schneider P., Breitenbach M., Richter K., Radauer C., Teige M., Simon-Nobbe B. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 280202-Berlin.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY191816 mRNA. Translation: AAO91801.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GDF	X-ray	2.50	A/B/C/D	1-267	[»]
3GDG	X-ray	2.30	A/B/C/D	1-267	[»]

ProteinModelPortal

P0C0Y5.

ModBase

Search...

Protein family/group databases

Allergome

2485. Cla h 8.
3207. Cla h 8.0101.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

1.1.1.138. 7286.

Family and domain databases

InterPro

IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR00081. GDHRDH.

PROSITE

PS00061. ADH_SHORT. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MTDH_DAVTA

Accession

Primary (citable) accession number: P0C0Y5
Secondary accession number(s): Q2TV79

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 24, 2006
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program