Reviewed, UniProtKB/Swiss-Prot P0C0Y4 (MTDH_ALTAL)

Names and origin

Protein names	Recommended name:     Probable NADP-dependent mannitol dehydrogenase       Short name=MtDH     EC=1.1.1.138 Alternative name(s):     Mannitol 2-dehydrogenase [NADP+]     Allergen=Alt a 8
Organism	Alternaria alternata (Alternaria rot fungus)
Taxonomic identifier	5599 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporaceae › mitosporic Pleosporaceae › Alternaria › Alternaria alternata group

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-mannitol + NADP+ = D-fructose + NADPH.
Subunit structure	Homotetramer By similarity.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Disease	Allergen
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro mannitol 2-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Probable NADP-dependent mannitol dehydrogenase		PRO_0000054727
Regions
	Nucleotide binding	29 – 54	26	NADP By similarity
	Nucleotide binding	160 – 179	20	NADP By similarity
Sites
	Active site	174	1	Proton acceptor By similarity
	Binding site	159	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C0Y4-1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 96CC180295A38901	FASTA	266	28,735
	10 20 30 40 50 60 MPISVPQATE LKDLFSLKGK VVIVTGASGP TGIGTEAARG CAEYGADLAI TYNSRAEGAE 70 80 90 100 110 120 KNAKEMSEKY GVKVKAYKCQ VNEYAQCEKL VQDVIKDFGK VDVFIANAGK TADNGILDAT 130 140 150 160 170 180 VEQWNEVIQT DLTGTFNCAR AVGLHFRERK TGSLVITSSM SGHIANFPQE QASYNVAKAG 190 200 210 220 230 240 CIHLAKSLAN EWRDFARVNS ISPGYIDTGL SDFVPQDIQK LWHSMIPMGR DAKATELKGA 250 260 YVYFASDASS YCTGSDLLID GGYCVR

References

[1]	"Alternaria alternata mannitol metabolism in plant-pathogen interactions." Velez H., Ehrenshaft M., Daub M.E. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF541874 Genomic DNA. Translation: AAN28666.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.138. 74336.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTDH_ALTAL

Accession

Primary (citable) accession number: P0C0Y4
Secondary accession number(s): Q2TV80, Q8J231

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	October 31, 2006
Last modified:	November 24, 2009
This is version 17 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)