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Protein

Probable NADP-dependent mannitol dehydrogenase

Gene
N/A
Organism
Alternaria alternata (Alternaria rot fungus) (Torula alternata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-mannitol + NADP+ = D-fructose + NADPH.1 Publication

Kineticsi

  1. KM=474 mM for D-fructose1 Publication
  2. KM=18.7 µM for NADPH1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei159 – 1591SubstrateBy similarity
    Active sitei174 – 1741Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 5426NADPBy similarityAdd
    BLAST
    Nucleotide bindingi160 – 17920NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BRENDAi1.1.1.138. 267.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable NADP-dependent mannitol dehydrogenase (EC:1.1.1.138)
    Short name:
    MtDH
    Alternative name(s):
    Mannitol 2-dehydrogenase [NADP(+)]
    Allergen: Alt a 8
    OrganismiAlternaria alternata (Alternaria rot fungus) (Torula alternata)
    Taxonomic identifieri5599 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePleosporaceaeAlternariaAlternaria alternata group

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. Binds to IgE.1 Publication

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei22. Alt a 8.
    3065. Alt a 8.0101.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 266266Probable NADP-dependent mannitol dehydrogenasePRO_0000054727Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0C0Y4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR002347. SDR_fam.
    [Graphical view]
    PANTHERiPTHR24322. PTHR24322. 2 hits.
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMiSSF51735. SSF51735. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0C0Y4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPISVPQATE LKDLFSLKGK VVIVTGASGP TGIGTEAARG CAEYGADLAI
    60 70 80 90 100
    TYNSRAEGAE KNAKEMSEKY GVKVKAYKCQ VNEYAQCEKL VQDVIKDFGK
    110 120 130 140 150
    VDVFIANAGK TADNGILDAT VEQWNEVIQT DLTGTFNCAR AVGLHFRERK
    160 170 180 190 200
    TGSLVITSSM SGHIANFPQE QASYNVAKAG CIHLAKSLAN EWRDFARVNS
    210 220 230 240 250
    ISPGYIDTGL SDFVPQDIQK LWHSMIPMGR DAKATELKGA YVYFASDASS
    260
    YCTGSDLLID GGYCVR
    Length:266
    Mass (Da):28,735
    Last modified:October 31, 2006 - v2
    Checksum:i96CC180295A38901
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41S → T in AAO91800 (PubMed:17177674).Curated

    Mass spectrometryi

    Molecular mass is 28618 Da from positions 1 - 266. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY191815 mRNA. Translation: AAO91800.1.
    AF541874 Genomic DNA. Translation: AAN28666.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY191815 mRNA. Translation: AAO91800.1.
    AF541874 Genomic DNA. Translation: AAN28666.1.

    3D structure databases

    ProteinModelPortaliP0C0Y4.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    Allergomei22. Alt a 8.
    3065. Alt a 8.0101.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    BRENDAi1.1.1.138. 267.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR002347. SDR_fam.
    [Graphical view]
    PANTHERiPTHR24322. PTHR24322. 2 hits.
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    1. "Alternaria alternata NADP-dependent mannitol dehydrogenase is an important fungal allergen."
      Schneider P.B., Denk U., Breitenbach M., Richter K., Schmid-Grendelmeier P., Nobbe S., Himly M., Mari A., Ebner C., Simon-Nobbe B.
      Clin. Exp. Allergy 36:1513-1524(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MASS SPECTROMETRY, ALLERGEN.
      Strain: 08-0203-Berlin.
    2. "Alternaria alternata mannitol metabolism in plant-pathogen interactions."
      Velez H., Ehrenshaft M., Daub M.E.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A5.

    Entry informationi

    Entry nameiMTDH_ALTAL
    AccessioniPrimary (citable) accession number: P0C0Y4
    Secondary accession number(s): Q2TV80, Q8J231
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2006
    Last sequence update: October 31, 2006
    Last modified: May 11, 2016
    This is version 46 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.