P0C0Y1 (LHB1_RHOSH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Light-harvesting protein B-875 beta chain

Alternative name(s):
Antenna pigment protein beta chain
LH-3A

Gene names

Name:

pufB

Organism

Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)

Taxonomic identifier

1063 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	49 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Antenna complexes are light-harvesting systems, which transfer the excitation energy to the reaction centers.
Subunit structure	The core complex is formed by different alpha and beta chains, binding bacteriochlorophyll molecules, and arranged most probably in tetrameric structures disposed around the reaction center. The non-pigmented gamma chains may constitute additional components.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the antena complex beta subunit family.

Ontologies

Keywords
Cellular component	Antenna complex Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Bacteriochlorophyll Chlorophyll Chromophore Magnesium Metal-binding
Molecular function	Light-harvesting polypeptide
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	photosynthesis, light reaction Inferred from electronic annotation. Source: InterPro protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane light-harvesting complex Inferred from electronic annotation. Source: InterPro
Molecular_function	bacteriochlorophyll binding Inferred from electronic annotation. Source: UniProtKB-KW electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 49

48

Light-harvesting protein B-875 beta chain

PRO_0000099833

Regions

Topological domain

2 – 27

26

Cytoplasmic

Transmembrane

28 – 45

18

Helical; Signal-anchor for type II membrane protein

Topological domain

46 – 49

4

Periplasmic

Sites

Metal binding

21

1

Magnesium (bacteriochlorophyll axial ligand)

Metal binding

39

1

Magnesium (bacteriochlorophyll axial ligand)

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

49

Helix Strand Turn

Details...

Helix

5 – 8

4

Helix

9 – 20

12

Helix

22 – 31

10

Helix

34 – 44

11

Turn

46 – 48

3

Sequences

	Sequence		Length	Mass (Da)
	P0C0Y1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8C5B2F647A157582	FASTA	49	5,572
	10 20 30 40 MADKSDLGYT GLTDEQAQEL HSVYMSGLWP FSAVAIVAHL AVYIWRPWF

References

[1]	"The light-harvesting polypeptides of Rhodopseudomonas sphaeroides R-26.1. I. Isolation, purification and sequence analyses." Theiler R., Suter F., Wiemken V., Zuber H. Hoppe-Seyler's Z. Physiol. Chem. 365:703-719(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-49. Strain: R-26.1.
[2]	"The solution structure of Rhodobacter sphaeroides LH1beta reveals two helical domains separated by a more flexible region: structural consequences for the LH1 complex." Conroy M.J., Westerhuis W.H.J., Parkes-Loach P.S., Loach P.A., Hunter C.N., Williamson M.P. J. Mol. Biol. 298:83-94(2000) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR. Strain: DD13.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

LBRFAS. B27760.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DX7	NMR	-	A	2-49	[»]
1JO5	NMR	-	A	2-49	[»]

ProteinModelPortal

P0C0Y1.

SMR

P0C0Y1. Positions 2-49.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000066. Antenna_a/b.
IPR002362. Antenna_beta.
IPR023623. Antenna_beta_CS.
[Graphical view]

Pfam

PF00556. LHC. 1 hit.
[Graphical view]

PIRSF

PIRSF002900. Antenna_beta. 1 hit.

PRINTS

PR00674. LIGHTHARVSTB.

SUPFAM

SSF56918. Antenna_a/b. 1 hit.

PROSITE

PS00969. ANTENNA_COMP_BETA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0C0Y1.

Entry information

Entry name

LHB1_RHOSH

Accession

Primary (citable) accession number: P0C0Y1
Secondary accession number(s): P02951

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program