P0C0X1 (DHPS1_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydropteroate synthase 1
Short name=DHPS 1
EC=2.5.1.15

Alternative name(s):
Dihydropteroate pyrophosphorylase 1

Gene names

Name:	folP1
Synonyms:	folP
Ordered Locus Names:	ML0224
ORF Names:	MLCB2548.07c

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide By similarity.
Catalytic activity	(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.
Cofactor	Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHPS family. Contains 1 pterin-binding domain.

Ontologies

Keywords
Biological process	Antibiotic resistance Folate biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydropteroate synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Dihydropteroate synthase 1		PRO_0000168213
Regions
	Domain	6 – 265	260	Pterin-binding
	Region	53 – 54	2	Substrate binding By similarity
Sites
	Metal binding	13	1	Magnesium By similarity
	Binding site	21	1	Substrate By similarity
	Binding site	86	1	Substrate By similarity
	Binding site	105	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	253	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity
Experimental info
	Sequence conflict	53	1	T → A in BAA84081. Ref.1
	Sequence conflict	53	1	T → I in BAA84080. Ref.1
	Sequence conflict	53	1	T → I in BAA84079. Ref.1
	Sequence conflict	55	1	P → L in BAA84078. Ref.1
	Sequence conflict	55	1	P → L in BAA84077. Ref.1
	Sequence conflict	55	1	P → L in BAA84076. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0C0X1 [UniParc]. Last modified January 10, 2006. Version 1. Checksum: A576FF46C16AC826	FASTA	284	29,448
	10 20 30 40 50 60 MSLAPVQVIG VLNVTDNSFS DGGRYLDPDD AVQHGLAMVA EGAAIVDVGG ESTRPGAIRT 70 80 90 100 110 120 DPRVELSRIV PVVKELAAQG ITVSIDTTRA DVARAALQSG ARIVNDVSGG RADPAMAPLV 130 140 150 160 170 180 AEAGVAWVLM HWRLMSAERP YEAPNYRDVV AEVRADLLAG VDQAVAAGVD PGSLVIDPGL 190 200 210 220 230 240 GFAKTGQHNW ALLNALPELV ATGVPILLGA SRKRFLGRLL AGADGAVRPP DGRETATAVI 250 260 270 280 SALAALHGAW GVRVHDVRAS VDALKVVGAW LHAGPQIEKV RCDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Diaminodiphenylsulfone resistance of Mycobacterium leprae due to mutations in the dihydropteroate synthase gene." Kai M., Matsuoka M., Nakata N., Maeda S., Gidoh M., Maeda Y., Hashimoto K., Kobayashi K., Kashiwabara Y. FEMS Microbiol. Lett. 177:231-235(1999) [PubMed: 10474189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and expression of Mycobacterium tuberculosis and Mycobacterium leprae dihydropteroate synthase in Escherichia coli." Nopponpunth V., Sirawaraporn W., Greene P.J., Santi D.V. J. Bacteriol. 181:6814-6821(1999) [PubMed: 10542185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB028658 Genomic DNA. Translation: BAA84076.1. AB028659 Genomic DNA. Translation: BAA84077.1. AB028660 Genomic DNA. Translation: BAA84078.1. AB028661 Genomic DNA. Translation: BAA84079.1. AB028662 Genomic DNA. Translation: BAA84080.1. AB028663 Genomic DNA. Translation: BAA84081.1. AB028656 Genomic DNA. Translation: BAA84074.1. AB028657 Genomic DNA. Translation: BAA84075.1. AF117618 Genomic DNA. Translation: AAF06725.1. AL023093 Genomic DNA. Translation: CAA18794.1. AL583917 Genomic DNA. Translation: CAC29732.1.
PIR	H86936.
RefSeq	NP_301284.1. NC_002677.1.
3D structure databases
ProteinModelPortal	P0C0X1.
SMR	P0C0X1. Positions 5-273.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000028197; EBMYCP00000027803; EBMYCG00000028192.
GeneID	908646.
GenomeReviews	Gene locus ML0224 in contig AL450380_GR.
KEGG	mle:ML0224.
NMPDR	fig|272631.1.peg.156.
PATRIC	18050810. VBIMycLep78757_0356.
Organism-specific databases
Leproma	ML0224.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000015914.
HOGENOM	HBG754410.
OMA	VVMHWRG.
ProtClustDB	CLSK799350.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0224-MONOMER.
Family and domain databases
InterPro	IPR006390. DHP_synth. IPR011005. Dihydropteroate_synth-like. IPR000489. Pterin-binding. [Graphical view]
Gene3D	G3DSA:3.20.20.20. Dhdropt_synth. 1 hit.
KO	K00796.
Pfam	PF00809. Pterin_bind. 1 hit. [Graphical view]
SUPFAM	SSF51717. DHP_synth_like. 1 hit.
TIGRFAMs	TIGR01496. DHPS. 1 hit.
PROSITE	PS00792. DHPS_1. 1 hit. PS00793. DHPS_2. 1 hit. PS50972. PTERIN_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00250. Dapsone.

Entry information

Entry name

DHPS1_MYCLE

Accession

Primary (citable) accession number: P0C0X1
Secondary accession number(s): O69530 , P46812, Q9R2Q6, Q9R2U9, Q9S0T0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	January 10, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program