60S ribosomal protein L11-A - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P0C0W9 (RL11A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 74. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
60S ribosomal protein L11-A

Alternative name(s):
L16
RP39
YL22

Gene names

Name:	RPL11A
Synonyms:	RP39A, RPL16A
Ordered Locus Names:	YPR102C
ORF Names:	P8283.14

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to 5S ribosomal RNA.
Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Forms a heterotrimeric complex with RPL5 and SYO1. Interaction of this complex with KAP104 allows the nuclear import of the heterotrimer. Ref.7
Subcellular location	Cytoplasm. Nucleus. Note: The SYO1/RPL11/RPL5 complex is transported into the nucleus by KAP104. Ref.9 Ref.12
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Miscellaneous	Present with 39000 molecules/cell in log phase SD medium. There are 2 genes for L11 in yeast.
Sequence similarities	Belongs to the ribosomal protein L5P family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.7. Source: SGD ribosomal large subunit assembly Inferred from mutant phenotype PubMed 2277060. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.7. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Traceable author statement Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5 Ref.8

Chain

2 – 174

173

60S ribosomal protein L11-A

PRO_0000125104

Amino acid modifications

Modified residue

N-acetylserine Ref.5 Ref.8

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphothreonine Ref.11

Experimental info

Sequence conflict

L → D AA sequence Ref.5

Sequence conflict

E → Q in CAA25515. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P0C0W9 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 1E5C7D83541C6854
Show »

FASTA

174

19,720

        10         20         30         40         50         60 
MSAKAQNPMR DLKIEKLVLN ISVGESGDRL TRASKVLEQL SGQTPVQSKA RYTVRTFGIR 

        70         80         90        100        110        120 
RNEKIAVHVT VRGPKAEEIL ERGLKVKEYQ LRDRNFSATG NFGFGIDEHI DLGIKYDPSI 

       130        140        150        160        170 
GIFGMDFYVV MNRPGARVTR RKRCKGTVGN SHKTTKEDTV SWFKQKYDAD VLDK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of the gene encoding yeast ribosomal protein L16." Leer R.J., van Raamsdonk-Duin M.M.C., Mager W.H., Planta R.J. FEBS Lett. 175:371-376(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-17, ACETYLATION AT SER-2 BY NATA.
[6]	"Localization of Saccharomyces cerevisiae ribosomal protein L16 on the surface of 60 S ribosomal subunits by immunoelectron microscopy." Tsay Y.-F., Shankweiler G., Lake J., Woolford J.L. Jr. J. Biol. Chem. 269:7579-7586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[7]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[8]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22; SER-26 AND THR-31, MASS SPECTROMETRY.
[12]	"Synchronizing nuclear import of ribosomal proteins with ribosome assembly." Kressler D., Bange G., Ogawa Y., Stjepanovic G., Bradatsch B., Pratte D., Amlacher S., Strauss D., Yoneda Y., Katahira J., Sinning I., Hurt E. Science 338:666-671(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RPL5 AND SYO1, SUBCELLULAR LOCATION.
[13]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 7-171, ELECTRON MICROSCOPY.
[14]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X01029 Genomic DNA. Translation: CAA25515.1.
U32445 Genomic DNA. Translation: AAB68072.1.
AY693151 Genomic DNA. Translation: AAT93170.1.
BK006949 Genomic DNA. Translation: DAA11516.1.

PIR

S59767.

RefSeq

NP_015427.1. NM_001184199.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	J	7-171	[»]
1S1I	electron microscopy	11.70	J	2-174	[»]
3IZS	electron microscopy	-	E	1-174	[»]
3O58	X-ray	4.00	K	1-174	[»]
3O5H	X-ray	4.00	K	1-174	[»]
3U5E	X-ray	3.00	J	1-174	[»]
3U5I	X-ray	3.00	J	1-174	[»]

ProteinModelPortal

P0C0W9.

SMR

P0C0W9. Positions 6-174.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4764N.

IntAct

P0C0W9. 18 interactions.

MINT

MINT-8285559.

Proteomic databases

PRIDE

P0C0W9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YPR102C; YPR102C; YPR102C.

GeneID

856217.

KEGG

sce:YPR102C.

Organism-specific databases

SGD

S000006306. RPL11A.

Phylogenomic databases

GeneTree

ENSGT00390000013411.

HOGENOM

HOG000231312.

K02868.

OMA

HMGVGES.

OrthoDB

EOG4M0JB6.

Enzyme and pathway databases

BioCyc

YEAST:G3O-34242-MONOMER.

Gene expression databases

Genevestigator

P0C0W9.

Family and domain databases

Gene3D

3.30.1440.10. 1 hit.

InterPro

IPR002132. Ribosomal_L5.
IPR020929. Ribosomal_L5_CS.
IPR022803. Ribosomal_L5_domain.
[Graphical view]

PANTHER

PTHR11994. PTHR11994. 1 hit.

Pfam

PF00281. Ribosomal_L5. 1 hit.
PF00673. Ribosomal_L5_C. 1 hit.
[Graphical view]

PIRSF

PIRSF002161. Ribosomal_L5. 1 hit.

SUPFAM

SSF55282. Ribosomal_L5. 1 hit.

PROSITE

PS00358. RIBOSOMAL_L5. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0C0W9.

NextBio

981438.

Entry information

Entry name

RL11A_YEAST

Accession

Primary (citable) accession number: P0C0W9
Secondary accession number(s): D6W4A0, P06380

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents