P0C0W1 (RS22A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S22-A

Alternative name(s):
RP50
S24
YP58
YS22

Gene names

Name:	RPS22A
Synonyms:	RPS24, RPS24A
Ordered Locus Names:	YJL190C
ORF Names:	J0355

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	130 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm By similarity.
Miscellaneous	Present with 40400 molecules/cell in log phase SD medium. There are 2 genes for S22 in yeast.
Sequence similarities	Belongs to the ribosomal protein S8P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred by curator Ref.5. Source: SGD
Cellular_component	cytosolic small ribosomal subunit Inferred from direct assay Ref.5. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5 Ref.7

Chain

2 – 130

129

40S ribosomal protein S22-A

PRO_0000126623

Secondary structure

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130

Helix Strand Turn

Details...

Helix

6 – 19

14

Beta strand

23 – 27

5

Helix

32 – 42

11

Turn

43 – 45

3

Beta strand

50 – 53

4

Beta strand

56 – 58

3

Beta strand

60 – 64

5

Beta strand

72 – 74

3

Helix

85 – 93

9

Beta strand

94 – 96

3

Beta strand

97 – 99

3

Beta strand

101 – 106

6

Beta strand

109 – 112

4

Helix

113 – 119

7

Beta strand

123 – 129

7

Sequences

	Sequence		Length	Mass (Da)
	P0C0W1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: ED34BEF6A5442CC9	FASTA	130	14,626
	10 20 30 40 50 60 MTRSSVLADA LNAINNAEKT GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EYIDDHRSGK 70 80 90 100 110 120 IVVQLNGRLN KCGVISPRFN VKIGDIEKWT ANLLPARQFG YVILTTSAGI MDHEEARRKH 130 VSGKILGFVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genes for yeast ribosomal proteins S24 and L46 are adjacent and divergently transcribed." Leer R.J., van Raamsdonk-Duin M.M.C., Kraakman P., Mager W.H., Planta R.J. Nucleic Acids Res. 13:701-709(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes." Purnelle B., Coster F., Goffeau A. Yeast 10:1235-1249(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast." Otaka E., Higo K., Osawa S. Biochemistry 21:4545-4550(1982) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-44.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 4-130, ELECTRON MICROSCOPY.
[10]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X01962 Genomic DNA. Translation: CAA25998.1.
X77688 Genomic DNA. Translation: CAA54770.1.
Z49465 Genomic DNA. Translation: CAA89485.1.
BK006943 Genomic DNA. Translation: DAA08617.1.

PIR

R4BY24. A23082.

RefSeq

NP_012345.1. NM_001181623.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5X	model	-	H	4-130	[»]
1S1H	electron microscopy	11.70	H	2-130	[»]
3IZB	electron microscopy	-	H	1-130	[»]
3O2Z	X-ray	4.00	O	1-130	[»]
3O30	X-ray	4.00	O	1-130	[»]
3U5C	X-ray	3.00	W	1-130	[»]
3U5G	X-ray	3.00	W	1-130	[»]

ProteinModelPortal

P0C0W1.

SMR

P0C0W1. Positions 2-130.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5473N.

IntAct

P0C0W1. 34 interactions.

MINT

MINT-479211.

STRING

4932.YJL190C.

Proteomic databases

PeptideAtlas

P0C0W1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJL190C; YJL190C; YJL190C.

GeneID

853249.

KEGG

sce:YJL190C.

Organism-specific databases

SGD

S000003726. RPS22A.

Phylogenomic databases

GeneTree

ENSGT00390000003021.

HOGENOM

HOG000204097.

KO

K02957.

OMA

FEKWEKR.

OrthoDB

EOG4S4SRR.

Enzyme and pathway databases

BioCyc

YEAST:G3O-31622-MONOMER.

Gene expression databases

Genevestigator

P0C0W1.

Family and domain databases

InterPro

IPR000630. Ribosomal_S8.
[Graphical view]

PANTHER

PTHR11758. PTHR11758. 1 hit.

Pfam

PF00410. Ribosomal_S8. 1 hit.
[Graphical view]

SUPFAM

SSF56047. Ribosomal_S8. 1 hit.

PROSITE

PS00053. RIBOSOMAL_S8. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0C0W1.

NextBio

973490.

Entry information

Entry name

RS22A_YEAST

Accession

Primary (citable) accession number: P0C0W1
Secondary accession number(s): D6VW01, P04648

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program