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P0C0V9

- HEMA_BRV1

UniProt

P0C0V9 - HEMA_BRV1

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Protein
Hemagglutinin-esterase
Gene
HE
Organism
Breda virus 1 (BRV-1)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-di-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. Also hydrolyzes 5-N-acetyl-4-O-acetylneuraminic acid and N-acetyl-9-O-acetylneuraminic acid, but displays a substrate preference for N-acetyl-9-di-O-acetylneuraminic acid. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei37 – 371Nucleophile By similarity
Active sitei223 – 2231Charge relay system By similarity
Active sitei328 – 3281Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
OrganismiBreda virus 1 (BRV-1)
Taxonomic identifieri360393 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeTorovirinaeTorovirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000000355: Genome

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host cell membrane; Single-pass type I membrane protein Reviewed prediction
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini15 – 393379Virion surface Reviewed prediction
Add
BLAST
Transmembranei394 – 41421Helical; Reviewed prediction
Add
BLAST
Topological domaini415 – 4162Intravirion Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1414 Reviewed prediction
Add
BLAST
Chaini15 – 416402Hemagglutinin-esterase
PRO_0000045398Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi76 – 761N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi257 – 2571N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi278 – 2781N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi313 – 3131N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi322 – 3221N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi343 – 3431N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

N-glycosylated By similarity.

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

DIPiDIP-48962N.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi21 – 233
Beta strandi27 – 293
Beta strandi31 – 366
Helixi37 – 393
Turni44 – 496
Helixi54 – 596
Beta strandi60 – 623
Helixi68 – 736
Beta strandi86 – 883
Beta strandi90 – 956
Helixi102 – 1043
Beta strandi108 – 1114
Helixi112 – 13120
Beta strandi135 – 1417
Beta strandi151 – 1533
Beta strandi164 – 17310
Beta strandi177 – 1837
Beta strandi185 – 1873
Beta strandi193 – 20210
Beta strandi207 – 2093
Beta strandi212 – 2143
Beta strandi216 – 2227
Beta strandi225 – 2317
Beta strandi244 – 2518
Beta strandi253 – 26715
Beta strandi269 – 2746
Beta strandi283 – 2864
Helixi299 – 3035
Turni306 – 3083
Beta strandi309 – 3124
Beta strandi320 – 3256
Beta strandi328 – 3303
Helixi331 – 3377
Helixi338 – 3414
Beta strandi345 – 3484
Beta strandi351 – 3533
Beta strandi357 – 3615
Beta strandi368 – 3703
Helixi375 – 3784

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3I26X-ray1.80A/B/C/D15-392[»]
3I27X-ray2.00A/B/C/D15-392[»]
ProteinModelPortaliP0C0V9.

Miscellaneous databases

EvolutionaryTraceiP0C0V9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 121118Esterase domain first part By similarity
Add
BLAST
Regioni122 – 263142Receptor binding By similarity
Add
BLAST
Regioni264 – 379116Esterase domain second part By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi61 – 677Poly-Ser
Compositional biasi402 – 4054Poly-Val

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C0V9-1 [UniParc]FASTAAdd to Basket

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MLSLILFFPS FAFAATPVTP YYGPGHITFD WCGFGDSRSD CTNPQSPMSL    50
DIPQQLCPKF SSKSSSSMFL SLHWNNHSSF VSYDYFNCGV EKVFYEGVNF 100
SPRKQYSCWD EGVDGWIELK TRFYTKLYQM ATTSRCIKLI QLQAPSSLPT 150
LQAGVCRTNK QLPDNPRLAL LSDTVPTSVQ FVLPGSSGTT ICTKHLVPFC 200
YLNHGCFTTG GSCLPFGVSY VSDSFYYGYY DATPQIGSTE SHDYVCDYLF 250
MEPGTYNAST VGKFLVYPTK SYCMDTMNIT VPVQAVQSIW SEQYASDDAI 300
GQACKAPYCI FYNKTTPYTV TNGSDANHGD DEVRMMMQGL LRNSSCISPQ 350
GSTPLALYST EMIYEPNYGS CPQFYKLFDT SGNENIDVIS SSYFVATWVL 400
LVVVVILIFV IISFFC 416
Length:416
Mass (Da):46,440
Last modified:January 10, 2006 - v1
Checksum:i384C406385439054
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 291F → S.
Natural varianti114 – 1141D → G.
Natural varianti133 – 1331T → I.
Natural varianti152 – 1521Q → K.
Natural varianti155 – 1551V → E.
Natural varianti159 – 1591N → K.
Natural varianti214 – 2141L → R.
Natural varianti234 – 2341P → S.
Natural varianti238 – 2381S → L.
Natural varianti416 – 4161C → V.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF076621 Genomic RNA. Translation: AAD03842.1.
AY427798 Genomic RNA. Translation: AAS17961.1.
RefSeqiYP_337909.1. NC_007447.1.

Genome annotation databases

GeneIDi3707767.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF076621 Genomic RNA. Translation: AAD03842.1 .
AY427798 Genomic RNA. Translation: AAS17961.1 .
RefSeqi YP_337909.1. NC_007447.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3I26 X-ray 1.80 A/B/C/D 15-392 [» ]
3I27 X-ray 2.00 A/B/C/D 15-392 [» ]
ProteinModelPortali P0C0V9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48962N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3707767.

Miscellaneous databases

EvolutionaryTracei P0C0V9.

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Bovine torovirus: sequencing of the structural genes and expression of the nucleocapsid protein of Breda virus."
    Duckmanton L.M., Tellier R., Liu P., Petric M.
    Virus Res. 58:83-96(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "The complete sequence of the bovine torovirus genome."
    Draker R., Roper R.L., Petric M., Tellier R.
    Virus Res. 115:56-68(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Nidovirus sialate-O-acetylesterases: evolution and substrate specificity of coronaviral and toroviral receptor-destroying enzymes."
    Smits S.L., Gerwig G.J., van Vliet A.L., Lissenberg A., Briza P., Kamerling J.P., Vlasak R., de Groot R.J.
    J. Biol. Chem. 280:6933-6941(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHEMA_BRV1
AccessioniPrimary (citable) accession number: P0C0V9
Secondary accession number(s): O39517, Q3T8I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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