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Protein

40S ribosomal protein S21-A

Gene

RPS21A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed:22096102). eS21 is required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (PubMed:14627813).1 Publication1 Publication

Miscellaneous

Present with 27400 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eS21 in yeast.Curated

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein
Biological processrRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-32026-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S21-A1 Publication
Alternative name(s):
S26
Small ribosomal subunit protein eS21-A1 Publication
YS25
Gene namesi
Name:RPS21A1 Publication
Synonyms:RPS25, RPS25A, RPS26A
Ordered Locus Names:YKR057W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR057W.
SGDiS000001765. RPS21A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001947611 – 8740S ribosomal protein S21-AAdd BLAST87

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatB.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP0C0V8.
PRIDEiP0C0V8.
TopDownProteomicsiP0C0V8.

2D gel databases

UCD-2DPAGEiP0C0V8.

PTM databases

iPTMnetiP0C0V8.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi34188. 210 interactors.
IntActiP0C0V8. 8 interactors.
MINTiMINT-4493667.

Structurei

Secondary structure

187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Turni18 – 20Combined sources3
Beta strandi32 – 37Combined sources6
Beta strandi41 – 43Combined sources3
Beta strandi50 – 55Combined sources6
Helixi57 – 61Combined sources5
Turni62 – 64Combined sources3
Helixi65 – 74Combined sources10
Turni75 – 77Combined sources3
Beta strandi80 – 82Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10211-87[»]
3J6Yelectron microscopy6.10211-87[»]
3J77electron microscopy6.20211-87[»]
3J78electron microscopy6.30211-87[»]
3V88X-ray3.00V1-87[»]
4U3MX-ray3.00D1/d11-87[»]
4U3NX-ray3.20D1/d11-87[»]
4U3UX-ray2.90D1/d11-87[»]
4U4NX-ray3.10D1/d11-87[»]
4U4OX-ray3.60D1/d11-87[»]
4U4QX-ray3.00D1/d11-87[»]
4U4RX-ray2.80D1/d11-87[»]
4U4UX-ray3.00D1/d11-87[»]
4U4YX-ray3.20D1/d11-87[»]
4U4ZX-ray3.10D1/d11-87[»]
4U50X-ray3.20D1/d11-87[»]
4U51X-ray3.20D1/d11-87[»]
4U52X-ray3.00D1/d11-87[»]
4U53X-ray3.30D1/d11-87[»]
4U55X-ray3.20D1/d11-87[»]
4U56X-ray3.45D1/d11-87[»]
4U6FX-ray3.10D1/d11-87[»]
4V6Ielectron microscopy8.80AT1-87[»]
4V88X-ray3.00AV/CV1-87[»]
4V8Yelectron microscopy4.30AV1-87[»]
4V8Zelectron microscopy6.60AV1-87[»]
4V92electron microscopy3.70V1-87[»]
5DATX-ray3.15D1/d11-87[»]
5DC3X-ray3.25D1/d11-87[»]
5DGEX-ray3.45D1/d11-87[»]
5DGFX-ray3.30D1/d11-87[»]
5DGVX-ray3.10D1/d11-87[»]
5FCIX-ray3.40D1/d11-87[»]
5FCJX-ray3.10D1/d11-87[»]
5I4LX-ray3.10D1/d11-87[»]
5JUOelectron microscopy4.00SB1-87[»]
5JUPelectron microscopy3.50SB1-87[»]
5JUSelectron microscopy4.20SB1-87[»]
5JUTelectron microscopy4.00SB1-87[»]
5JUUelectron microscopy4.00SB1-87[»]
5LYBX-ray3.25D1/d11-87[»]
5M1Jelectron microscopy3.30V21-87[»]
5MC6electron microscopy3.80a1-87[»]
5TGAX-ray3.30D1/d11-87[»]
5TGMX-ray3.50D1/d11-87[»]
ProteinModelPortaliP0C0V8.
SMRiP0C0V8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000017515.
HOGENOMiHOG000183557.
InParanoidiP0C0V8.
KOiK02971.
OMAiTRYAICG.
OrthoDBiEOG092C5XGV.

Family and domain databases

InterProiView protein in InterPro
IPR001931. Ribosomal_S21e.
IPR018279. Ribosomal_S21e_CS.
PANTHERiPTHR10442. PTHR10442. 1 hit.
PfamiView protein in Pfam
PF01249. Ribosomal_S21e. 1 hit.
PIRSFiPIRSF002148. Ribosomal_S21e. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD006584. Ribosomal_S21e. 1 hit.
PROSITEiView protein in PROSITE
PS00996. RIBOSOMAL_S21E. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C0V8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY
60 70 80
VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR
Length:87
Mass (Da):9,746
Last modified:December 20, 2005 - v1
Checksum:i52E1C24744E24F80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07811 Genomic DNA. Translation: CAA30671.1.
Z28282 Genomic DNA. Translation: CAA82135.1.
Z28283 Genomic DNA. Translation: CAA82137.1.
BK006944 Genomic DNA. Translation: DAA09208.1.
PIRiS00975. R3BY1E.
RefSeqiNP_012983.3. NM_001179847.3.

Genome annotation databases

EnsemblFungiiYKR057W; YKR057W; YKR057W.
GeneIDi853931.
KEGGisce:YKR057W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07811 Genomic DNA. Translation: CAA30671.1.
Z28282 Genomic DNA. Translation: CAA82135.1.
Z28283 Genomic DNA. Translation: CAA82137.1.
BK006944 Genomic DNA. Translation: DAA09208.1.
PIRiS00975. R3BY1E.
RefSeqiNP_012983.3. NM_001179847.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10211-87[»]
3J6Yelectron microscopy6.10211-87[»]
3J77electron microscopy6.20211-87[»]
3J78electron microscopy6.30211-87[»]
3V88X-ray3.00V1-87[»]
4U3MX-ray3.00D1/d11-87[»]
4U3NX-ray3.20D1/d11-87[»]
4U3UX-ray2.90D1/d11-87[»]
4U4NX-ray3.10D1/d11-87[»]
4U4OX-ray3.60D1/d11-87[»]
4U4QX-ray3.00D1/d11-87[»]
4U4RX-ray2.80D1/d11-87[»]
4U4UX-ray3.00D1/d11-87[»]
4U4YX-ray3.20D1/d11-87[»]
4U4ZX-ray3.10D1/d11-87[»]
4U50X-ray3.20D1/d11-87[»]
4U51X-ray3.20D1/d11-87[»]
4U52X-ray3.00D1/d11-87[»]
4U53X-ray3.30D1/d11-87[»]
4U55X-ray3.20D1/d11-87[»]
4U56X-ray3.45D1/d11-87[»]
4U6FX-ray3.10D1/d11-87[»]
4V6Ielectron microscopy8.80AT1-87[»]
4V88X-ray3.00AV/CV1-87[»]
4V8Yelectron microscopy4.30AV1-87[»]
4V8Zelectron microscopy6.60AV1-87[»]
4V92electron microscopy3.70V1-87[»]
5DATX-ray3.15D1/d11-87[»]
5DC3X-ray3.25D1/d11-87[»]
5DGEX-ray3.45D1/d11-87[»]
5DGFX-ray3.30D1/d11-87[»]
5DGVX-ray3.10D1/d11-87[»]
5FCIX-ray3.40D1/d11-87[»]
5FCJX-ray3.10D1/d11-87[»]
5I4LX-ray3.10D1/d11-87[»]
5JUOelectron microscopy4.00SB1-87[»]
5JUPelectron microscopy3.50SB1-87[»]
5JUSelectron microscopy4.20SB1-87[»]
5JUTelectron microscopy4.00SB1-87[»]
5JUUelectron microscopy4.00SB1-87[»]
5LYBX-ray3.25D1/d11-87[»]
5M1Jelectron microscopy3.30V21-87[»]
5MC6electron microscopy3.80a1-87[»]
5TGAX-ray3.30D1/d11-87[»]
5TGMX-ray3.50D1/d11-87[»]
ProteinModelPortaliP0C0V8.
SMRiP0C0V8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34188. 210 interactors.
IntActiP0C0V8. 8 interactors.
MINTiMINT-4493667.

PTM databases

iPTMnetiP0C0V8.

2D gel databases

UCD-2DPAGEiP0C0V8.

Proteomic databases

MaxQBiP0C0V8.
PRIDEiP0C0V8.
TopDownProteomicsiP0C0V8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR057W; YKR057W; YKR057W.
GeneIDi853931.
KEGGisce:YKR057W.

Organism-specific databases

EuPathDBiFungiDB:YKR057W.
SGDiS000001765. RPS21A.

Phylogenomic databases

GeneTreeiENSGT00390000017515.
HOGENOMiHOG000183557.
InParanoidiP0C0V8.
KOiK02971.
OMAiTRYAICG.
OrthoDBiEOG092C5XGV.

Enzyme and pathway databases

BioCyciYEAST:G3O-32026-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiPR:P0C0V8.

Family and domain databases

InterProiView protein in InterPro
IPR001931. Ribosomal_S21e.
IPR018279. Ribosomal_S21e_CS.
PANTHERiPTHR10442. PTHR10442. 1 hit.
PfamiView protein in Pfam
PF01249. Ribosomal_S21e. 1 hit.
PIRSFiPIRSF002148. Ribosomal_S21e. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD006584. Ribosomal_S21e. 1 hit.
PROSITEiView protein in PROSITE
PS00996. RIBOSOMAL_S21E. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRS21A_YEAST
AccessioniPrimary (citable) accession number: P0C0V8
Secondary accession number(s): D6VXB8, P05760
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: April 12, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.