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Protein

40S ribosomal protein S21-A

Gene

RPS21A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed:22096102). eS21 is required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (PubMed:14627813).1 Publication1 Publication

Miscellaneous

Present with 27400 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eS21 in yeast.Curated

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein
Biological processrRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-32026-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-SCE-72702 Ribosomal scanning and start codon recognition
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S21-A1 Publication
Alternative name(s):
S26
Small ribosomal subunit protein eS21-A1 Publication
YS25
Gene namesi
Name:RPS21A1 Publication
Synonyms:RPS25, RPS25A, RPS26A
Ordered Locus Names:YKR057W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR057W
SGDiS000001765 RPS21A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001947611 – 8740S ribosomal protein S21-AAdd BLAST87

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatB.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP0C0V8
PaxDbiP0C0V8
PRIDEiP0C0V8
TopDownProteomicsiP0C0V8

2D gel databases

UCD-2DPAGEP0C0V8

PTM databases

iPTMnetiP0C0V8

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi34188, 218 interactors
IntActiP0C0V8, 8 interactors
MINTiP0C0V8
STRINGi4932.YKR057W

Structurei

Secondary structure

187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni18 – 20Combined sources3
Beta strandi32 – 39Combined sources8
Beta strandi45 – 55Combined sources11
Helixi57 – 61Combined sources5
Helixi65 – 75Combined sources11
Beta strandi80 – 82Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10211-87[»]
3J6Yelectron microscopy6.10211-87[»]
3J77electron microscopy6.20211-87[»]
3J78electron microscopy6.30211-87[»]
4U3MX-ray3.00D1/d11-87[»]
4U3NX-ray3.20D1/d11-87[»]
4U3UX-ray2.90D1/d11-87[»]
4U4NX-ray3.10D1/d11-87[»]
4U4OX-ray3.60D1/d11-87[»]
4U4QX-ray3.00D1/d11-87[»]
4U4RX-ray2.80D1/d11-87[»]
4U4UX-ray3.00D1/d11-87[»]
4U4YX-ray3.20D1/d11-87[»]
4U4ZX-ray3.10D1/d11-87[»]
4U50X-ray3.20D1/d11-87[»]
4U51X-ray3.20D1/d11-87[»]
4U52X-ray3.00D1/d11-87[»]
4U53X-ray3.30D1/d11-87[»]
4U55X-ray3.20D1/d11-87[»]
4U56X-ray3.45D1/d11-87[»]
4U6FX-ray3.10D1/d11-87[»]
4V6Ielectron microscopy8.80AT1-87[»]
4V88X-ray3.00AV/CV1-87[»]
4V8Yelectron microscopy4.30AV1-87[»]
4V8Zelectron microscopy6.60AV1-87[»]
4V92electron microscopy3.70V1-87[»]
5DATX-ray3.15D1/d11-87[»]
5DC3X-ray3.25D1/d11-87[»]
5DGEX-ray3.45D1/d11-87[»]
5DGFX-ray3.30D1/d11-87[»]
5DGVX-ray3.10D1/d11-87[»]
5FCIX-ray3.40D1/d11-87[»]
5FCJX-ray3.10D1/d11-87[»]
5I4LX-ray3.10D1/d11-87[»]
5JUOelectron microscopy4.00SB1-87[»]
5JUPelectron microscopy3.50SB1-87[»]
5JUSelectron microscopy4.20SB1-87[»]
5JUTelectron microscopy4.00SB1-87[»]
5JUUelectron microscopy4.00SB1-87[»]
5LL6electron microscopy3.90a1-87[»]
5LYBX-ray3.25D1/d11-87[»]
5M1Jelectron microscopy3.30V21-87[»]
5MC6electron microscopy3.80a1-87[»]
5MEIX-ray3.50W/d11-87[»]
5NDGX-ray3.70D1/d11-87[»]
5NDVX-ray3.30D1/d11-87[»]
5NDWX-ray3.70D1/d11-87[»]
5OBMX-ray3.40D1/d11-87[»]
5ON6X-ray3.10W/d11-87[»]
5TBWX-ray3.00W/d11-87[»]
5TGAX-ray3.30D1/d11-87[»]
5TGMX-ray3.50D1/d11-87[»]
6EMLelectron microscopy3.60a1-87[»]
6FAIelectron microscopy3.40V1-87[»]
ProteinModelPortaliP0C0V8
SMRiP0C0V8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000017515
HOGENOMiHOG000183557
InParanoidiP0C0V8
KOiK02971
OMAiIELYIPR
OrthoDBiEOG092C5XGV

Family and domain databases

Gene3Di3.30.1230.20, 1 hit
InterProiView protein in InterPro
IPR001931 Ribosomal_S21e
IPR018279 Ribosomal_S21e_CS
IPR038579 Ribosomal_S21e_sf
PANTHERiPTHR10442 PTHR10442, 1 hit
PfamiView protein in Pfam
PF01249 Ribosomal_S21e, 1 hit
PIRSFiPIRSF002148 Ribosomal_S21e, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD006584 Ribosomal_S21e, 1 hit
PROSITEiView protein in PROSITE
PS00996 RIBOSOMAL_S21E, 1 hit

Sequencei

Sequence statusi: Complete.

P0C0V8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY
60 70 80
VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR
Length:87
Mass (Da):9,746
Last modified:December 20, 2005 - v1
Checksum:i52E1C24744E24F80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07811 Genomic DNA Translation: CAA30671.1
Z28282 Genomic DNA Translation: CAA82135.1
Z28283 Genomic DNA Translation: CAA82137.1
BK006944 Genomic DNA Translation: DAA09208.1
PIRiS00975 R3BY1E
RefSeqiNP_012983.3, NM_001179847.3

Genome annotation databases

EnsemblFungiiYKR057W; YKR057W; YKR057W
GeneIDi853931
KEGGisce:YKR057W

Similar proteinsi

Entry informationi

Entry nameiRS21A_YEAST
AccessioniPrimary (citable) accession number: P0C0V8
Secondary accession number(s): D6VXB8, P05760
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 23, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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