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Protein

40S ribosomal protein S21-A

Gene

RPS21A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability.1 Publication

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-32026-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S21-A
Alternative name(s):
S26
YS25
Gene namesi
Name:RPS21A
Synonyms:RPS25, RPS25A, RPS26A
Ordered Locus Names:YKR057W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR057W.
SGDiS000001765. RPS21A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 878740S ribosomal protein S21-APRO_0000194761Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatB.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

TopDownProteomicsiP0C0V8.

2D gel databases

UCD-2DPAGEP0C0V8.

PTM databases

iPTMnetiP0C0V8.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi34188. 59 interactions.
IntActiP0C0V8. 8 interactions.
MINTiMINT-4493667.

Structurei

Secondary structure

1
87
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 63Combined sources
Turni18 – 203Combined sources
Beta strandi32 – 376Combined sources
Beta strandi41 – 433Combined sources
Beta strandi50 – 556Combined sources
Helixi57 – 615Combined sources
Turni62 – 643Combined sources
Helixi65 – 7410Combined sources
Turni75 – 773Combined sources
Beta strandi80 – 823Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10211-87[»]
3J6Yelectron microscopy6.10211-87[»]
3J77electron microscopy6.20211-87[»]
3J78electron microscopy6.30211-87[»]
3V88X-ray3.00V1-87[»]
4U3MX-ray3.00D1/d11-87[»]
4U3NX-ray3.20D1/d11-87[»]
4U3UX-ray2.90D1/d11-87[»]
4U4NX-ray3.10D1/d11-87[»]
4U4OX-ray3.60D1/d11-87[»]
4U4QX-ray3.00D1/d11-87[»]
4U4RX-ray2.80D1/d11-87[»]
4U4UX-ray3.00D1/d11-87[»]
4U4YX-ray3.20D1/d11-87[»]
4U4ZX-ray3.10D1/d11-87[»]
4U50X-ray3.20D1/d11-87[»]
4U51X-ray3.20D1/d11-87[»]
4U52X-ray3.00D1/d11-87[»]
4U53X-ray3.30D1/d11-87[»]
4U55X-ray3.20D1/d11-87[»]
4U56X-ray3.45D1/d11-87[»]
4U6FX-ray3.10D1/d11-87[»]
4V6Ielectron microscopy8.80AT1-87[»]
4V88X-ray3.00AV/CV1-87[»]
4V8Yelectron microscopy4.30AV1-87[»]
4V8Zelectron microscopy6.60AV1-87[»]
4V92electron microscopy3.70V1-87[»]
5FCIX-ray3.40D1/d11-87[»]
5FCJX-ray3.10D1/d11-87[»]
ProteinModelPortaliP0C0V8.
SMRiP0C0V8. Positions 1-87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S21e family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000017515.
HOGENOMiHOG000183557.
InParanoidiP0C0V8.
KOiK02971.
OMAiTRYAICG.
OrthoDBiEOG7FNCM4.

Family and domain databases

InterProiIPR001931. Ribosomal_S21e.
IPR018279. Ribosomal_S21e_CS.
[Graphical view]
PANTHERiPTHR10442. PTHR10442. 1 hit.
PfamiPF01249. Ribosomal_S21e. 1 hit.
[Graphical view]
PIRSFiPIRSF002148. Ribosomal_S21e. 1 hit.
ProDomiPD006584. Ribosomal_S21e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00996. RIBOSOMAL_S21E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C0V8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY
60 70 80
VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR
Length:87
Mass (Da):9,746
Last modified:December 20, 2005 - v1
Checksum:i52E1C24744E24F80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07811 Genomic DNA. Translation: CAA30671.1.
Z28282 Genomic DNA. Translation: CAA82135.1.
Z28283 Genomic DNA. Translation: CAA82137.1.
BK006944 Genomic DNA. Translation: DAA09208.1.
PIRiS00975. R3BY1E.
RefSeqiNP_012983.3. NM_001179847.3.

Genome annotation databases

EnsemblFungiiYKR057W; YKR057W; YKR057W.
GeneIDi853931.
KEGGisce:YKR057W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07811 Genomic DNA. Translation: CAA30671.1.
Z28282 Genomic DNA. Translation: CAA82135.1.
Z28283 Genomic DNA. Translation: CAA82137.1.
BK006944 Genomic DNA. Translation: DAA09208.1.
PIRiS00975. R3BY1E.
RefSeqiNP_012983.3. NM_001179847.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10211-87[»]
3J6Yelectron microscopy6.10211-87[»]
3J77electron microscopy6.20211-87[»]
3J78electron microscopy6.30211-87[»]
3V88X-ray3.00V1-87[»]
4U3MX-ray3.00D1/d11-87[»]
4U3NX-ray3.20D1/d11-87[»]
4U3UX-ray2.90D1/d11-87[»]
4U4NX-ray3.10D1/d11-87[»]
4U4OX-ray3.60D1/d11-87[»]
4U4QX-ray3.00D1/d11-87[»]
4U4RX-ray2.80D1/d11-87[»]
4U4UX-ray3.00D1/d11-87[»]
4U4YX-ray3.20D1/d11-87[»]
4U4ZX-ray3.10D1/d11-87[»]
4U50X-ray3.20D1/d11-87[»]
4U51X-ray3.20D1/d11-87[»]
4U52X-ray3.00D1/d11-87[»]
4U53X-ray3.30D1/d11-87[»]
4U55X-ray3.20D1/d11-87[»]
4U56X-ray3.45D1/d11-87[»]
4U6FX-ray3.10D1/d11-87[»]
4V6Ielectron microscopy8.80AT1-87[»]
4V88X-ray3.00AV/CV1-87[»]
4V8Yelectron microscopy4.30AV1-87[»]
4V8Zelectron microscopy6.60AV1-87[»]
4V92electron microscopy3.70V1-87[»]
5FCIX-ray3.40D1/d11-87[»]
5FCJX-ray3.10D1/d11-87[»]
ProteinModelPortaliP0C0V8.
SMRiP0C0V8. Positions 1-87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34188. 59 interactions.
IntActiP0C0V8. 8 interactions.
MINTiMINT-4493667.

PTM databases

iPTMnetiP0C0V8.

2D gel databases

UCD-2DPAGEP0C0V8.

Proteomic databases

TopDownProteomicsiP0C0V8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR057W; YKR057W; YKR057W.
GeneIDi853931.
KEGGisce:YKR057W.

Organism-specific databases

EuPathDBiFungiDB:YKR057W.
SGDiS000001765. RPS21A.

Phylogenomic databases

GeneTreeiENSGT00390000017515.
HOGENOMiHOG000183557.
InParanoidiP0C0V8.
KOiK02971.
OMAiTRYAICG.
OrthoDBiEOG7FNCM4.

Enzyme and pathway databases

BioCyciYEAST:G3O-32026-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiP0C0V8.

Family and domain databases

InterProiIPR001931. Ribosomal_S21e.
IPR018279. Ribosomal_S21e_CS.
[Graphical view]
PANTHERiPTHR10442. PTHR10442. 1 hit.
PfamiPF01249. Ribosomal_S21e. 1 hit.
[Graphical view]
PIRSFiPIRSF002148. Ribosomal_S21e. 1 hit.
ProDomiPD006584. Ribosomal_S21e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00996. RIBOSOMAL_S21E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structures of ribosomal protein YS25 from Saccharomyces cerevisiae and its counterparts from Schizosaccharomyces pombe and rat liver."
    Itoh T., Otaka E., Matsui K.A.
    Biochemistry 24:7418-7423(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  2. "Cloning and nucleotide sequence of the gene encoding yeast ribosomal protein YS25."
    Suzuki K., Otaka E.
    Nucleic Acids Res. 16:6223-6223(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  6. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT MET-1 BY NATB.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA."
    Tabb-Massey A., Caffrey J.M., Logsden P., Taylor S., Trent J.O., Ellis S.R.
    Nucleic Acids Res. 31:6798-6805(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiRS21A_YEAST
AccessioniPrimary (citable) accession number: P0C0V8
Secondary accession number(s): D6VXB8, P05760
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: July 6, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 27400 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S21 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.