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Protein

40S ribosomal protein S21-A

Gene

RPS21A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability.1 Publication

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-32026-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S21-A
Alternative name(s):
S26
YS25
Gene namesi
Name:RPS21A
Synonyms:RPS25, RPS25A, RPS26A
Ordered Locus Names:YKR057W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR057W.
SGDiS000001765. RPS21A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001947611 – 8740S ribosomal protein S21-AAdd BLAST87

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatB.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP0C0V8.
PRIDEiP0C0V8.
TopDownProteomicsiP0C0V8.

2D gel databases

UCD-2DPAGEP0C0V8.

PTM databases

iPTMnetiP0C0V8.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi34188. 59 interactors.
IntActiP0C0V8. 8 interactors.
MINTiMINT-4493667.

Structurei

Secondary structure

187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Turni18 – 20Combined sources3
Beta strandi32 – 37Combined sources6
Beta strandi41 – 43Combined sources3
Beta strandi50 – 55Combined sources6
Helixi57 – 61Combined sources5
Turni62 – 64Combined sources3
Helixi65 – 74Combined sources10
Turni75 – 77Combined sources3
Beta strandi80 – 82Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10211-87[»]
3J6Yelectron microscopy6.10211-87[»]
3J77electron microscopy6.20211-87[»]
3J78electron microscopy6.30211-87[»]
3V88X-ray3.00V1-87[»]
4U3MX-ray3.00D1/d11-87[»]
4U3NX-ray3.20D1/d11-87[»]
4U3UX-ray2.90D1/d11-87[»]
4U4NX-ray3.10D1/d11-87[»]
4U4OX-ray3.60D1/d11-87[»]
4U4QX-ray3.00D1/d11-87[»]
4U4RX-ray2.80D1/d11-87[»]
4U4UX-ray3.00D1/d11-87[»]
4U4YX-ray3.20D1/d11-87[»]
4U4ZX-ray3.10D1/d11-87[»]
4U50X-ray3.20D1/d11-87[»]
4U51X-ray3.20D1/d11-87[»]
4U52X-ray3.00D1/d11-87[»]
4U53X-ray3.30D1/d11-87[»]
4U55X-ray3.20D1/d11-87[»]
4U56X-ray3.45D1/d11-87[»]
4U6FX-ray3.10D1/d11-87[»]
4V6Ielectron microscopy8.80AT1-87[»]
4V88X-ray3.00AV/CV1-87[»]
4V8Yelectron microscopy4.30AV1-87[»]
4V8Zelectron microscopy6.60AV1-87[»]
4V92electron microscopy3.70V1-87[»]
5DATX-ray3.15D1/d11-87[»]
5DC3X-ray3.25D1/d11-87[»]
5FCIX-ray3.40D1/d11-87[»]
5FCJX-ray3.10D1/d11-87[»]
5I4LX-ray3.10D1/d11-87[»]
5JUOelectron microscopy4.00SB1-87[»]
5JUPelectron microscopy3.50SB1-87[»]
5JUSelectron microscopy4.20SB1-87[»]
5JUTelectron microscopy4.00SB1-87[»]
5JUUelectron microscopy4.00SB1-87[»]
ProteinModelPortaliP0C0V8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S21e family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000017515.
HOGENOMiHOG000183557.
InParanoidiP0C0V8.
KOiK02971.
OMAiTRYAICG.
OrthoDBiEOG092C5XGV.

Family and domain databases

InterProiIPR001931. Ribosomal_S21e.
IPR018279. Ribosomal_S21e_CS.
[Graphical view]
PANTHERiPTHR10442. PTHR10442. 1 hit.
PfamiPF01249. Ribosomal_S21e. 1 hit.
[Graphical view]
PIRSFiPIRSF002148. Ribosomal_S21e. 1 hit.
ProDomiPD006584. Ribosomal_S21e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00996. RIBOSOMAL_S21E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C0V8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY
60 70 80
VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR
Length:87
Mass (Da):9,746
Last modified:December 20, 2005 - v1
Checksum:i52E1C24744E24F80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07811 Genomic DNA. Translation: CAA30671.1.
Z28282 Genomic DNA. Translation: CAA82135.1.
Z28283 Genomic DNA. Translation: CAA82137.1.
BK006944 Genomic DNA. Translation: DAA09208.1.
PIRiS00975. R3BY1E.
RefSeqiNP_012983.3. NM_001179847.3.

Genome annotation databases

EnsemblFungiiYKR057W; YKR057W; YKR057W.
GeneIDi853931.
KEGGisce:YKR057W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07811 Genomic DNA. Translation: CAA30671.1.
Z28282 Genomic DNA. Translation: CAA82135.1.
Z28283 Genomic DNA. Translation: CAA82137.1.
BK006944 Genomic DNA. Translation: DAA09208.1.
PIRiS00975. R3BY1E.
RefSeqiNP_012983.3. NM_001179847.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10211-87[»]
3J6Yelectron microscopy6.10211-87[»]
3J77electron microscopy6.20211-87[»]
3J78electron microscopy6.30211-87[»]
3V88X-ray3.00V1-87[»]
4U3MX-ray3.00D1/d11-87[»]
4U3NX-ray3.20D1/d11-87[»]
4U3UX-ray2.90D1/d11-87[»]
4U4NX-ray3.10D1/d11-87[»]
4U4OX-ray3.60D1/d11-87[»]
4U4QX-ray3.00D1/d11-87[»]
4U4RX-ray2.80D1/d11-87[»]
4U4UX-ray3.00D1/d11-87[»]
4U4YX-ray3.20D1/d11-87[»]
4U4ZX-ray3.10D1/d11-87[»]
4U50X-ray3.20D1/d11-87[»]
4U51X-ray3.20D1/d11-87[»]
4U52X-ray3.00D1/d11-87[»]
4U53X-ray3.30D1/d11-87[»]
4U55X-ray3.20D1/d11-87[»]
4U56X-ray3.45D1/d11-87[»]
4U6FX-ray3.10D1/d11-87[»]
4V6Ielectron microscopy8.80AT1-87[»]
4V88X-ray3.00AV/CV1-87[»]
4V8Yelectron microscopy4.30AV1-87[»]
4V8Zelectron microscopy6.60AV1-87[»]
4V92electron microscopy3.70V1-87[»]
5DATX-ray3.15D1/d11-87[»]
5DC3X-ray3.25D1/d11-87[»]
5FCIX-ray3.40D1/d11-87[»]
5FCJX-ray3.10D1/d11-87[»]
5I4LX-ray3.10D1/d11-87[»]
5JUOelectron microscopy4.00SB1-87[»]
5JUPelectron microscopy3.50SB1-87[»]
5JUSelectron microscopy4.20SB1-87[»]
5JUTelectron microscopy4.00SB1-87[»]
5JUUelectron microscopy4.00SB1-87[»]
ProteinModelPortaliP0C0V8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34188. 59 interactors.
IntActiP0C0V8. 8 interactors.
MINTiMINT-4493667.

PTM databases

iPTMnetiP0C0V8.

2D gel databases

UCD-2DPAGEP0C0V8.

Proteomic databases

MaxQBiP0C0V8.
PRIDEiP0C0V8.
TopDownProteomicsiP0C0V8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR057W; YKR057W; YKR057W.
GeneIDi853931.
KEGGisce:YKR057W.

Organism-specific databases

EuPathDBiFungiDB:YKR057W.
SGDiS000001765. RPS21A.

Phylogenomic databases

GeneTreeiENSGT00390000017515.
HOGENOMiHOG000183557.
InParanoidiP0C0V8.
KOiK02971.
OMAiTRYAICG.
OrthoDBiEOG092C5XGV.

Enzyme and pathway databases

BioCyciYEAST:G3O-32026-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiP0C0V8.

Family and domain databases

InterProiIPR001931. Ribosomal_S21e.
IPR018279. Ribosomal_S21e_CS.
[Graphical view]
PANTHERiPTHR10442. PTHR10442. 1 hit.
PfamiPF01249. Ribosomal_S21e. 1 hit.
[Graphical view]
PIRSFiPIRSF002148. Ribosomal_S21e. 1 hit.
ProDomiPD006584. Ribosomal_S21e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00996. RIBOSOMAL_S21E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRS21A_YEAST
AccessioniPrimary (citable) accession number: P0C0V8
Secondary accession number(s): D6VXB8, P05760
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 30, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 27400 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S21 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.