P0C0U4 (RIMK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal protein S6 modification protein

Gene names

Name:	rimK
Ordered Locus Names:	b0852, JW0836

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6. HAMAP MF_01552
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	Belongs to the rimK family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Ribosomal protein S6 modification protein HAMAP MF_01552		PRO_0000205455
Regions
	Domain	104 – 287	184	ATP-grasp
	Nucleotide binding	131 – 186	56	ATP By similarity
Sites
	Metal binding	248	1	Magnesium or manganese 1 By similarity
	Metal binding	260	1	Magnesium or manganese 1 By similarity
	Metal binding	260	1	Magnesium or manganese 2 By similarity
	Metal binding	262	1	Magnesium or manganese 2 By similarity
Experimental info
	Sequence conflict	1 – 18	18	MKIAI…SCKRL → MERSIRVSGW Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0C0U4 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: C0AF021292ED41DD	FASTA	300	32,436
	10 20 30 40 50 60 MKIAILSRDG TLYSCKRLRE AAIQRGHLVE ILDPLSCYMN INPAASSIHY KGRKLPHFDA 70 80 90 100 110 120 VIPRIGTAIT FYGTAALRQF EMLGSYPLNE SVAIARARDK LRSMQLLARQ GIDLPVTGIA 130 140 150 160 170 180 HSPDDTSDLI DMVGGAPLVV KLVEGTQGIG VVLAETRQAA ESVIDAFRGL NAHILVQEYI 190 200 210 220 230 240 KEAQGCDIRC LVVGDEVVAA IERRAKEGDF RSNLHRGGAA SVASITPQER EIAIKAARTM 250 260 270 280 290 300 ALDVAGVDIL RANRGPLVME VNASPGLEGI EKTTGIDIAG KMIRWIERHA TTEYCLKTGG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the gene rimK responsible for the addition of glutamic acid residues to the C-terminus of ribosomal protein S6 in Escherichia coli K12." Kang W.-K., Icho T., Isono S., Kitakawa M., Isono K. Mol. Gen. Genet. 217:281-288(1989) [PubMed: 2570347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15859 Genomic DNA. Translation: CAA33868.1. U00096 Genomic DNA. Translation: AAC73939.1. AP009048 Genomic DNA. Translation: BAA35563.2.
PIR	D64823.
RefSeq	NP_415373.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0C0U4.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0C0U4. 4 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002883; EBESCP00000002883; EBESCG00000002352. EBESCT00000002884; EBESCP00000002884; EBESCG00000002352. EBESCT00000014809; EBESCP00000014100; EBESCG00000013869.
GeneID	945484.
GenomeReviews	Gene locus JW0836 in contig AP009048_GR. Gene locus b0852 in contig U00096_GR.
KEGG	ecj:JW0836. eco:b0852.
PATRIC	32116909. VBIEscCol129921_0880.
Organism-specific databases
EchoBASE	EB0845.
EcoGene	EG10852. rimK.
Phylogenomic databases
eggNOG	COG0189.
GeneTree	EBGT00050000009191.
HOGENOM	HBG679591.
OMA	CREPRLY.
PhylomeDB	P0C0U4.
ProtClustDB	PRK10446.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10852-MONOMER.
Gene expression databases
Genevestigator	P0C0U4.
Family and domain databases
HAMAP	MF_01552. RimK. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013651. ATP-grasp_RimK-type. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR023533. Ribosomal_S6_modifier_RimK. IPR004666. RpS6_RimK/Lys_biosynth_LsyX. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
KO	K05844.
Pfam	PF08443. RimK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00768. RimK_fam. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIMK_ECOLI

Accession

Primary (citable) accession number: P0C0U4
Secondary accession number(s): P17116, P75814

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program