Protein PB1-F2 - Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)

Contribute

Send feedback

Read comments (?) or add your own

P0C0U1 (PB1F2_I34A1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 46. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein PB1-F2

Gene names

Name:

PB1

Organism

Influenza A virus (strain A/Puerto Rico/8/1934 H1N1) [Reference proteome]

Taxonomic identifier

211044 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A ›

Virus host

Aves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in promoting lung pathology in both primary viral infection and secondary bacterial infection. Promotes alteration of mitochondrial morphology, dissipation of mitochondrial membrane potential, and cell death. Alternatively, inhibits the production of interferon in the infected cell at the level of host mitochondrial antiviral signaling MAVS. Its level of expression differs greatly depending on which cell type is infected, in a manner that is independent of the levels of expression of other viral proteins. Monocytic cells are more affected than epithelial cells. Seems to disable virus-infected monocytes or other host innate immune cells. May also act in trans: extracellular PB1-F2 released by infected cells could potentially inactivate hosts cell recruitment to the site of infection. During early stage of infection, may predispose the mitochondria to permeability transition through interaction with human SLC25A6/ANT3 and VDAC1. These proteins participate in the formation of the permeability transition pore complex (PTPC) responsible of the release of mitochondrial products that triggers apoptosis. Ref.8
Subunit structure	Oligomer. Interacts with human SLC25A6/ANT3 and VDAC1. Ref.6
Subcellular location	Host mitochondrion inner membrane. Host nucleus. Host cytoplasm › host cytosol. Note: Inner mitochondrial membrane in most cells types. Otherwise is detected in the nucleus and cytosol. Ref.5
Miscellaneous	Is not encoded in all strains, and seems to be dispensable for replication.
Sequence similarities	Belongs to the influenza viruses PB1-F2 family.

Ontologies

Keywords
Biological process	Apoptosis Host-virus interaction Inhibition of host IFN-mediated response initiation by virus Inhibition of host MAVS by virus Inhibition of host innate immune response by virus Modulation of host cell apoptosis by virus Viral immunoevasion
Cellular component	Host cytoplasm Host membrane Host mitochondrion Host mitochondrion inner membrane Host nucleus Membrane
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	active induction of host immune response by virus Traceable author statement. Source: Reactome apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW modulation by virus of host apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host MAVS activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon production Inferred from electronic annotation. Source: UniProtKB-KW viral transcription Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome host cell cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell host cell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 87

Protein PB1-F2

PRO_0000078743

Regions

Region

65 – 87

Mitochondrial targeting sequence

Sites

Site

Important for pathogenicity, S is highly pathogenic whereas N is low pathogenic By similarity

Natural variations

Natural variant

Q → E.

Natural variant

59 – 60

KQ → RR.

Secondary structure

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0C0U1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 832A7E34E350B454
Show »

FASTA

10,483

        10         20         30         40         50         60 
MGQEQDTPWI LSTGHISTQK RQDGQQTPKL EHRNSTRLMG HCQKTMNQVV MPKQIVYWKQ 

        70         80 
WLSLRNPILV FLKTRVLKRW RLFSKHE

« Hide

References

[1]	"Nucleotide sequence of human influenza A/PR/8/34 segment 2." Winter G., Fields S. Nucleic Acids Res. 10:2135-2143(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments." de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M. Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
[3]	"The NIAID influenza genome sequencing project." Ghedin E., Spiro D., Miller N., Zaborsky J., Feldblyum T., Subbu V., Shumway M., Sparenborg J., Groveman L., Halpin R., Sitz J., Koo H., Salzberg S.L., Webster R.G., Hoffmann E., Krauss S., Naeve C., Bao Y. Tatusova T. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[4]	"A novel influenza A virus mitochondrial protein that induces cell death." Chen W., Calvo P.A., Malide D., Gibbs J., Schubert U., Bacik I., Basta S., O'Neill R., Schickli J., Palese P., Henklein P., Bennink J.R., Yewdell J.W. Nat. Med. 7:1306-1312(2001) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"The influenza A virus PB1-F2 protein targets the inner mitochondrial membrane via a predicted basic amphipathic helix that disrupts mitochondrial function." Gibbs J.S., Malide D., Hornung F., Bennink J.R., Yewdell J.W. J. Virol. 77:7214-7224(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Influenza virus PB1-F2 protein induces cell death through mitochondrial ANT3 and VDAC1." Zamarin D., Garcia-Sastre A., Xiao X., Wang R., Palese P. PLoS Pathog. 1:40-54(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HUMAN SLC25A6/ANT3 AND VDAC1.
[7]	"Structural characterization and oligomerization of PB1-F2, a proapoptotic Influenza A Virus protein." Bruns K., Studtrucker N., Sharma A., Fossen T., Mitzner D., Eissmann A., Tessmer U., Roder R., Henklein P., Wray V., Schubert U. J. Biol. Chem. 282:353-363(2007) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 50-87.
[8]	"The influenza virus protein PB1-F2 inhibits the induction of type I interferon at the level of the MAVS adaptor protein." Varga Z.T., Ramos I., Hai R., Schmolke M., Garcia-Sastre A., Fernandez-Sesma A., Palese P. PLoS Pathog. 7:E1002067-E1002067(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J02151 Genomic RNA. No translation available.
EF467819 Genomic RNA. Translation: ABO21707.1.
CY009450 Genomic RNA. Translation: ABD77684.1.

RefSeq

YP_418248.1. NC_002021.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HN8	NMR	-	A	50-87	[»]
3BUY	X-ray	2.60	C	62-70	[»]

ProteinModelPortal

P0C0U1.

SMR

P0C0U1. Positions 50-87.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3802042.

Enzyme and pathway databases

Reactome

REACT_116125. Disease.

Family and domain databases

InterPro

IPR021045. Flu_proapoptotic_PB1-F2.
[Graphical view]

Pfam

PF11986. PB1-F2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0C0U1.

Entry information

Entry name

PB1F2_I34A1

Accession

Primary (citable) accession number: P0C0U1
Secondary accession number(s): A4GXH2, Q20N29

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents