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Protein

Protein PB1-F2

Gene

PB1

Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in promoting lung pathology in both primary viral infection and secondary bacterial infection. Promotes alteration of mitochondrial morphology, dissipation of mitochondrial membrane potential, and cell death. Alternatively, inhibits the production of interferon in the infected cell at the level of host mitochondrial antiviral signaling MAVS. Its level of expression differs greatly depending on which cell type is infected, in a manner that is independent of the levels of expression of other viral proteins. Monocytic cells are more affected than epithelial cells. Seems to disable virus-infected monocytes or other host innate immune cells. May also act in trans: extracellular PB1-F2 released by infected cells could potentially inactivate hosts cell recruitment to the site of infection. During early stage of infection, may predispose the mitochondria to permeability transition through interaction with human SLC25A6/ANT3 and VDAC1. These proteins participate in the formation of the permeability transition pore complex (PTPC) responsible of the release of mitochondrial products that triggers apoptosis.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei66 – 661Important for pathogenicity, S is highly pathogenic whereas N is low pathogenicBy similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Modulation of host cell apoptosis by virus, Viral immunoevasion

Enzyme and pathway databases

ReactomeiREACT_6213. Influenza Virus Induced Apoptosis.
REACT_9491. Viral mRNA Translation.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PB1-F2
Gene namesi
Name:PB1
OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Taxonomic identifieri211044 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000009255 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Host mitochondrion, Host mitochondrion inner membrane, Host nucleus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8787Protein PB1-F2PRO_0000078743Add
BLAST

Interactioni

Subunit structurei

Oligomer. Interacts with human SLC25A6/ANT3 and VDAC1.1 Publication

Structurei

Secondary structure

1
87
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi52 – 6211Combined sources
Helixi65 – 8622Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HN8NMR-A50-87[»]
3BUYX-ray2.60C62-70[»]
ProteinModelPortaliP0C0U1.
SMRiP0C0U1. Positions 50-87.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0U1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni65 – 8723Mitochondrial targeting sequenceAdd
BLAST

Sequence similaritiesi

Belongs to the influenza viruses PB1-F2 family.Curated

Family and domain databases

InterProiIPR021045. Flu_proapoptotic_PB1-F2.
[Graphical view]
PfamiPF11986. PB1-F2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C0U1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQEQDTPWI LSTGHISTQK RQDGQQTPKL EHRNSTRLMG HCQKTMNQVV
60 70 80
MPKQIVYWKQ WLSLRNPILV FLKTRVLKRW RLFSKHE
Length:87
Mass (Da):10,483
Last modified:December 6, 2005 - v1
Checksum:i832A7E34E350B454
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti22 – 221Q → E.
Natural varianti59 – 602KQ → RR.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02151 Genomic RNA. No translation available.
EF467819 Genomic RNA. Translation: ABO21707.1.
CY009450 Genomic RNA. Translation: ABD77684.1.
RefSeqiYP_418248.1. NC_002021.1.

Genome annotation databases

GeneIDi3802042.
KEGGivg:3802042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02151 Genomic RNA. No translation available.
EF467819 Genomic RNA. Translation: ABO21707.1.
CY009450 Genomic RNA. Translation: ABD77684.1.
RefSeqiYP_418248.1. NC_002021.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HN8NMR-A50-87[»]
3BUYX-ray2.60C62-70[»]
ProteinModelPortaliP0C0U1.
SMRiP0C0U1. Positions 50-87.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3802042.
KEGGivg:3802042.

Enzyme and pathway databases

ReactomeiREACT_6213. Influenza Virus Induced Apoptosis.
REACT_9491. Viral mRNA Translation.

Miscellaneous databases

EvolutionaryTraceiP0C0U1.

Family and domain databases

InterProiIPR021045. Flu_proapoptotic_PB1-F2.
[Graphical view]
PfamiPF11986. PB1-F2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of human influenza A/PR/8/34 segment 2."
    Winter G., Fields S.
    Nucleic Acids Res. 10:2135-2143(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments."
    de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.
    Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  4. Cited for: CHARACTERIZATION.
  5. "The influenza A virus PB1-F2 protein targets the inner mitochondrial membrane via a predicted basic amphipathic helix that disrupts mitochondrial function."
    Gibbs J.S., Malide D., Hornung F., Bennink J.R., Yewdell J.W.
    J. Virol. 77:7214-7224(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Influenza virus PB1-F2 protein induces cell death through mitochondrial ANT3 and VDAC1."
    Zamarin D., Garcia-Sastre A., Xiao X., Wang R., Palese P.
    PLoS Pathog. 1:40-54(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN SLC25A6/ANT3 AND VDAC1.
  7. "Structural characterization and oligomerization of PB1-F2, a proapoptotic Influenza A Virus protein."
    Bruns K., Studtrucker N., Sharma A., Fossen T., Mitzner D., Eissmann A., Tessmer U., Roder R., Henklein P., Wray V., Schubert U.
    J. Biol. Chem. 282:353-363(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 50-87.
  8. "The influenza virus protein PB1-F2 inhibits the induction of type I interferon at the level of the MAVS adaptor protein."
    Varga Z.T., Ramos I., Hai R., Schmolke M., Garcia-Sastre A., Fernandez-Sesma A., Palese P.
    PLoS Pathog. 7:E1002067-E1002067(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPB1F2_I34A1
AccessioniPrimary (citable) accession number: P0C0U1
Secondary accession number(s): A4GXH2, Q20N29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 24, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Is not encoded in all strains, and seems to be dispensable for replication.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.