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P0C0U1

- PB1F2_I34A1

UniProt

P0C0U1 - PB1F2_I34A1

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Protein
Protein PB1-F2
Gene
PB1
Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays an important role in promoting lung pathology in both primary viral infection and secondary bacterial infection. Promotes alteration of mitochondrial morphology, dissipation of mitochondrial membrane potential, and cell death. Alternatively, inhibits the production of interferon in the infected cell at the level of host mitochondrial antiviral signaling MAVS. Its level of expression differs greatly depending on which cell type is infected, in a manner that is independent of the levels of expression of other viral proteins. Monocytic cells are more affected than epithelial cells. Seems to disable virus-infected monocytes or other host innate immune cells. May also act in trans: extracellular PB1-F2 released by infected cells could potentially inactivate hosts cell recruitment to the site of infection. During early stage of infection, may predispose the mitochondria to permeability transition through interaction with human SLC25A6/ANT3 and VDAC1. These proteins participate in the formation of the permeability transition pore complex (PTPC) responsible of the release of mitochondrial products that triggers apoptosis.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei66 – 661Important for pathogenicity, S is highly pathogenic whereas N is low pathogenic By similarity

GO - Biological processi

  1. active induction of host immune response by virus Source: Reactome
  2. apoptotic process Source: UniProtKB-KW
  3. modulation by virus of host apoptotic process Source: UniProtKB-KW
  4. modulation by virus of host morphology or physiology Source: Reactome
  5. negative regulation of interferon-beta secretion Source: CACAO
  6. suppression by virus of host MAVS activity Source: UniProtKB
  7. viral life cycle Source: Reactome
  8. viral process Source: Reactome
  9. viral transcription Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Modulation of host cell apoptosis by virus, Viral immunoevasion

Enzyme and pathway databases

ReactomeiREACT_6213. Influenza Virus Induced Apoptosis.
REACT_9491. Viral mRNA Translation.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PB1-F2
Gene namesi
Name:PB1
OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Taxonomic identifieri211044 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000009255: Genome

Subcellular locationi

Host mitochondrion inner membrane. Host nucleus. Host cytoplasmhost cytosol
Note: Inner mitochondrial membrane in most cells types. Otherwise is detected in the nucleus and cytosol.1 Publication

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. host cell cytosol Source: UniProtKB-SubCell
  3. host cell mitochondrial inner membrane Source: UniProtKB-SubCell
  4. host cell nucleus Source: UniProtKB-SubCell
  5. membrane Source: UniProtKB-KW
  6. mitochondrial intermembrane space Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Host mitochondrion, Host mitochondrion inner membrane, Host nucleus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8787Protein PB1-F2
PRO_0000078743Add
BLAST

Interactioni

Subunit structurei

Oligomer. Interacts with human SLC25A6/ANT3 and VDAC1.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi52 – 6211
Helixi65 – 8622

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HN8NMR-A50-87[»]
3BUYX-ray2.60C62-70[»]
ProteinModelPortaliP0C0U1.
SMRiP0C0U1. Positions 50-87.

Miscellaneous databases

EvolutionaryTraceiP0C0U1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni65 – 8723Mitochondrial targeting sequence
Add
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR021045. Flu_proapoptotic_PB1-F2.
[Graphical view]
PfamiPF11986. PB1-F2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C0U1-1 [UniParc]FASTAAdd to Basket

« Hide

MGQEQDTPWI LSTGHISTQK RQDGQQTPKL EHRNSTRLMG HCQKTMNQVV   50
MPKQIVYWKQ WLSLRNPILV FLKTRVLKRW RLFSKHE 87
Length:87
Mass (Da):10,483
Last modified:December 6, 2005 - v1
Checksum:i832A7E34E350B454
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti22 – 221Q → E.
Natural varianti59 – 602KQ → RR.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02151 Genomic RNA. No translation available.
EF467819 Genomic RNA. Translation: ABO21707.1.
CY009450 Genomic RNA. Translation: ABD77684.1.
RefSeqiYP_418248.1. NC_002021.1.

Genome annotation databases

GeneIDi3802042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02151 Genomic RNA. No translation available.
EF467819 Genomic RNA. Translation: ABO21707.1 .
CY009450 Genomic RNA. Translation: ABD77684.1 .
RefSeqi YP_418248.1. NC_002021.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2HN8 NMR - A 50-87 [» ]
3BUY X-ray 2.60 C 62-70 [» ]
ProteinModelPortali P0C0U1.
SMRi P0C0U1. Positions 50-87.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3802042.

Enzyme and pathway databases

Reactomei REACT_6213. Influenza Virus Induced Apoptosis.
REACT_9491. Viral mRNA Translation.

Miscellaneous databases

EvolutionaryTracei P0C0U1.

Family and domain databases

InterProi IPR021045. Flu_proapoptotic_PB1-F2.
[Graphical view ]
Pfami PF11986. PB1-F2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of human influenza A/PR/8/34 segment 2."
    Winter G., Fields S.
    Nucleic Acids Res. 10:2135-2143(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments."
    de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.
    Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  4. Cited for: CHARACTERIZATION.
  5. "The influenza A virus PB1-F2 protein targets the inner mitochondrial membrane via a predicted basic amphipathic helix that disrupts mitochondrial function."
    Gibbs J.S., Malide D., Hornung F., Bennink J.R., Yewdell J.W.
    J. Virol. 77:7214-7224(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Influenza virus PB1-F2 protein induces cell death through mitochondrial ANT3 and VDAC1."
    Zamarin D., Garcia-Sastre A., Xiao X., Wang R., Palese P.
    PLoS Pathog. 1:40-54(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN SLC25A6/ANT3 AND VDAC1.
  7. "Structural characterization and oligomerization of PB1-F2, a proapoptotic Influenza A Virus protein."
    Bruns K., Studtrucker N., Sharma A., Fossen T., Mitzner D., Eissmann A., Tessmer U., Roder R., Henklein P., Wray V., Schubert U.
    J. Biol. Chem. 282:353-363(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 50-87.
  8. "The influenza virus protein PB1-F2 inhibits the induction of type I interferon at the level of the MAVS adaptor protein."
    Varga Z.T., Ramos I., Hai R., Schmolke M., Garcia-Sastre A., Fernandez-Sesma A., Palese P.
    PLoS Pathog. 7:E1002067-E1002067(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPB1F2_I34A1
AccessioniPrimary (citable) accession number: P0C0U1
Secondary accession number(s): A4GXH2, Q20N29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Is not encoded in all strains, and seems to be dispensable for replication.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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