Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Penicillin-insensitive murein endopeptidase

Gene

mepA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Murein endopeptidase that cleaves the D-alanyl-meso-2,6-diamino-pimelyl amide bond that connects peptidoglycan strands. Likely plays a role in the removal of murein from the sacculus and could also play a role in the integration of nascent murein strands into the sacculus.1 Publication

Miscellaneous

In E.coli there are three murein endopeptidases: two are penicillin sensitive (DacB and PbpG), the other (MepA) not.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit. Zn2+ ion 1 is bound in the active site. Zn2+ ion 2 is bound at the dimer interface by residues from both subunits.1 Publication

Enzyme regulationi

Inhibited by Zn2+ at 10 mM and by metal chelating agents EDTA and 1,10-phenanthroline.1 Publication

pH dependencei

Optimum pH is 5-8.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi110Zinc 11 Publication1
Metal bindingi113Zinc 11 Publication1
Metal bindingi120Zinc 11 Publication1
Metal bindingi147Zinc 21 Publication1
Metal bindingi150Zinc 21 Publication1
Metal bindingi211Zinc 11 Publication1

GO - Molecular functioni

  • endopeptidase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • metallopeptidase activity Source: UniProtKB-KW
  • peptidase activity Source: EcoCyc
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  • peptidoglycan biosynthetic process Source: EcoCyc
  • peptidoglycan metabolic process Source: EcoCyc
  • response to drug Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG10580-MONOMER
MetaCyc:EG10580-MONOMER

Protein family/group databases

MEROPSiM74.001

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-insensitive murein endopeptidase (EC:3.4.24.-1 Publication)
Alternative name(s):
D-alanyl-D-alanine-endopeptidase
Short name:
DD-endopeptidase
Gene namesi
Name:mepA
Ordered Locus Names:b2328, JW2325
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10580 mepA

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi113H → A: Strongly reduces enzyme activity. 1 Publication1
Mutagenesisi120D → A: Strongly reduces enzyme activity. 1 Publication1
Mutagenesisi209H → A: Strongly reduces enzyme activity. 1 Publication1
Mutagenesisi211H → A: Strongly reduces enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000002852020 – 274Penicillin-insensitive murein endopeptidaseAdd BLAST255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi44 ↔ 2651 Publication
Disulfide bondi187 ↔ 2351 Publication
Disulfide bondi216 ↔ 2231 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0C0T5
PRIDEiP0C0T5

Interactioni

Subunit structurei

Dimer.1 Publication

Protein-protein interaction databases

BioGridi4259647, 321 interactors
IntActiP0C0T5, 1 interactor
STRINGi316385.ECDH10B_2490

Structurei

Secondary structure

1274
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 25Combined sources4
Beta strandi35 – 39Combined sources5
Beta strandi42 – 47Combined sources6
Beta strandi56 – 61Combined sources6
Helixi63 – 65Combined sources3
Beta strandi68 – 70Combined sources3
Helixi72 – 87Combined sources16
Beta strandi93 – 95Combined sources3
Beta strandi107 – 109Combined sources3
Beta strandi118 – 123Combined sources6
Helixi132 – 136Combined sources5
Beta strandi148 – 151Combined sources4
Turni153 – 155Combined sources3
Helixi158 – 169Combined sources12
Beta strandi173 – 178Combined sources6
Helixi180 – 189Combined sources10
Helixi195 – 199Combined sources5
Beta strandi200 – 202Combined sources3
Beta strandi207 – 214Combined sources8
Helixi237 – 241Combined sources5
Helixi263 – 270Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TZPX-ray1.40A/B20-274[»]
1U10X-ray2.40A/B/C/D/E/F20-274[»]
ProteinModelPortaliP0C0T5
SMRiP0C0T5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0T5

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M74 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105HPZ Bacteria
COG3770 LUCA
HOGENOMiHOG000276210
InParanoidiP0C0T5
KOiK07261
OMAiVRPWWGH

Family and domain databases

Gene3Di3.30.1380.10, 1 hit
HAMAPiMF_01623 MepA, 1 hit
InterProiView protein in InterPro
IPR009045 Hedgehog_sig/DD-Pept_Zn-bd_sf
IPR005073 Peptidase_M74
PfamiView protein in Pfam
PF03411 Peptidase_M74, 1 hit
PIRSFiPIRSF018455 MepA, 1 hit
SUPFAMiSSF55166 SSF55166, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C0T5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKTAIALLA LLASSASLAA TPWQKITQPV PGSAQSIGSF SNGCIVGADT
60 70 80 90 100
LPIQSEHYQV MRTDQRRYFG HPDLVMFIQR LSSQVSNLGM GTVLIGDMGM
110 120 130 140 150
PAGGRFNGGH ASHQTGLDVD IFLQLPKTRW TSAQLLRPQA LDLVSRDGKH
160 170 180 190 200
VVSTLWKPEI FSLIKLAAQD KDVTRIFVNP AIKQQLCLDA GTDRDWLRKV
210 220 230 240 250
RPWFQHRAHM HVRLRCPADS LECEDQPLPP SGDGCGAELQ SWFEPPKPGT
260 270
TKPEKKTPPP LPPSCQALLD EHVI
Length:274
Mass (Da):30,137
Last modified:December 6, 2005 - v1
Checksum:iEF91AFD4B381019A
GO

Mass spectrometryi

Molecular mass is 28295 Da from positions 20 - 274. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16909 mRNA Translation: CAA34782.1
U00096 Genomic DNA Translation: AAC75388.1
AP009048 Genomic DNA Translation: BAA16184.1
PIRiS08345
RefSeqiNP_416831.1, NC_000913.3
WP_001043825.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75388; AAC75388; b2328
BAA16184; BAA16184; BAA16184
GeneIDi946812
KEGGiecj:JW2325
eco:b2328
PATRICifig|1411691.4.peg.4404

Entry informationi

Entry nameiMEPA_ECOLI
AccessioniPrimary (citable) accession number: P0C0T5
Secondary accession number(s): P14007
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health