ID   RS25B_YEAST             Reviewed;         108 AA.
AC   P0C0T4; A2TBM7; D6VYX4; P05758; P07282;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   27-MAR-2024, entry version 135.
DE   RecName: Full=Small ribosomal subunit protein eS25B {ECO:0000303|PubMed:24524803};
DE   AltName: Full=40S ribosomal protein S25-B {ECO:0000303|PubMed:9559554};
DE   AltName: Full=RP45;
DE   AltName: Full=S31;
DE   AltName: Full=YS23;
GN   Name=RPS25B {ECO:0000303|PubMed:9559554}; Synonyms=RPS31, RPS31B;
GN   OrderedLocusNames=YLR333C; ORFNames=L8300.10;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA   Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA   Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA   Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA   Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA   Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA   Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA   Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA   Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA   Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA   Zollner A., Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-62.
RC   STRAIN=ATCC 201390 / BY4743;
RX   PubMed=17244705; DOI=10.1073/pnas.0610354104;
RA   Juneau K., Palm C., Miranda M., Davis R.W.;
RT   "High-density yeast-tiling array reveals previously undiscovered introns
RT   and extensive regulation of meiotic splicing.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007).
RN   [5]
RP   PARTIAL PROTEIN SEQUENCE OF 15-22.
RX   PubMed=18782943; DOI=10.1007/bf00341461;
RA   Otaka E., Higo K., Itoh T.;
RT   "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence
RT   characterization of twenty-four proteins from cytoplasmic ribosomes.";
RL   Mol. Gen. Genet. 195:544-546(1984).
RN   [6]
RP   NOMENCLATURE, AND SUBUNIT.
RX   PubMed=9559554;
RX   DOI=10.1002/(sici)1097-0061(19980330)14:5<471::aid-yea241>3.0.co;2-u;
RA   Planta R.J., Mager W.H.;
RT   "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae.";
RL   Yeast 14:471-477(1998).
RN   [7]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [8]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [9]
RP   METHYLATION AT PRO-2 BY NTM1/TAE1.
RX   PubMed=20481588; DOI=10.1021/bi100428x;
RA   Webb K.J., Lipson R.S., Al-Hadid Q., Whitelegge J.P., Clarke S.G.;
RT   "Identification of protein N-terminal methyltransferases in yeast and
RT   humans.";
RL   Biochemistry 49:5225-5235(2010).
RN   [10]
RP   SUBUNIT, AND SUBCELLULAR LOCATION.
RX   PubMed=22096102; DOI=10.1126/science.1212642;
RA   Ben-Shem A., Garreau de Loubresse N., Melnikov S., Jenner L., Yusupova G.,
RA   Yusupov M.;
RT   "The structure of the eukaryotic ribosome at 3.0 A resolution.";
RL   Science 334:1524-1529(2011).
RN   [11]
RP   NOMENCLATURE.
RX   PubMed=24524803; DOI=10.1016/j.sbi.2014.01.002;
RA   Ban N., Beckmann R., Cate J.H.D., Dinman J.D., Dragon F., Ellis S.R.,
RA   Lafontaine D.L.J., Lindahl L., Liljas A., Lipton J.M., McAlear M.A.,
RA   Moore P.B., Noller H.F., Ortega J., Panse V.G., Ramakrishnan V.,
RA   Spahn C.M.T., Steitz T.A., Tchorzewski M., Tollervey D., Warren A.J.,
RA   Williamson J.R., Wilson D., Yonath A., Yusupov M.;
RT   "A new system for naming ribosomal proteins.";
RL   Curr. Opin. Struct. Biol. 24:165-169(2014).
CC   -!- FUNCTION: Component of the ribosome, a large ribonucleoprotein complex
CC       responsible for the synthesis of proteins in the cell. The small
CC       ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the
CC       encoded message by selecting cognate aminoacyl-transfer RNA (tRNA)
CC       molecules. The large subunit (LSU) contains the ribosomal catalytic
CC       site termed the peptidyl transferase center (PTC), which catalyzes the
CC       formation of peptide bonds, thereby polymerizing the amino acids
CC       delivered by tRNAs into a polypeptide chain. The nascent polypeptides
CC       leave the ribosome through a tunnel in the LSU and interact with
CC       protein factors that function in enzymatic processing, targeting, and
CC       the membrane insertion of nascent chains at the exit of the ribosomal
CC       tunnel. {ECO:0000305|PubMed:22096102}.
CC   -!- SUBUNIT: Component of the small ribosomal subunit (SSU). Mature yeast
CC       ribosomes consist of a small (40S) and a large (60S) subunit. The 40S
CC       small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33
CC       different proteins (encoded by 57 genes). The large 60S subunit
CC       contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different
CC       proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).
CC       {ECO:0000269|PubMed:22096102, ECO:0000305|PubMed:9559554}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095,
CC       ECO:0000269|PubMed:22096102}.
CC   -!- MISCELLANEOUS: It is presumed that the precursor part of S25 is engaged
CC       in assembling of the small subunit, thus being essential in ribosome
CC       maturation.
CC   -!- MISCELLANEOUS: Present with 162000 molecules/cell in log phase SD
CC       medium. {ECO:0000269|PubMed:14562106}.
CC   -!- MISCELLANEOUS: There are 2 genes for eS25 in yeast. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the eukaryotic ribosomal protein eS25 family.
CC       {ECO:0000305}.
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DR   EMBL; U19028; AAB67260.1; -; Genomic_DNA.
DR   EMBL; AY693146; AAT93165.1; -; Genomic_DNA.
DR   EMBL; EF123130; ABM97474.1; -; mRNA.
DR   EMBL; BK006945; DAA09640.1; -; Genomic_DNA.
DR   PIR; S51338; S51338.
DR   RefSeq; NP_013437.1; NM_001182222.1.
DR   AlphaFoldDB; P0C0T4; -.
DR   SMR; P0C0T4; -.
DR   BioGRID; 31597; 141.
DR   DIP; DIP-5077N; -.
DR   IntAct; P0C0T4; 8.
DR   MINT; P0C0T4; -.
DR   STRING; 4932.YLR333C; -.
DR   iPTMnet; P0C0T4; -.
DR   MaxQB; P0C0T4; -.
DR   PaxDb; 4932-YLR333C; -.
DR   PeptideAtlas; P0C0T4; -.
DR   EnsemblFungi; YLR333C_mRNA; YLR333C; YLR333C.
DR   GeneID; 851045; -.
DR   KEGG; sce:YLR333C; -.
DR   AGR; SGD:S000004325; -.
DR   SGD; S000004325; RPS25B.
DR   VEuPathDB; FungiDB:YLR333C; -.
DR   eggNOG; KOG1767; Eukaryota.
DR   GeneTree; ENSGT00390000004856; -.
DR   HOGENOM; CLU_129470_4_0_1; -.
DR   InParanoid; P0C0T4; -.
DR   OMA; IRMISSH; -.
DR   OrthoDB; 276079at2759; -.
DR   BioCyc; YEAST:G3O-32413-MONOMER; -.
DR   Reactome; R-SCE-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR   Reactome; R-SCE-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR   Reactome; R-SCE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR   Reactome; R-SCE-72649; Translation initiation complex formation.
DR   Reactome; R-SCE-72689; Formation of a pool of free 40S subunits.
DR   Reactome; R-SCE-72695; Formation of the ternary complex, and subsequently, the 43S complex.
DR   Reactome; R-SCE-72702; Ribosomal scanning and start codon recognition.
DR   Reactome; R-SCE-72706; GTP hydrolysis and joining of the 60S ribosomal subunit.
DR   Reactome; R-SCE-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR   Reactome; R-SCE-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR   BioGRID-ORCS; 851045; 2 hits in 10 CRISPR screens.
DR   PRO; PR:P0C0T4; -.
DR   Proteomes; UP000002311; Chromosome XII.
DR   RNAct; P0C0T4; Protein.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:SGD.
DR   GO; GO:0003735; F:structural constituent of ribosome; IDA:SGD.
DR   GO; GO:0002181; P:cytoplasmic translation; IC:SGD.
DR   Gene3D; 3.30.63.20; -; 1.
DR   InterPro; IPR004977; Ribosomal_eS25.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12850; 40S RIBOSOMAL PROTEIN S25; 1.
DR   PANTHER; PTHR12850:SF5; 40S RIBOSOMAL PROTEIN S25; 1.
DR   Pfam; PF03297; Ribosomal_S25; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Methylation; Reference proteome;
KW   Ribonucleoprotein; Ribosomal protein.
FT   INIT_MET        1
FT                   /note="Removed"
FT   CHAIN           2..108
FT                   /note="Small ribosomal subunit protein eS25B"
FT                   /id="PRO_0000030634"
FT   REGION          1..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         2
FT                   /note="N,N-dimethylproline; by NTM1"
FT                   /evidence="ECO:0000269|PubMed:20481588"
SQ   SEQUENCE   108 AA;  12009 MW;  C52D0334AEA125B5 CRC64;
     MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI LKEVPTYRYV
     SVSVLVDRLK IGGSLARIAL RHLEKEGIIK PISKHSKQAI YTRAAASE
//