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P0C0T4

- RS25B_YEAST

UniProt

P0C0T4 - RS25B_YEAST

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Protein

40S ribosomal protein S25-B

Gene

RPS25B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32413-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S25-B
Alternative name(s):
RP45
S31
YS23
Gene namesi
Name:RPS25B
Synonyms:RPS31, RPS31B
Ordered Locus Names:YLR333C
ORF Names:L8300.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR333c.
SGDiS000004325. RPS25B.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 10810740S ribosomal protein S25-BPRO_0000030634Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N,N-dimethylproline; by NTM11 Publication

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP0C0T4.

Expressioni

Gene expression databases

GenevestigatoriP0C0T4.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi31597. 29 interactions.
DIPiDIP-5077N.
IntActiP0C0T4. 7 interactions.
MINTiMINT-490537.

Structurei

3D structure databases

ProteinModelPortaliP0C0T4.
SMRiP0C0T4. Positions 36-104.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S25e family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000004856.
HOGENOMiHOG000203673.
InParanoidiP0C0T4.
KOiK02975.
OMAiHSVLFDK.
OrthoDBiEOG7647G6.

Family and domain databases

InterProiIPR004977. Ribosomal_S25.
[Graphical view]
PANTHERiPTHR12850. PTHR12850. 1 hit.
PfamiPF03297. Ribosomal_S25. 1 hit.
[Graphical view]
ProDomiPD012268. Ribosomal_S25. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C0T4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI
60 70 80 90 100
LKEVPTYRYV SVSVLVDRLK IGGSLARIAL RHLEKEGIIK PISKHSKQAI

YTRAAASE
Length:108
Mass (Da):12,009
Last modified:December 6, 2005 - v1
Checksum:iC52D0334AEA125B5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U19028 Genomic DNA. Translation: AAB67260.1.
AY693146 Genomic DNA. Translation: AAT93165.1.
EF123130 mRNA. Translation: ABM97474.1.
BK006945 Genomic DNA. Translation: DAA09640.1.
PIRiS51338.
RefSeqiNP_013437.1. NM_001182222.1.

Genome annotation databases

EnsemblFungiiYLR333C; YLR333C; YLR333C.
GeneIDi851045.
KEGGisce:YLR333C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U19028 Genomic DNA. Translation: AAB67260.1 .
AY693146 Genomic DNA. Translation: AAT93165.1 .
EF123130 mRNA. Translation: ABM97474.1 .
BK006945 Genomic DNA. Translation: DAA09640.1 .
PIRi S51338.
RefSeqi NP_013437.1. NM_001182222.1.

3D structure databases

ProteinModelPortali P0C0T4.
SMRi P0C0T4. Positions 36-104.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31597. 29 interactions.
DIPi DIP-5077N.
IntActi P0C0T4. 7 interactions.
MINTi MINT-490537.

Proteomic databases

MaxQBi P0C0T4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR333C ; YLR333C ; YLR333C .
GeneIDi 851045.
KEGGi sce:YLR333C.

Organism-specific databases

CYGDi YLR333c.
SGDi S000004325. RPS25B.

Phylogenomic databases

GeneTreei ENSGT00390000004856.
HOGENOMi HOG000203673.
InParanoidi P0C0T4.
KOi K02975.
OMAi HSVLFDK.
OrthoDBi EOG7647G6.

Enzyme and pathway databases

BioCyci YEAST:G3O-32413-MONOMER.
Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Miscellaneous databases

NextBioi 967653.

Gene expression databases

Genevestigatori P0C0T4.

Family and domain databases

InterProi IPR004977. Ribosomal_S25.
[Graphical view ]
PANTHERi PTHR12850. PTHR12850. 1 hit.
Pfami PF03297. Ribosomal_S25. 1 hit.
[Graphical view ]
ProDomi PD012268. Ribosomal_S25. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
    Juneau K., Palm C., Miranda M., Davis R.W.
    Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-62.
    Strain: ATCC 201390 / BY4743.
  5. "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
    Otaka E., Higo K., Itoh T.
    Mol. Gen. Genet. 195:544-546(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE OF 15-22.
  6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Identification of protein N-terminal methyltransferases in yeast and humans."
    Webb K.J., Lipson R.S., Al-Hadid Q., Whitelegge J.P., Clarke S.G.
    Biochemistry 49:5225-5235(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT PRO-2 BY NTM1/TAE1.

Entry informationi

Entry nameiRS25B_YEAST
AccessioniPrimary (citable) accession number: P0C0T4
Secondary accession number(s): A2TBM7
, D6VYX4, P05758, P07282
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: December 6, 2005
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

It is presumed that the precursor part of S25 is engaged in assembling of the small subunit, thus being essential in ribosome maturation.
Present with 162000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S25 in yeast.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3