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P0C0T4

- RS25B_YEAST

UniProt

P0C0T4 - RS25B_YEAST

Protein

40S ribosomal protein S25-B

Gene

RPS25B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32413-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein S25-B
    Alternative name(s):
    RP45
    S31
    YS23
    Gene namesi
    Name:RPS25B
    Synonyms:RPS31, RPS31B
    Ordered Locus Names:YLR333C
    ORF Names:L8300.10
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR333c.
    SGDiS000004325. RPS25B.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 10810740S ribosomal protein S25-BPRO_0000030634Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N,N-dimethylproline; by NTM11 Publication

    Keywords - PTMi

    Methylation

    Proteomic databases

    MaxQBiP0C0T4.

    Expressioni

    Gene expression databases

    GenevestigatoriP0C0T4.

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi31597. 29 interactions.
    DIPiDIP-5077N.
    IntActiP0C0T4. 7 interactions.
    MINTiMINT-490537.

    Structurei

    3D structure databases

    ProteinModelPortaliP0C0T4.
    SMRiP0C0T4. Positions 36-104.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein S25e family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000004856.
    HOGENOMiHOG000203673.
    KOiK02975.
    OMAiHSVLFDK.
    OrthoDBiEOG7647G6.

    Family and domain databases

    InterProiIPR004977. Ribosomal_S25.
    [Graphical view]
    PANTHERiPTHR12850. PTHR12850. 1 hit.
    PfamiPF03297. Ribosomal_S25. 1 hit.
    [Graphical view]
    ProDomiPD012268. Ribosomal_S25. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0C0T4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI    50
    LKEVPTYRYV SVSVLVDRLK IGGSLARIAL RHLEKEGIIK PISKHSKQAI 100
    YTRAAASE 108
    Length:108
    Mass (Da):12,009
    Last modified:December 6, 2005 - v1
    Checksum:iC52D0334AEA125B5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19028 Genomic DNA. Translation: AAB67260.1.
    AY693146 Genomic DNA. Translation: AAT93165.1.
    EF123130 mRNA. Translation: ABM97474.1.
    BK006945 Genomic DNA. Translation: DAA09640.1.
    PIRiS51338.
    RefSeqiNP_013437.1. NM_001182222.1.

    Genome annotation databases

    EnsemblFungiiYLR333C; YLR333C; YLR333C.
    GeneIDi851045.
    KEGGisce:YLR333C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19028 Genomic DNA. Translation: AAB67260.1 .
    AY693146 Genomic DNA. Translation: AAT93165.1 .
    EF123130 mRNA. Translation: ABM97474.1 .
    BK006945 Genomic DNA. Translation: DAA09640.1 .
    PIRi S51338.
    RefSeqi NP_013437.1. NM_001182222.1.

    3D structure databases

    ProteinModelPortali P0C0T4.
    SMRi P0C0T4. Positions 36-104.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31597. 29 interactions.
    DIPi DIP-5077N.
    IntActi P0C0T4. 7 interactions.
    MINTi MINT-490537.

    Proteomic databases

    MaxQBi P0C0T4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR333C ; YLR333C ; YLR333C .
    GeneIDi 851045.
    KEGGi sce:YLR333C.

    Organism-specific databases

    CYGDi YLR333c.
    SGDi S000004325. RPS25B.

    Phylogenomic databases

    GeneTreei ENSGT00390000004856.
    HOGENOMi HOG000203673.
    KOi K02975.
    OMAi HSVLFDK.
    OrthoDBi EOG7647G6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32413-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    NextBioi 967653.

    Gene expression databases

    Genevestigatori P0C0T4.

    Family and domain databases

    InterProi IPR004977. Ribosomal_S25.
    [Graphical view ]
    PANTHERi PTHR12850. PTHR12850. 1 hit.
    Pfami PF03297. Ribosomal_S25. 1 hit.
    [Graphical view ]
    ProDomi PD012268. Ribosomal_S25. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
      Juneau K., Palm C., Miranda M., Davis R.W.
      Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-62.
      Strain: ATCC 201390 / BY4743.
    5. "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
      Otaka E., Higo K., Itoh T.
      Mol. Gen. Genet. 195:544-546(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE OF 15-22.
    6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Identification of protein N-terminal methyltransferases in yeast and humans."
      Webb K.J., Lipson R.S., Al-Hadid Q., Whitelegge J.P., Clarke S.G.
      Biochemistry 49:5225-5235(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT PRO-2 BY NTM1/TAE1.

    Entry informationi

    Entry nameiRS25B_YEAST
    AccessioniPrimary (citable) accession number: P0C0T4
    Secondary accession number(s): A2TBM7
    , D6VYX4, P05758, P07282
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    It is presumed that the precursor part of S25 is engaged in assembling of the small subunit, thus being essential in ribosome maturation.
    Present with 162000 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for S25 in yeast.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Ribosomal proteins
      Ribosomal proteins families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3