Inositol-tetrakisphosphate 1-kinase

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P0C0T1 (ITPK1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 57. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Inositol-tetrakisphosphate 1-kinase
EC=2.7.1.134

Alternative name(s):
Inositol 1,3,4-trisphosphate 5/6-kinase
Short name=Inositol-triphosphate 5/6-kinase
Short name=Ins(1,3,4)P(3) 5/6-kinase
EC=2.7.1.159

Gene names

Name:

ITPK1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca²⁺-activated Cl^- channels, while Ins(1,3,4,5,6)P5 is not. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain By similarity. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway.
Catalytic activity	ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate. Ref.1 ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate. Ref.1 ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate.
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Subunit structure	Monomer. Interacts with GPS1/COPS1 By similarity.
Post-translational modification	Acetylation by EP300 and CREBBP destabilizes ITPK1, and down-regulates enzymatic activity. Deacetylated by SIRT1 By similarity.
Sequence similarities	Belongs to the ITPK1 family. Contains 1 ATP-grasp domain.
Biophysicochemical properties	Kinetic parameters: K_M=80 nM for Ins(1,3,4)P3 Ref.1 V_max=60 nmol/min/mg enzyme with Ins(1,3,4)P3 as substrate

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase Isomerase Kinase Transferase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	inositol trisphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW inositol tetrakisphosphate 1-kinase activity Inferred from electronic annotation. Source: EC inositol-1,3,4-trisphosphate 5-kinase activity Inferred from electronic annotation. Source: EC inositol-1,3,4-trisphosphate 6-kinase activity Inferred from electronic annotation. Source: EC isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 419

419

Inositol-tetrakisphosphate 1-kinase

PRO_0000220832

Regions

Domain

117 – 325

209

ATP-grasp

Nucleotide binding

188 – 199

ATP By similarity

Sites

Metal binding

281

Magnesium 1 By similarity

Metal binding

295

Magnesium 1 By similarity

Metal binding

295

Magnesium 2 By similarity

Metal binding

297

Magnesium 2 By similarity

Binding site

1D-myo-inositol 1,3,4-trisphosphate By similarity

Binding site

106

ATP By similarity

Binding site

157

ATP By similarity

Binding site

167

1D-myo-inositol 1,3,4-trisphosphate By similarity

Binding site

199

1D-myo-inositol 1,3,4-trisphosphate By similarity

Binding site

214

ATP By similarity

Binding site

232

ATP By similarity

Binding site

236

ATP By similarity

Binding site

297

1D-myo-inositol 1,3,4-trisphosphate By similarity

Amino acid modifications

Modified residue

388

N6-acetyllysine; by EP300 and CREBBP By similarity

Modified residue

415

N6-acetyllysine; by EP300 and CREBBP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P0C0T1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: FD4560C869A1A917
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FASTA

419

45,842

        10         20         30         40         50         60 
MQTFLKGKRV GYWLSEKKIK KLNFQAFAEL CRKRGIEVVQ LNLSRPIEEQ GPLDVIIHKL 

        70         80         90        100        110        120 
TDVILEADQN DSQALELVHR FQEYIDAHPE TIVLDPLPAI RTLLDRSKSY ELIRKIEAYM 

       130        140        150        160        170        180 
KDDRICSPPF MELTSLCGDD TMRLLEENGL AFPFICKTRV AHGTNSHEMA IVFNQEGLSA 

       190        200        210        220        230        240 
IQPPCVVQNF INHNAVLYKV FVVGESYTVV QRPSLKNFSA GTSDRESIFF NSHNVSKPES 

       250        260        270        280        290        300 
SSVLTALDKI EGVFERPSDE VIRELSRALR QALGVSLFGI DIIINNQTGQ HAVIDINAFP 

       310        320        330        340        350        360 
GYEGVSEFFT DLLNHIASVL QGQSSGVAGA GDVAPLKHSR LLAEQAGGLA AERTCSASPG 

       370        380        390        400        410 
CCSSMMGQEP PWTPEADMGG VGAGSTAKLP HQRLGCTAGV SPSFQQHCVA SLATKASSQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of inositol 1,3,4-trisphosphate 5/6-kinase, cDNA cloning and expression of the recombinant enzyme." Wilson M.P., Majerus P.W. J. Biol. Chem. 271:11904-11910(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-17; 200-211 AND 338-359, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. Tissue: Brain.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-245. Strain: Crossbred X Angus. Tissue: Ileum.
[3]	"Bovine ESTs: focus on female reproduction." Prather R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 203-419.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DT820636 mRNA. No translation available. CV982275 mRNA. No translation available.
IPI	IPI00718572.
RefSeq	NP_001179418.1. NM_001192489.1.
UniGene	Bt.62530.
3D structure databases
ProteinModelPortal	P0C0T1.
SMR	P0C0T1. Positions 6-322.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000042643.
Proteomic databases
PRIDE	P0C0T1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000045234; ENSBTAP00000042643; ENSBTAG00000009845.
GeneID	518488.
KEGG	bta:518488.
Organism-specific databases
CTD	3705.
Phylogenomic databases
eggNOG	NOG85132.
GeneTree	ENSGT00390000001278.
HOGENOM	HOG000007762.
HOVERGEN	HBG079462.
InParanoid	P0C0T1.
KO	K00913.
OMA	KDDRICS.
OrthoDB	EOG48D0VQ.
Family and domain databases
InterPro	IPR011761. ATP-grasp. IPR008656. Inositol_tetrakis-P_1-kinase. [Graphical view]
Pfam	PF05770. Ins134_P3_kin. 1 hit. [Graphical view]
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20872664.

Entry information

Entry name

ITPK1_BOVIN

Accession

Primary (citable) accession number: P0C0T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents