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Protein

Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog

Gene

Litaf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation. Plays a role in targeting endocytosed EGFR and ERGG3 for lysosomal degradation, and thereby helps downregulate downstream signaling cascades. Helps recruit the ESCRT complex components TSG101, HGS and STAM to cytoplasmic membranes. Probably plays a role in regulating protein degradation via its interaction with NEDD4. May also contribute to the regulation of gene expression in the nucleus. Binds DNA (in vitro) and may play a synergistic role with STAT6 in the nucleus in regulating the expression of various cytokines. May regulate the expression of numerous cytokines, such as TNF, CCL2, CCL5, CXCL1, IL1A and IL10.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi96ZincBy similarity1
Metal bindingi99ZincBy similarity1
Metal bindingi148ZincBy similarity1
Metal bindingi151ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
Short name:
LPS-induced TNF-alpha factor homolog
Alternative name(s):
Estrogen-enhanced transcript protein 1
Short name:
Eet-1
Gene namesi
Name:Litaf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi69294. Litaf.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Lysosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Early endosome membrane By similarity
  • Late endosome membrane By similarity
  • Endosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Golgi apparatus membrane By similarity

  • Note: Associated with membranes of lysosomes, early and late endosomes. Can translocate from the cytoplasm into the nucleus (By similarity). Detected at Schmidt-Lanterman incisures and in nodal regions of myelinating Schwann cells (By similarity).By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endosome, Golgi apparatus, Lysosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000844421 – 161Lipopolysaccharide-induced tumor necrosis factor-alpha factor homologAdd BLAST161

Post-translational modificationi

Phosphorylated on tyrosine residues in response to EGF.By similarity

Proteomic databases

PaxDbiP0C0T0.
PRIDEiP0C0T0.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Inductioni

By estrogen in vagina, cervix, uterus and kidney.1 Publication

Gene expression databases

BgeeiENSRNOG00000002520.
ExpressionAtlasiP0C0T0. baseline and differential.
GenevisibleiP0C0T0. RN.

Interactioni

Subunit structurei

Monomer. Interacts with NEDD4. Interacts (via PSAP motif) with TSG101, a component of the ESCRT-I complex (endosomal sorting complex required for transport I). Interacts with WWOX. Interacts with STAM, a component of the ESCRT-0 complex; the interaction is direct. Identified in a complex with STAM and HGS; within this complex, interacts directly with STAM, but not with HGS. Interacts with STAT6.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003412.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 159LITAFCuratedAdd BLAST70

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni111 – 134Membrane-binding amphipathic helixCuratedAdd BLAST24

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi20 – 23WW-bindingBy similarity4

Domaini

The WW-binding motif mediates interaction with WWOX and NEDD4.By similarity
The LITAF domain is stabilized by a bound zinc ion. The LITAF domain contains an amphiphatic helix that mediates interaction with lipid membranes. It interacts specifically with phosphatidylethanolamine lipid headgroups, but not with phosphoglycerol, phosphocholine, phosphoserine or inositolhexakisphosphate.By similarity

Sequence similaritiesi

Belongs to the CDIP1/LITAF family.Curated

Phylogenomic databases

eggNOGiENOG410IVVU. Eukaryota.
ENOG41122PJ. LUCA.
GeneTreeiENSGT00540000071542.
HOGENOMiHOG000039585.
HOVERGENiHBG006272.
InParanoidiP0C0T0.
KOiK19363.
OMAiHCHAEID.
OrthoDBiEOG091G0XNM.
PhylomeDBiP0C0T0.
TreeFamiTF313294.

Family and domain databases

InterProiView protein in InterPro
IPR006629. LITAF.
PfamiView protein in Pfam
PF10601. zf-LITAF-like. 1 hit.
SMARTiView protein in SMART
SM00714. LITAF. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C0T0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPGPYQAA AGPSVMPTAP PTYEETVGVN SYYPTPPAPQ PGPATGLITG
60 70 80 90 100
PDGKGMNPPS YYTQPVPVPN ANAIAVQTVY VQQPISFYDR PIQMCCPSCN
110 120 130 140 150
KMIVTQLSYN AGALTWLSCG SLCLLGCVAG CCFIPFCVDA LQDVDHYCPN
160
CKALLGTYKR L
Length:161
Mass (Da):17,030
Last modified:December 6, 2005 - v1
Checksum:i81DD842A4374F1FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53184 mRNA. No translation available.
RefSeqiNP_001099205.1. NM_001105735.2.
UniGeneiRn.6940.

Genome annotation databases

EnsembliENSRNOT00000003412; ENSRNOP00000003412; ENSRNOG00000002520.
GeneIDi65161.
KEGGirno:65161.
UCSCiRGD:69294. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLITAF_RAT
AccessioniPrimary (citable) accession number: P0C0T0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: July 5, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families