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Protein

Histone H2A.Z

Gene

H2afz

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to insulin stimulus Source: RGD
  • chromatin silencing Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2A.Z
Short name:
H2A/z
Gene namesi
Name:H2afz
Synonyms:H2az
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621464. H2afz.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromatin Source: GO_Central
  • nucleosome Source: UniProtKB-KW
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 128127Histone H2A.ZPRO_0000055300Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51N6-acetyllysine; alternateBy similarity
Modified residuei5 – 51N6-methyllysine; alternateBy similarity
Modified residuei8 – 81N6-acetyllysine; alternateBy similarity
Modified residuei8 – 81N6-methyllysine; alternateBy similarity
Modified residuei12 – 121N6-acetyllysineBy similarity
Modified residuei14 – 141N6-acetyllysineBy similarity
Cross-linki122 – 122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.By similarity
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis (By similarity).By similarity
Not phosphorylated.By similarity
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

PaxDbiP0C0S7.
PRIDEiP0C0S7.

PTM databases

iPTMnetiP0C0S7.
PhosphoSiteiP0C0S7.

Expressioni

Gene expression databases

BgeeiENSRNOG00000010306.

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms a heterodimer with H2B. H2AFZ interacts with INCENP. Interacts (via M6 cassette) with ANP32E; leading to removal of H2A.Z/H2AFZ from the nucleosome (By similarity).By similarity

Protein-protein interaction databases

BioGridi248676. 1 interaction.
STRINGi10116.ENSRNOP00000013919.

Structurei

3D structure databases

ProteinModelPortaliP0C0S7.
SMRiP0C0S7. Positions 17-123.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 1716Required for interaction with INCENPBy similarityAdd
BLAST
Regioni89 – 10012M6 cassetteBy similarityAdd
BLAST
Regioni93 – 10311Required for interaction with INCENPBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the histone H2A family.Curated

Phylogenomic databases

eggNOGiKOG1757. Eukaryota.
COG5262. LUCA.
HOGENOMiHOG000234652.
HOVERGENiHBG009342.
InParanoidiP0C0S7.
KOiK11251.
PhylomeDBiP0C0S7.
TreeFamiTF354232.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR002119. Histone_H2A.
IPR007125. Histone_H2A/H2B/H3.
IPR032454. Histone_H2A_C.
IPR032458. Histone_H2A_CS.
[Graphical view]
PfamiPF00125. Histone. 1 hit.
PF16211. Histone_H2A_C. 1 hit.
[Graphical view]
PRINTSiPR00620. HISTONEH2A.
SMARTiSM00414. H2A. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00046. HISTONE_H2A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C0S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGGKAGKDS GKAKTKAVSR SQRAGLQFPV GRIHRHLKSR TTSHGRVGAT
60 70 80 90 100
AAVYSAAILE YLTAEVLELA GNASKDLKVK RITPRHLQLA IRGDEELDSL
110 120
IKATIAGGGV IPHIHKSLIG KKGQQKTV
Length:128
Mass (Da):13,553
Last modified:January 23, 2007 - v2
Checksum:iE024E53818230371
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52316 mRNA. Translation: CAA36552.1.
M37584 mRNA. Translation: AAA41329.1.
BC060564 mRNA. Translation: AAH60564.1.
BC086348 mRNA. Translation: AAH86348.1.
PIRiS03644.
RefSeqiNP_073165.1. NM_022674.1.
UniGeneiRn.112573.

Genome annotation databases

GeneIDi58940.
KEGGirno:58940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52316 mRNA. Translation: CAA36552.1.
M37584 mRNA. Translation: AAA41329.1.
BC060564 mRNA. Translation: AAH60564.1.
BC086348 mRNA. Translation: AAH86348.1.
PIRiS03644.
RefSeqiNP_073165.1. NM_022674.1.
UniGeneiRn.112573.

3D structure databases

ProteinModelPortaliP0C0S7.
SMRiP0C0S7. Positions 17-123.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248676. 1 interaction.
STRINGi10116.ENSRNOP00000013919.

PTM databases

iPTMnetiP0C0S7.
PhosphoSiteiP0C0S7.

Proteomic databases

PaxDbiP0C0S7.
PRIDEiP0C0S7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi58940.
KEGGirno:58940.

Organism-specific databases

CTDi3015.
RGDi621464. H2afz.

Phylogenomic databases

eggNOGiKOG1757. Eukaryota.
COG5262. LUCA.
HOGENOMiHOG000234652.
HOVERGENiHBG009342.
InParanoidiP0C0S7.
KOiK11251.
PhylomeDBiP0C0S7.
TreeFamiTF354232.

Miscellaneous databases

PROiP0C0S7.

Gene expression databases

BgeeiENSRNOG00000010306.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR002119. Histone_H2A.
IPR007125. Histone_H2A/H2B/H3.
IPR032454. Histone_H2A_C.
IPR032458. Histone_H2A_CS.
[Graphical view]
PfamiPF00125. Histone. 1 hit.
PF16211. Histone_H2A_C. 1 hit.
[Graphical view]
PRINTSiPR00620. HISTONEH2A.
SMARTiSM00414. H2A. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00046. HISTONE_H2A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiH2AZ_RAT
AccessioniPrimary (citable) accession number: P0C0S7
Secondary accession number(s): P17317, Q5U5H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.