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Protein

Histone H2A.Z

Gene

H2afz

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. Essential for early development.3 Publications

Miscellaneous

Down-regulation of H2afz by RNA interference leads to death at early embryonic stages.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-1221632. Meiotic synapsis.
R-MMU-1221633. Meiotic Synapsis.
R-MMU-427389. ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression.
R-MMU-427413. NoRC negatively regulates rRNA expression.
R-MMU-5334118. DNA methylation.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-MMU-573389. NoRC negatively regulates rRNA expression.
R-MMU-912497. Meiotic Recombination.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2A.Z
Short name:
H2A/z
Gene namesi
Name:H2afz
Synonyms:H2az
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1888388. H2afz.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000552981 – 128Histone H2A.ZAdd BLAST128

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5N6-acetyllysine; alternate1 Publication1
Modified residuei5N6-methyllysine; alternateBy similarity1
Modified residuei8N6-acetyllysine; alternate1 Publication1
Modified residuei8N6-methyllysine; alternateBy similarity1
Modified residuei12N6-acetyllysine1 Publication1
Modified residuei14N6-acetyllysineBy similarity1
Cross-linki122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.By similarity
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.2 Publications
Not phosphorylated.1 Publication
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

EPDiP0C0S6.
MaxQBiP0C0S6.
PaxDbiP0C0S6.
PRIDEiP0C0S6.
TopDownProteomicsiP0C0S6.

PTM databases

iPTMnetiP0C0S6.
PhosphoSitePlusiP0C0S6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000037894.
CleanExiMM_H2AFZ.
ExpressionAtlasiP0C0S6. baseline and differential.
GenevisibleiP0C0S6. MM.

Interactioni

Subunit structurei

Interacts (via M6 cassette) with ANP32E; leading to removal of H2A.Z/H2AFZ from the nucleosome (By similarity). The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms a heterodimer with H2B (By similarity). H2AFZ interacts with INCENP (PubMed:12660166). Interacts with VPS72 (via N-terminal domain). Interacts with PWWP2A (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206173. 5 interactors.
IntActiP0C0S6. 13 interactors.
MINTiMINT-1605699.
STRINGi10090.ENSMUSP00000036907.

Structurei

3D structure databases

ProteinModelPortaliP0C0S6.
SMRiP0C0S6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 17Required for interaction with INCENP1 PublicationAdd BLAST17
Regioni89 – 100M6 cassetteBy similarityAdd BLAST12
Regioni93 – 103Required for interaction with INCENP1 PublicationAdd BLAST11
Regioni120 – 128Required for interaction with PWWP2ABy similarity9

Sequence similaritiesi

Belongs to the histone H2A family.Curated

Phylogenomic databases

eggNOGiKOG1757. Eukaryota.
COG5262. LUCA.
GeneTreeiENSGT00890000139387.
HOGENOMiHOG000234652.
HOVERGENiHBG009342.
InParanoidiP0C0S6.
KOiK11251.
OMAiXFPVGRI.
OrthoDBiEOG091G0XGD.
PhylomeDBiP0C0S6.
TreeFamiTF354232.

Family and domain databases

InterProiView protein in InterPro
IPR009072. Histone-fold.
IPR002119. Histone_H2A.
IPR007125. Histone_H2A/H2B/H3.
IPR032454. Histone_H2A_C.
IPR032458. Histone_H2A_CS.
PfamiView protein in Pfam
PF00125. Histone. 1 hit.
PF16211. Histone_H2A_C. 1 hit.
PRINTSiPR00620. HISTONEH2A.
SMARTiView protein in SMART
SM00414. H2A. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiView protein in PROSITE
PS00046. HISTONE_H2A. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C0S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGGKAGKDS GKAKTKAVSR SQRAGLQFPV GRIHRHLKSR TTSHGRVGAT
60 70 80 90 100
AAVYSAAILE YLTAEVLELA GNASKDLKVK RITPRHLQLA IRGDEELDSL
110 120
IKATIAGGGV IPHIHKSLIG KKGQQKTV
Length:128
Mass (Da):13,553
Last modified:January 23, 2007 - v2
Checksum:iE024E53818230371
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12K → E in BAE30460 (PubMed:16141072).Curated1
Sequence conflicti29P → A in BAE26959 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70494 mRNA. Translation: AAB09578.1.
AY074806 mRNA. Translation: AAL71864.1.
AK028176 mRNA. Translation: BAC25791.1.
AK088704 mRNA. Translation: BAC40515.1.
AK143572 mRNA. Translation: BAE25443.1.
AK146181 mRNA. Translation: BAE26959.1.
AK150454 mRNA. Translation: BAE29574.1.
AK151052 mRNA. Translation: BAE30069.1.
AK151509 mRNA. Translation: BAE30460.1.
AK151868 mRNA. Translation: BAE30756.1.
AK152398 mRNA. Translation: BAE31186.1.
AK152848 mRNA. Translation: BAE31541.1.
AK168178 mRNA. Translation: BAE40138.1.
BC079903 mRNA. Translation: AAH79903.1.
BC147478 mRNA. Translation: AAI47479.1.
BC158035 mRNA. Translation: AAI58036.1.
BC171999 mRNA. Translation: AAI71999.1.
CCDSiCCDS38647.1.
RefSeqiNP_058030.1. NM_016750.3.
UniGeneiMm.117541.
Mm.372513.
Mm.465508.

Genome annotation databases

EnsembliENSMUST00000041045; ENSMUSP00000036907; ENSMUSG00000037894.
GeneIDi51788.
KEGGimmu:51788.
UCSCiuc008rmo.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiH2AZ_MOUSE
AccessioniPrimary (citable) accession number: P0C0S6
Secondary accession number(s): B2RXZ3
, P17317, Q3UA55, Q3UK43, Q68FD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families