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Protein

Small-conductance mechanosensitive channel

Gene

mscS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell, opening in response to stretch forces in the membrane lipid bilayer, without the need for other proteins. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage; as the membrane is depolarized, less tension is required to open the channel and vice versa. The channel is characterized by short bursts of activity that last for a few seconds.
The channel pore is formed by TM3 and the loop between TM2 and TM3. After a sharp turn at Gly-113, an alpha-helix (residues 114-127) is oriented nearly parallel to the plane of the putative lipid bilayer. On the intracellular side of the channel, the permeation pathway of MscS does not connect directly to the cytoplasm but instead opens to a large chamber that is connected to the cytoplasm. This chamber resembles a molecular filter that could serve to prescreen large molecules before they are allowed passage to the transmembrane pore. The TM1 and TM2 helices appear to be likely candidates for mediating the tension and voltage sensitivities of MscS. Gating requires large rearrangements of at least the C-terminus.

GO - Molecular functioni

  • mechanically-gated ion channel activity Source: EcoCyc

GO - Biological processi

  • cellular water homeostasis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11160-MONOMER.
ECOL316407:JW2891-MONOMER.

Protein family/group databases

TCDBi1.A.23.2.1. the small conductance mechanosensitive ion channel (mscs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Small-conductance mechanosensitive channel
Gene namesi
Name:mscS
Synonyms:yggB
Ordered Locus Names:b2924, JW2891
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11160. mscS.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 28Periplasmic1 PublicationAdd BLAST28
Transmembranei29 – 57HelicalAdd BLAST29
Topological domaini58 – 67Cytoplasmic1 Publication10
Transmembranei68 – 91HelicalAdd BLAST24
Topological domaini92 – 95Periplasmic1 Publication4
Transmembranei96 – 113HelicalAdd BLAST18
Topological domaini114 – 286Cytoplasmic1 PublicationAdd BLAST173

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40V → C, G or N: No detectable phenotype. 1 Publication1
Mutagenesisi40V → D or K: Normal growth stops, without cell death, due to increased membrane permeability to potassium ions and protons (permeability tested only for D substitutions). 1 Publication1
Mutagenesisi58S → C: Readily forms disulfide bonds with cross-linkers, suggesting that individual S-58 are only 3 Angstroms apart in the closed state, versus 33 Angstroms apart in the open state crystal structure. 1 Publication1
Mutagenesisi266 – 286ISFPY…EDKAA → HHHHHHLE: Normal levels of channels are expressed; they recover more slowly than wild-type cells after desensitization. 1 PublicationAdd BLAST21
Mutagenesisi266 – 286ISFPY…EDKAA → LE: Fewer channels present in the membrane, they require slightly more pressure to open and do not recover after desensitization. 1 PublicationAdd BLAST21
Mutagenesisi267S → C: Provides biochemical evidence for heptameric structure upon cross-linking. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001102381 – 286Small-conductance mechanosensitive channelAdd BLAST286

Proteomic databases

PaxDbiP0C0S1.
PRIDEiP0C0S1.

Interactioni

Subunit structurei

Homoheptamer.3 Publications

Protein-protein interaction databases

BioGridi4259238. 239 interactors.
DIPiDIP-36192N.
IntActiP0C0S1. 2 interactors.
STRINGi511145.b2924.

Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 58Combined sources37
Helixi63 – 87Combined sources25
Turni88 – 90Combined sources3
Helixi93 – 127Combined sources35
Beta strandi135 – 140Combined sources6
Beta strandi142 – 148Combined sources7
Beta strandi150 – 156Combined sources7
Beta strandi162 – 166Combined sources5
Helixi167 – 172Combined sources6
Beta strandi175 – 192Combined sources18
Helixi198 – 211Combined sources14
Beta strandi221 – 228Combined sources8
Beta strandi233 – 242Combined sources10
Turni243 – 245Combined sources3
Helixi246 – 264Combined sources19
Beta strandi272 – 279Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OAUX-ray3.70A/B/C/D/E/F/G1-286[»]
2VV5X-ray3.45A/B/C/D/E/F/G1-286[»]
3UDCX-ray3.36A/B/C/D/E/F/G272-286[»]
4AGEX-ray4.84A/B/C/D/E/F/G1-286[»]
4AGFX-ray4.70A/B/C/D/E/F/G1-286[»]
4HWAX-ray4.37A/B/C/D/E/F/G1-286[»]
5AJIX-ray2.99A/B/C/D/E/F/G1-286[»]
ProteinModelPortaliP0C0S1.
SMRiP0C0S1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0S1.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D64. Bacteria.
COG0668. LUCA.
HOGENOMiHOG000110050.
InParanoidiP0C0S1.
KOiK03442.
OMAiWPVYWDL.
PhylomeDBiP0C0S1.

Family and domain databases

InterProiIPR010920. LSM_dom.
IPR011066. MscC_channel_C.
IPR006685. MscS_channel.
IPR006686. MscS_channel_CS.
IPR011014. MscS_channel_TM-2.
IPR008910. TM_helix.
[Graphical view]
PfamiPF00924. MS_channel. 1 hit.
PF05552. TM_helix. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
SSF82689. SSF82689. 1 hit.
SSF82861. SSF82861. 1 hit.
PROSITEiPS01246. UPF0003. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C0S1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDLNVVDSI NGAGSWLVAN QALLLSYAVN IVAALAIIIV GLIIARMISN
60 70 80 90 100
AVNRLMISRK IDATVADFLS ALVRYGIIAF TLIAALGRVG VQTASVIAVL
110 120 130 140 150
GAAGLAVGLA LQGSLSNLAA GVLLVMFRPF RAGEYVDLGG VAGTVLSVQI
160 170 180 190 200
FSTTMRTADG KIIVIPNGKI IAGNIINFSR EPVRRNEFII GVAYDSDIDQ
210 220 230 240 250
VKQILTNIIQ SEDRILKDRE MTVRLNELGA SSINFVVRVW SNSGDLQNVY
260 270 280
WDVLERIKRE FDAAGISFPY PQMDVNFKRV KEDKAA
Length:286
Mass (Da):30,896
Last modified:December 6, 2005 - v1
Checksum:iFF00AD64F795E9FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14436 Genomic DNA. Translation: CAA32606.1.
U28377 Genomic DNA. Translation: AAA69091.1.
U00096 Genomic DNA. Translation: AAC75961.1.
AP009048 Genomic DNA. Translation: BAE76988.1.
PIRiS04735. QQEC4A.
RefSeqiNP_417399.1. NC_000913.3.
WP_000389818.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75961; AAC75961; b2924.
BAE76988; BAE76988; BAE76988.
GeneIDi947416.
KEGGiecj:JW2891.
eco:b2924.
PATRICi32121262. VBIEscCol129921_3019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14436 Genomic DNA. Translation: CAA32606.1.
U28377 Genomic DNA. Translation: AAA69091.1.
U00096 Genomic DNA. Translation: AAC75961.1.
AP009048 Genomic DNA. Translation: BAE76988.1.
PIRiS04735. QQEC4A.
RefSeqiNP_417399.1. NC_000913.3.
WP_000389818.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OAUX-ray3.70A/B/C/D/E/F/G1-286[»]
2VV5X-ray3.45A/B/C/D/E/F/G1-286[»]
3UDCX-ray3.36A/B/C/D/E/F/G272-286[»]
4AGEX-ray4.84A/B/C/D/E/F/G1-286[»]
4AGFX-ray4.70A/B/C/D/E/F/G1-286[»]
4HWAX-ray4.37A/B/C/D/E/F/G1-286[»]
5AJIX-ray2.99A/B/C/D/E/F/G1-286[»]
ProteinModelPortaliP0C0S1.
SMRiP0C0S1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259238. 239 interactors.
DIPiDIP-36192N.
IntActiP0C0S1. 2 interactors.
STRINGi511145.b2924.

Protein family/group databases

TCDBi1.A.23.2.1. the small conductance mechanosensitive ion channel (mscs) family.

Proteomic databases

PaxDbiP0C0S1.
PRIDEiP0C0S1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75961; AAC75961; b2924.
BAE76988; BAE76988; BAE76988.
GeneIDi947416.
KEGGiecj:JW2891.
eco:b2924.
PATRICi32121262. VBIEscCol129921_3019.

Organism-specific databases

EchoBASEiEB1149.
EcoGeneiEG11160. mscS.

Phylogenomic databases

eggNOGiENOG4105D64. Bacteria.
COG0668. LUCA.
HOGENOMiHOG000110050.
InParanoidiP0C0S1.
KOiK03442.
OMAiWPVYWDL.
PhylomeDBiP0C0S1.

Enzyme and pathway databases

BioCyciEcoCyc:EG11160-MONOMER.
ECOL316407:JW2891-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0C0S1.
PROiP0C0S1.

Family and domain databases

InterProiIPR010920. LSM_dom.
IPR011066. MscC_channel_C.
IPR006685. MscS_channel.
IPR006686. MscS_channel_CS.
IPR011014. MscS_channel_TM-2.
IPR008910. TM_helix.
[Graphical view]
PfamiPF00924. MS_channel. 1 hit.
PF05552. TM_helix. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
SSF82689. SSF82689. 1 hit.
SSF82861. SSF82861. 1 hit.
PROSITEiPS01246. UPF0003. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSCS_ECOLI
AccessioniPrimary (citable) accession number: P0C0S1
Secondary accession number(s): P11666, Q2M9R8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.