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P0C0S1

- MSCS_ECOLI

UniProt

P0C0S1 - MSCS_ECOLI

Protein

Small-conductance mechanosensitive channel

Gene

mscS

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell, opening in response to stretch forces in the membrane lipid bilayer, without the need for other proteins. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage; as the membrane is depolarized, less tension is required to open the channel and vice versa. The channel is characterized by short bursts of activity that last for a few seconds.
    The channel pore is formed by TM3 and the loop between TM2 and TM3. After a sharp turn at Gly-113, an alpha-helix (residues 114-127) is oriented nearly parallel to the plane of the putative lipid bilayer. On the intracellular side of the channel, the permeation pathway of MscS does not connect directly to the cytoplasm but instead opens to a large chamber that is connected to the cytoplasm. This chamber resembles a molecular filter that could serve to prescreen large molecules before they are allowed passage to the transmembrane pore. The TM1 and TM2 helices appear to be likely candidates for mediating the tension and voltage sensitivities of MscS. Gating requires large rearrangements of at least the C-terminus.

    GO - Molecular functioni

    1. mechanically-gated ion channel activity Source: EcoCyc

    GO - Biological processi

    1. cellular water homeostasis Source: EcoCyc
    2. ion transmembrane transport Source: GOC

    Keywords - Molecular functioni

    Ion channel

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11160-MONOMER.
    ECOL316407:JW2891-MONOMER.
    RETL1328306-WGS:GSTH-1410-MONOMER.

    Protein family/group databases

    TCDBi1.A.23.2.1. the small conductance mechanosensitive ion channel (mscs) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Small-conductance mechanosensitive channel
    Gene namesi
    Name:mscS
    Synonyms:yggB
    Ordered Locus Names:b2924, JW2891
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11160. mscS.

    Subcellular locationi

    Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: EcoCyc
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi40 – 401V → C, G or N: No detectable phenotype. 2 Publications
    Mutagenesisi40 – 401V → D or K: Normal growth stops, without cell death, due to increased membrane permeability to potassium ions and protons (permeability tested only for D substitutions). 2 Publications
    Mutagenesisi58 – 581S → C: Readily forms disulfide bonds with cross-linkers, suggesting that individual S-58 are only 3 Angstroms apart in the closed state, versus 33 Angstroms apart in the open state crystal structure. 2 Publications
    Mutagenesisi266 – 28621ISFPY…EDKAA → HHHHHHLE: Normal levels of channels are expressed; they recover more slowly than wild-type cells after desensitization. 1 PublicationAdd
    BLAST
    Mutagenesisi266 – 28621ISFPY…EDKAA → LE: Fewer channels present in the membrane, they require slightly more pressure to open and do not recover after desensitization. 1 PublicationAdd
    BLAST
    Mutagenesisi267 – 2671S → C: Provides biochemical evidence for heptameric structure upon cross-linking. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 286286Small-conductance mechanosensitive channelPRO_0000110238Add
    BLAST

    Proteomic databases

    PaxDbiP0C0S1.
    PRIDEiP0C0S1.

    Expressioni

    Gene expression databases

    GenevestigatoriP0C0S1.

    Interactioni

    Subunit structurei

    Homoheptamer.3 Publications

    Protein-protein interaction databases

    DIPiDIP-36192N.
    IntActiP0C0S1. 2 interactions.
    STRINGi511145.b2924.

    Structurei

    Secondary structure

    1
    286
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi26 – 283
    Helixi30 – 5930
    Helixi63 – 8725
    Turni88 – 903
    Helixi96 – 11116
    Helixi113 – 12513
    Beta strandi135 – 1406
    Beta strandi142 – 1487
    Beta strandi150 – 1567
    Beta strandi162 – 1665
    Helixi167 – 1715
    Beta strandi175 – 19218
    Helixi198 – 21114
    Beta strandi221 – 2288
    Beta strandi230 – 24213
    Turni243 – 2453
    Helixi246 – 26419
    Beta strandi274 – 2796

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2OAUX-ray3.70A/B/C/D/E/F/G1-286[»]
    2VV5X-ray3.45A/B/C/D/E/F/G1-286[»]
    3UDCX-ray3.36A/B/C/D/E/F/G272-286[»]
    4AGEX-ray4.84A/B/C/D/E/F/G1-286[»]
    4AGFX-ray4.70A/B/C/D/E/F/G1-286[»]
    4HWAX-ray4.37A/B/C/D/E/F/G1-286[»]
    ProteinModelPortaliP0C0S1.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0C0S1.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2828Periplasmic1 PublicationAdd
    BLAST
    Topological domaini58 – 6710Cytoplasmic1 Publication
    Topological domaini92 – 954Periplasmic1 Publication
    Topological domaini114 – 286173Cytoplasmic1 PublicationAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei29 – 5729HelicalAdd
    BLAST
    Transmembranei68 – 9124HelicalAdd
    BLAST
    Transmembranei96 – 11318HelicalAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0668.
    HOGENOMiHOG000110050.
    KOiK03442.
    OMAiILYISEY.
    OrthoDBiEOG61GG69.
    PhylomeDBiP0C0S1.

    Family and domain databases

    InterProiIPR010920. LSM_dom.
    IPR011066. MscC_channel_C.
    IPR006685. MscS_channel.
    IPR006686. MscS_channel_CS.
    IPR011014. MscS_channel_TM-2.
    IPR008910. TM_helix.
    [Graphical view]
    PfamiPF00924. MS_channel. 1 hit.
    PF05552. TM_helix. 1 hit.
    [Graphical view]
    SUPFAMiSSF50182. SSF50182. 1 hit.
    SSF82689. SSF82689. 1 hit.
    SSF82861. SSF82861. 1 hit.
    PROSITEiPS01246. UPF0003. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0C0S1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDLNVVDSI NGAGSWLVAN QALLLSYAVN IVAALAIIIV GLIIARMISN    50
    AVNRLMISRK IDATVADFLS ALVRYGIIAF TLIAALGRVG VQTASVIAVL 100
    GAAGLAVGLA LQGSLSNLAA GVLLVMFRPF RAGEYVDLGG VAGTVLSVQI 150
    FSTTMRTADG KIIVIPNGKI IAGNIINFSR EPVRRNEFII GVAYDSDIDQ 200
    VKQILTNIIQ SEDRILKDRE MTVRLNELGA SSINFVVRVW SNSGDLQNVY 250
    WDVLERIKRE FDAAGISFPY PQMDVNFKRV KEDKAA 286
    Length:286
    Mass (Da):30,896
    Last modified:December 6, 2005 - v1
    Checksum:iFF00AD64F795E9FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14436 Genomic DNA. Translation: CAA32606.1.
    U28377 Genomic DNA. Translation: AAA69091.1.
    U00096 Genomic DNA. Translation: AAC75961.1.
    AP009048 Genomic DNA. Translation: BAE76988.1.
    PIRiS04735. QQEC4A.
    RefSeqiNP_417399.1. NC_000913.3.
    YP_491124.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75961; AAC75961; b2924.
    BAE76988; BAE76988; BAE76988.
    GeneIDi12930244.
    947416.
    KEGGiecj:Y75_p2855.
    eco:b2924.
    PATRICi32121262. VBIEscCol129921_3019.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14436 Genomic DNA. Translation: CAA32606.1 .
    U28377 Genomic DNA. Translation: AAA69091.1 .
    U00096 Genomic DNA. Translation: AAC75961.1 .
    AP009048 Genomic DNA. Translation: BAE76988.1 .
    PIRi S04735. QQEC4A.
    RefSeqi NP_417399.1. NC_000913.3.
    YP_491124.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2OAU X-ray 3.70 A/B/C/D/E/F/G 1-286 [» ]
    2VV5 X-ray 3.45 A/B/C/D/E/F/G 1-286 [» ]
    3UDC X-ray 3.36 A/B/C/D/E/F/G 272-286 [» ]
    4AGE X-ray 4.84 A/B/C/D/E/F/G 1-286 [» ]
    4AGF X-ray 4.70 A/B/C/D/E/F/G 1-286 [» ]
    4HWA X-ray 4.37 A/B/C/D/E/F/G 1-286 [» ]
    ProteinModelPortali P0C0S1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-36192N.
    IntActi P0C0S1. 2 interactions.
    STRINGi 511145.b2924.

    Protein family/group databases

    TCDBi 1.A.23.2.1. the small conductance mechanosensitive ion channel (mscs) family.

    Proteomic databases

    PaxDbi P0C0S1.
    PRIDEi P0C0S1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75961 ; AAC75961 ; b2924 .
    BAE76988 ; BAE76988 ; BAE76988 .
    GeneIDi 12930244.
    947416.
    KEGGi ecj:Y75_p2855.
    eco:b2924.
    PATRICi 32121262. VBIEscCol129921_3019.

    Organism-specific databases

    EchoBASEi EB1149.
    EcoGenei EG11160. mscS.

    Phylogenomic databases

    eggNOGi COG0668.
    HOGENOMi HOG000110050.
    KOi K03442.
    OMAi ILYISEY.
    OrthoDBi EOG61GG69.
    PhylomeDBi P0C0S1.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11160-MONOMER.
    ECOL316407:JW2891-MONOMER.
    RETL1328306-WGS:GSTH-1410-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0C0S1.
    PROi P0C0S1.

    Gene expression databases

    Genevestigatori P0C0S1.

    Family and domain databases

    InterProi IPR010920. LSM_dom.
    IPR011066. MscC_channel_C.
    IPR006685. MscS_channel.
    IPR006686. MscS_channel_CS.
    IPR011014. MscS_channel_TM-2.
    IPR008910. TM_helix.
    [Graphical view ]
    Pfami PF00924. MS_channel. 1 hit.
    PF05552. TM_helix. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50182. SSF50182. 1 hit.
    SSF82689. SSF82689. 1 hit.
    SSF82861. SSF82861. 1 hit.
    PROSITEi PS01246. UPF0003. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification, molecular cloning and sequence analysis of a gene cluster encoding the class II fructose 1,6-bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli."
      Alefounder P.R., Perham R.N.
      Mol. Microbiol. 3:723-732(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / CS520.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: CHARACTERIZATION.
      Strain: K12 / AW405.
    5. "Characterization of mechanosensitive channels in Escherichia coli cytoplasmic membrane by whole-cell patch clamp recording."
      Cui C., Smith D.O., Adler J.
      J. Membr. Biol. 144:31-42(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: SENSITIVITY TO OSMOTIC AND VOLTAGE DIFFERENCES ACROSS THE CYTOPLASMIC MEMBRANE.
      Strain: K12.
    6. "Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity."
      Levina N., Toetemeyer S., Stokes N.R., Louis P., Jones M.A., Booth I.R.
      EMBO J. 18:1730-1737(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, PHYIOLOGICAL ROLE.
      Strain: K12 / MJF379.
    7. "Purification of the small mechanosensitive channel of Escherichia coli (MscS): the subunit structure, conduction, and gating characteristics in liposomes."
      Sukharev S.
      Biophys. J. 83:290-298(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: LIPOSOME RECONSTITUTION.
      Strain: K12 / AW405.
    8. "Functional design of bacterial mechanosensitive channels. Comparisons and contrasts illuminated by random mutagenesis."
      Okada K., Moe P.C., Blount P.
      J. Biol. Chem. 277:27682-27688(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: LIPOSOME RECONSTITUTION, MUTAGENESIS OF VAL-40.
      Strain: K12 / MJF379.
    9. "C termini of the Escherichia coli mechanosensitive ion channel (MscS) move apart upon the channel opening."
      Koprowski P., Kubalski A.
      J. Biol. Chem. 278:11237-11245(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CROSS-LINKING OF THE C-TERMINI INHIBITS CHANNEL OPENING.
      Strain: K12 / MJF379.
    10. "The closed structure of the MscS mechanosensitive channel. Cross-linking of single cysteine mutants."
      Miller S., Edwards M.D., Ozdemir C., Booth I.R.
      J. Biol. Chem. 278:32246-32250(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT IN THE CLOSED STATE, MUTAGENESIS OF SER-58 AND SER-267.
    11. "The conserved carboxy-terminus of the MscS mechanosensitive channel is not essential but increases stability and activity."
      Schumann U., Edwards M.D., Li C., Booth I.R.
      FEBS Lett. 572:233-237(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: C-TERMINAL DELETIONS.
    12. Cited for: SUBUNIT, SUBCELLULAR LOCATION.
      Strain: BL21-DE3.
    13. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.
    14. "Pivotal role of the glycine-rich TM3 helix in gating the MscS mechanosensitive channel."
      Edwards M.D., Li Y., Kim S., Miller S., Bartlett W., Black S., Dennison S., Iscla I., Blount P., Bowie J.U., Booth I.R.
      Nat. Struct. Mol. Biol. 12:113-119(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF CONSERVED GLY AND ALA RESIDUES IN TM3, PRESENTATION OF MODELS OF THE CLOSED STATE CHANNEL AND CLOSED TO OPEN STATE TRANSITIONS.
      Strain: K12 / MJF431.
    15. "Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel."
      Bass R.B., Strop P., Barclay M., Rees D.C.
      Science 298:1582-1587(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.9 ANGSTROMS) OF 27-280 PROBABLY IN THE OPEN STATE, SUBUNIT.
      Strain: K12.

    Entry informationi

    Entry nameiMSCS_ECOLI
    AccessioniPrimary (citable) accession number: P0C0S1
    Secondary accession number(s): P11666, Q2M9R8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3