Reviewed, UniProtKB/Swiss-Prot P0C0R0 (GYRA_STAES)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	SE_0005

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	893 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 893	893	DNA gyrase subunit A		PRO_0000145259
Sites
	Active site	123	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C0R0-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 2A6A7CD345A526CE	FASTA	893	100,114
	10 20 30 40 50 60 MAELPQSRIN ERNITSEMRE SFLDYAMSVI VSRALPDVRD GLKPVHRRIL YGLNEQGMTP 70 80 90 100 110 120 DKPYKKSARI VGDVMGKYHP HGDSSIYEAM VRMAQDFSYR YPLVDGQGNF GSMDGDGAAA 130 140 150 160 170 180 MRYTEARMTK ITLELLRDIN KDTIDFIDNY DGNEREPSVL PARFPNLLVN GAAGIAVGMA 190 200 210 220 230 240 TNIPPHNLTE VIDGVLSLSK NPDITINELM EDIQGPDFPT AGLVLGKSGI RRAYETGRGS 250 260 270 280 290 300 IQMRSRAEIE ERGGGRQRIV VTEIPFQVNK ARMIEKIAEL VRDKKIDGIT DLRDETSLRT 310 320 330 340 350 360 GVRVVIDVRK DANASVILNN LYKQTPLQTS FGVNMIALVN GRPKLINLKE ALIHYLEHQK 370 380 390 400 410 420 TVVRRRTEYN LKKARDRAHI LEGLRIALDH IDEIITTIRE SDTDKIAMAS LQERFKLTER 430 440 450 460 470 480 QAQAILDMRL RRLTGLERDK IESEYNELLE YIKELEEILA DEEVLLQLVR DELTEIKERF 490 500 510 520 530 540 GDERRTEIQL GGLEDLEDED LIPEEQIVIT LSHNNYIKRL PVSTYRSQNR GGRGIQGMNT 550 560 570 580 590 600 LDEDFVSQLV TMSTHDHVLF FTNKGRVYKL KGYEVPELSR QSKGIPIINA IELENDETIS 610 620 630 640 650 660 TMIAVKDLES EEDYLVFATK QGIVKRSSLS NFSRINKNGK IAINFKEDDE LIAVRLTTGN 670 680 690 700 710 720 EDILIGTAHA SLIRFSESTL RPLGRTAAGV KGISLREGDT VVGLDVADSE SEDEVLVVTE 730 740 750 760 770 780 NGYGKRTPVS EYRLSNRGGK GIKTATITER NGNIVCITTV TGEEDLMVVT NAGVIIRLDV 790 800 810 820 830 840 HDISQNGRAA QGVRLMKLGD GQFVSTVAKV NEEDDNEENA DEAQQSTTTE TADVEEVVDD 850 860 870 880 890 QTPGNAIHTE GDAEMESVES PENDDRIDIR QDFMDRVNED IESASDNEED SDE

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO03602.1.
RefSeq	NP_763560.1.
3D structure databases
SMR	P0C0R0. Positions 31-489, 504-812.
ModBase	Search...
Protein-protein interaction databases
STRING	P0C0R0.
Genome annotation databases
GeneID	1056827.
GenomeReviews	Gene locus SE_0005 in contig AE015929_GR.
KEGG	sep:SE0005.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0188.
HOGENOM	HBG635183.
OMA	ELGNDWN.
PhylomeDB	P0C0R0.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_0005-MONOMER.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_STAES

Accession

Primary (citable) accession number: P0C0R0
Secondary accession number(s): P54112

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)