Reviewed, UniProtKB/Swiss-Prot P0C0Q9 (GYRA_STAEP)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:

gyrA

Organism

Staphylococcus epidermidis

Taxonomic identifier

1282 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	94 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›94	›94	DNA gyrase subunit A		PRO_0000145258
Experimental info
	Mutagenesis	84	1	S → F: Resistant to ciprofloxacin. Ref.1
	Non-terminal residue	94	1

Sequences

	Sequence		Length	Mass (Da)
	P0C0Q9-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 72862CF17C045728	FASTA	94	10,723
	10 20 30 40 50 60 MAELPQSRIN ERNITSEMRE SFLDYAMSVI VSRALPDVRD GLKPVHRRIL YGLNEQGMTP 70 80 90 DKPYKKSARI VGDVMGKYHP HGDSSIYEAM VRMA

References

[1]

"Ciprofloxacin resistance in coagulase-positive and -negative staphylococci: role of mutations at serine 84 in the DNA gyrase A protein of Staphylococcus aureus and Staphylococcus epidermidis." Sreedharan S., Peterson L.R., Fisher L.M. Antimicrob. Agents Chemother. 35:2151-2154(1991) [PubMed: 1662027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF SER-84.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S72603 Genomic DNA. Translation: AAB20672.1.
PIR	A49832.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
SMR	P0C0Q9. Positions 31-94.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.99.1.3. 874.
Family and domain databases
InterPro	IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit.
Pfam	PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

GYRA_STAEP

Accession

Primary (citable) accession number: P0C0Q9
Secondary accession number(s): P54112

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)