Reviewed, UniProtKB/Swiss-Prot P0C0Q2 (GSEA_STAES)

Names and origin

Protein names

Recommended name:
    Glutamyl endopeptidase
    EC=3.4.21.19
Alternative name(s):
    Glutamic acid-specific protease
      Short name=GluSE

Gene names

Name:	gseA
Synonyms:	esp
Ordered Locus Names:	SE_1543

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exhibits a significant hydrolytic activity for the carbonyl side of glutamic acid. Shows activity toward human fibronectin and type 1 collagen By similarity.
Catalytic activity	Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the peptidase S1B family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Propeptide	28 – 66	39	By similarity		PRO_0000042921
	Chain	67 – 282	216	Glutamyl endopeptidase		PRO_0000042922
Sites
	Active site	117	1	Charge relay system By similarity
	Active site	159	1	Charge relay system By similarity
	Active site	235	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C0Q2-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 5DAD3E8D7EB3E0CB	FASTA	282	30,809
	10 20 30 40 50 60 MKKRFLSICT MTIAALATTT MVNTSYAKTD TESHNHSSLG TENKNVLDIN SSSHNIKPSQ 70 80 90 100 110 120 NKSYPSVILP NNNRHQIFNT TQGHYDAVSF IYIPIDGGYM SGSGVVVGEN EILTNKHVVN 130 140 150 160 170 180 GAKGNPRNIS VHPSAKNEND YPNGKFVGQE IIPYPGNSDL AILRVSPNEH NQHIGQVVKP 190 200 210 220 230 240 ATISSNTDTR INENITVTGY PGDKPLATMW ESVGKVVYIG GEELRYDLST VGGNSGSPVF 250 260 270 280 NGKNQVIGIH YGGVDNKYNS SVYINDFVQQ FLRNNIPDIN IQ

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015929 Genomic DNA. Translation: AAO05142.1.
RefSeq	NP_765098.1.
3D structure databases
HSSP	HSSP built from PDB template 1DT2 based on UniProtKB P09332.
ModBase	Search...
Protein family/group databases
MEROPS	S01.269.
Genome annotation databases
GeneID	1055780.
GenomeReviews	Gene locus SE_1543 in contig AE015929_GR.
KEGG	sep:SE1543.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0C0Q2.
OMA	P0C0Q2. EDINFAN.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_1543-MON.
Family and domain databases
InterPro	IPR000126. Pept_S1B_AS. IPR001254. Peptidase_S1_S6. IPR008256. Peptidase_S1B. IPR008353. Peptidase_S1B_tx. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR01774. EXFOLTOXIN. PR00839. V8PROTEASE.
PROSITE	PS00672. V8_HIS. False negative. PS00673. V8_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSEA_STAES

Accession

Primary (citable) accession number: P0C0Q2
Secondary accession number(s): Q7DG19, Q8CMC1, Q9AJX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)