P0C0Q2 (GSEA_STAES) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl endopeptidase
EC=3.4.21.19

Alternative name(s):
Glutamic acid-specific protease
Short name=GluSE

Gene names

Name:	gseA
Synonyms:	esp
Ordered Locus Names:	SE_1543

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus epidermidis

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exhibits a significant hydrolytic activity for the carbonyl side of glutamic acid. Shows activity toward human fibronectin and type 1 collagen By similarity.
Catalytic activity	Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the peptidase S1B family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Propeptide	28 – 66	39	By similarity		PRO_0000042921
	Chain	67 – 282	216	Glutamyl endopeptidase		PRO_0000042922
Sites
	Active site	117	1	Charge relay system By similarity
	Active site	159	1	Charge relay system By similarity
	Active site	235	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C0Q2 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 5DAD3E8D7EB3E0CB	FASTA	282	30,809
	10 20 30 40 50 60 MKKRFLSICT MTIAALATTT MVNTSYAKTD TESHNHSSLG TENKNVLDIN SSSHNIKPSQ 70 80 90 100 110 120 NKSYPSVILP NNNRHQIFNT TQGHYDAVSF IYIPIDGGYM SGSGVVVGEN EILTNKHVVN 130 140 150 160 170 180 GAKGNPRNIS VHPSAKNEND YPNGKFVGQE IIPYPGNSDL AILRVSPNEH NQHIGQVVKP 190 200 210 220 230 240 ATISSNTDTR INENITVTGY PGDKPLATMW ESVGKVVYIG GEELRYDLST VGGNSGSPVF 250 260 270 280 NGKNQVIGIH YGGVDNKYNS SVYINDFVQQ FLRNNIPDIN IQ

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO05142.1.
RefSeq	NP_765098.1. NC_004461.1.
3D structure databases
ProteinModelPortal	P0C0Q2.
SMR	P0C0Q2. Positions 67-281.
ModBase	Search...
Protein-protein interaction databases
STRING	176280.SE1543.
Protein family/group databases
MEROPS	S01.522.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO05142; AAO05142; SE_1543.
GeneID	1055780.
KEGG	sep:SE1543.
PATRIC	19608888. VBIStaEpi113981_1509.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3591.
KO	K01318.
OMA	IEDINFA.
ProtClustDB	CLSK885099.
Family and domain databases
InterPro	IPR000126. Pept_S1B_AS. IPR008256. Peptidase_S1B. IPR008353. Peptidase_S1B_tx. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
PRINTS	PR01774. EXFOLTOXIN. PR00839. V8PROTEASE.
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS00672. V8_HIS. False negative. PS00673. V8_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSEA_STAES

Accession

Primary (citable) accession number: P0C0Q2
Secondary accession number(s): Q7DG19, Q8CMC1, Q9AJX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program