Reviewed, UniProtKB/Swiss-Prot P0C0Q1 (GSEA_STAEP)

Names and origin

Protein names

Recommended name:
    Glutamyl endopeptidase
    EC=3.4.21.19
Alternative name(s):
    Glutamic acid-specific protease
      Short name=GluSE

Gene names

Name:	gseA
Synonyms:	esp

Organism

Staphylococcus epidermidis

Taxonomic identifier

1282 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Exhibits a significant hydrolytic activity for the carbonyl side of glutamic acid. Shows activity toward human fibronectin and type 1 collagen. Ref.1
Catalytic activity	Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa.
Enzyme regulation	Inhibited by diisopropyl fluorophosphate. Ref.1
Subunit structure	Monomer Probable. Ref.1
Subcellular location	Secreted Ref.1.
Sequence similarities	Belongs to the peptidase S1B family.
Biophysicochemical properties	pH dependence: Optimum pH is 8.0. Active from pH 6.0 to 9.0.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Propeptide	28 – 66	39			PRO_0000026896
	Chain	67 – 282	216	Glutamyl endopeptidase		PRO_0000026897
Sites
	Active site	117	1	Charge relay system By similarity
	Active site	159	1	Charge relay system By similarity
	Active site	235	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C0Q1-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 5DAD3E8D7EB3E0CB	FASTA	282	30,809
	10 20 30 40 50 60 MKKRFLSICT MTIAALATTT MVNTSYAKTD TESHNHSSLG TENKNVLDIN SSSHNIKPSQ 70 80 90 100 110 120 NKSYPSVILP NNNRHQIFNT TQGHYDAVSF IYIPIDGGYM SGSGVVVGEN EILTNKHVVN 130 140 150 160 170 180 GAKGNPRNIS VHPSAKNEND YPNGKFVGQE IIPYPGNSDL AILRVSPNEH NQHIGQVVKP 190 200 210 220 230 240 ATISSNTDTR INENITVTGY PGDKPLATMW ESVGKVVYIG GEELRYDLST VGGNSGSPVF 250 260 270 280 NGKNQVIGIH YGGVDNKYNS SVYINDFVQQ FLRNNIPDIN IQ

References

[1]

"Characterization and molecular cloning of a glutamyl endopeptidase from Staphylococcus epidermidis." Ohara-Nemoto Y., Ikeda Y., Kobayashi M., Sasaki M., Tajika S., Kimura S. Microb. Pathog. 33:33-41(2002) [PubMed: 12127798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 67-85, FUNCTION, ENZYME REGULATION, SUBUNIT, SUBCELLULAR LOCATION. Strain: ATCC 14990 / DSM 20044 / CIP 81.55.

[2]

"Molecular cloning and biochemical characterisation of proteases from Staphylcoccus epidermidis." Dubin G., Chmiel D., Mak P., Rakwalska M., Rzychon M., Dubin A. Biol. Chem. 382:1575-1582(2001) [PubMed: 11767947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 66-282, PROTEIN SEQUENCE OF 67-96. Strain: 6746.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB096695 Genomic DNA. Translation: BAC24763.1. AJ305145 Genomic DNA. Translation: CAC27157.1.
3D structure databases
SMR	P0C0Q1. Positions 67-281.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.21.19. 874.
Family and domain databases
InterPro	IPR000126. Pept_S1B_AS. IPR001254. Peptidase_S1_S6. IPR008256. Peptidase_S1B. IPR008353. Peptidase_S1B_tx. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR01774. EXFOLTOXIN. PR00839. V8PROTEASE.
PROSITE	PS00672. V8_HIS. False negative. PS00673. V8_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSEA_STAEP

Accession

Primary (citable) accession number: P0C0Q1
Secondary accession number(s): Q7DG19, Q8CMC1, Q9AJX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	January 19, 2010
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)