Reviewed, UniProtKB/Swiss-Prot P0C0P1 (DYR_STAES)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase
      Short name=DHFR
    EC=1.5.1.3

Gene names

Name:	folA
Synonyms:	dfrC, folA1
Ordered Locus Names:	SE_1119

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	Dihydrofolate reductase		PRO_0000186414
Regions
	Domain	2 – 157	156	DHFR
	Nucleotide binding	7 – 8	2	NADP By similarity
	Nucleotide binding	15 – 20	6	NADP By similarity
	Nucleotide binding	44 – 47	4	NADP By similarity
	Nucleotide binding	63 – 66	4	NADP By similarity
	Nucleotide binding	93 – 98	6	NADP By similarity
	Region	6 – 8	3	Substrate binding By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	58	1	Substrate By similarity
	Binding site	112	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C0P1-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: CB3167940A387EE0	FASTA	161	18,417
	10 20 30 40 50 60 MTLSIIVAHD KQRVIGYQNQ LPWHLPNDLK HVKQLTTGNT LVMGRKTFNS IGKPLPNRRN 70 80 90 100 110 120 VVLTNQASFH HEGVDVINSL DEIKELSGHV FIFGGQTLFE AMIDQVDDMY ITVIDGKFQG 130 140 150 160 DTFFPPYTFE NWEVESSVEG QLDEKNTIPH TFLHLVRRKG K

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO04716.1.
RefSeq	NP_764674.1.
3D structure databases
SMR	P0C0P1. Positions 3-158.
ModBase	Search...
Protein-protein interaction databases
STRING	P0C0P1.
Genome annotation databases
GeneID	1057990.
GenomeReviews	Gene locus SE_1119 in contig AE015929_GR.
KEGG	sep:SE1119.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0262.
HOGENOM	HBG665708.
OMA	GLPWHLP.
PhylomeDB	P0C0P1.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_1119-MONOMER.
Family and domain databases
InterPro	IPR012259. DHFR. IPR017925. Dihydrofolate_reductase_CS. IPR001796. Dihydrofolate_reductase_dom. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_STAES

Accession

Primary (citable) accession number: P0C0P1
Secondary accession number(s): Q59908

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)