ID   DYR_STAEP               Reviewed;         161 AA.
AC   P0C0P0; Q59908;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=Dihydrofolate reductase;
DE            Short=DHFR;
DE            EC=1.5.1.3;
GN   Name=folA; Synonyms=dfrC, folA1;
OS   Staphylococcus epidermidis.
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=1282;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS.
RC   STRAIN=ATCC 14990 / DSM 20044 / CIP 81.55;
RX   MEDLINE=95286471; PubMed=7768789;
RA   Dale G.E., Broger C., Hartman P.G., Langen H., Page M.G.P., Then R.L.,
RA   Stueber D.;
RT   "Characterization of the gene for the chromosomal dihydrofolate
RT   reductase (DHFR) of Staphylococcus epidermidis ATCC 14990: the origin
RT   of the trimethoprim-resistant S1 DHFR from Staphylococcus aureus?";
RL   J. Bacteriol. 177:2965-2970(1995).
CC   -!- FUNCTION: Not resistant to trimethoprim.
CC   -!- CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-
CC       dihydrofolate + NADPH.
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis;
CC       tetrahydrofolate from dihydrofolate: step 1/1.
CC   -!- MISCELLANEOUS: The reaction catalyzed by this enzyme represents an
CC       essential step for de novo glycine and purine synthesis, DNA
CC       precursor synthesis, and for the conversion of dUMP to dTMP.
CC   -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC   -!- SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.
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DR   EMBL; Z48233; CAA88269.1; -; Genomic_DNA.
DR   PIR; A57271; A57271.
DR   HSSP; P00379; 1DHI.
DR   BRENDA; 1.5.1.3; 874.
DR   GO; GO:0004146; F:dihydrofolate reductase activity; IEA:EC.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR   GO; GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR012259; DHFR.
DR   InterPro; IPR001796; DHFR_reg.
DR   InterPro; IPR017925; Dihydrofolate_reductase_CS.
DR   PANTHER; PTHR11549:SF1; DHFR; 1.
DR   Pfam; PF00186; DHFR_1; 1.
DR   PRINTS; PR00070; DHFR.
DR   PROSITE; PS00075; DHFR_1; 1.
DR   PROSITE; PS51330; DHFR_2; 1.
PE   1: Evidence at protein level;
KW   NADP; One-carbon metabolism; Oxidoreductase.
FT   CHAIN         1    161       Dihydrofolate reductase.
FT                                /FTId=PRO_0000186413.
FT   DOMAIN        2    157       DHFR.
FT   MUTAGEN      32     32       V->I: 3-fold increase of KM for
FT                                dihydrofolate.
FT   MUTAGEN      44     44       G->A: 5-fold increase of KM for NADPH.
FT   MUTAGEN      99     99       F->Y: Trimethoprim resistance.
SQ   SEQUENCE   161 AA;  18417 MW;  CB3167940A387EE0 CRC64;
     MTLSIIVAHD KQRVIGYQNQ LPWHLPNDLK HVKQLTTGNT LVMGRKTFNS IGKPLPNRRN
     VVLTNQASFH HEGVDVINSL DEIKELSGHV FIFGGQTLFE AMIDQVDDMY ITVIDGKFQG
     DTFFPPYTFE NWEVESSVEG QLDEKNTIPH TFLHLVRRKG K
//