Reviewed, UniProtKB/Swiss-Prot P0C0P0 (DYR_STAEP)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase
      Short name=DHFR
    EC=1.5.1.3

Gene names

Name:	folA
Synonyms:	dfrC, folA1

Organism

Staphylococcus epidermidis

Taxonomic identifier

1282 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Not resistant to trimethoprim.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	Dihydrofolate reductase		PRO_0000186413
Regions
	Domain	2 – 157	156	DHFR
Experimental info
	Mutagenesis	32	1	V → I: 3-fold increase of KM for dihydrofolate
	Mutagenesis	44	1	G → A: 5-fold increase of KM for NADPH
	Mutagenesis	99	1	F → Y: Trimethoprim resistance

Sequences

	Sequence		Length	Mass (Da)
	P0C0P0-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: CB3167940A387EE0	FASTA	161	18,417
	10 20 30 40 50 60 MTLSIIVAHD KQRVIGYQNQ LPWHLPNDLK HVKQLTTGNT LVMGRKTFNS IGKPLPNRRN 70 80 90 100 110 120 VVLTNQASFH HEGVDVINSL DEIKELSGHV FIFGGQTLFE AMIDQVDDMY ITVIDGKFQG 130 140 150 160 DTFFPPYTFE NWEVESSVEG QLDEKNTIPH TFLHLVRRKG K

References

[1]

"Characterization of the gene for the chromosomal dihydrofolate reductase (DHFR) of Staphylococcus epidermidis ATCC 14990: the origin of the trimethoprim-resistant S1 DHFR from Staphylococcus aureus?" Dale G.E., Broger C., Hartman P.G., Langen H., Page M.G.P., Then R.L., Stueber D. J. Bacteriol. 177:2965-2970(1995) [PubMed: 7768789] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS. Strain: ATCC 14990 / DSM 20044 / CIP 81.55.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z48233 Genomic DNA. Translation: CAA88269.1.
PIR	A57271.
3D structure databases
HSSP	HSSP built from PDB template 1DHI based on UniProtKB P00379.
ModBase	Search...
Family and domain databases
InterPro	IPR012259. DHFR. IPR001796. DHFR_reg. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_STAEP

Accession

Primary (citable) accession number: P0C0P0
Secondary accession number(s): Q59908

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	November 25, 2008
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)