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Protein

Serine-protein kinase RsbW

Gene

rsbW

Organism
Staphylococcus epidermidis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Negative regulator of sigma-B activity. Phosphorylates and inactivates its specific antagonist protein, RsbV. Upon phosphorylation of RsbV, RsbW is released and binds to sigma-B, thereby blocking its ability to form an RNA polymerase holoenzyme (E-sigma-B).UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine-protein kinase RsbWUniRule annotation (EC:2.7.11.1UniRule annotation)
Alternative name(s):
Anti-sigma-B factorUniRule annotation
Sigma-B negative effector RsbWUniRule annotation
Gene namesi
Name:rsbWUniRule annotation
OrganismiStaphylococcus epidermidis
Taxonomic identifieri1282 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 159159Serine-protein kinase RsbWPRO_0000203545Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi176279.SERP1678.

Structurei

3D structure databases

ProteinModelPortaliP0C0M6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the anti-sigma-factor family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZBE. Bacteria.
COG2172. LUCA.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
HAMAPiMF_00638. Anti_sigma_B.
InterProiIPR003594. HATPase_C.
IPR010193. RsbW.
[Graphical view]
PfamiPF13581. HATPase_c_2. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR01924. rsbW_low_gc. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C0M6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSTQDYIEM RLPASAEYVS LIRLTLSGVF SRAGASYDDI EDSKIAVSEA
60 70 80 90 100
VTNAVKHAYK KNSEIGMINL CFEIFDDRIK IVISDQGESF DYEATKSHLG
110 120 130 140 150
PYNDNENIDF LREGGLGLFL IESLMDEVTV YKESGVTISM IKYIKKEQVR

NNGERVEIS
Length:159
Mass (Da):17,922
Last modified:November 22, 2005 - v1
Checksum:iEA6A49C76218B78F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti81 – 811I → V in AAL37942 (PubMed:11705980).Curated
Sequence conflicti94 – 941A → S in AAL37942 (PubMed:11705980).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF274004 Genomic DNA. Translation: AAG23812.1.
AF359562 Genomic DNA. Translation: AAL37942.1.
RefSeqiWP_001829903.1. NZ_LWBW01000093.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF274004 Genomic DNA. Translation: AAG23812.1.
AF359562 Genomic DNA. Translation: AAL37942.1.
RefSeqiWP_001829903.1. NZ_LWBW01000093.1.

3D structure databases

ProteinModelPortaliP0C0M6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176279.SERP1678.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108ZBE. Bacteria.
COG2172. LUCA.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
HAMAPiMF_00638. Anti_sigma_B.
InterProiIPR003594. HATPase_C.
IPR010193. RsbW.
[Graphical view]
PfamiPF13581. HATPase_c_2. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR01924. rsbW_low_gc. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Biofilm formation by Staphylococcus epidermidis depends on functional rsbU, an activator of the sigB operon: differential activation mechanisms due to ethanol and salt stress."
    Knobloch J.K.-M., Bartscht K., Sabottke A., Rohde H., Feucht H.-H., Mack D.
    J. Bacteriol. 183:2624-2633(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Clinical isolate 1457.
  2. "Identification of the sigB operon in Staphylococcus epidermidis: construction and characterization of a sigB deletion mutant."
    Kies S., Otto M., Vuong C., Gotz F.
    Infect. Immun. 69:7933-7936(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiRSBW_STAEP
AccessioniPrimary (citable) accession number: P0C0M6
Secondary accession number(s): Q8VSV5, Q9F7V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: July 6, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.