P0C0M5 (TYSY_STAES) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	SE_1120

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus epidermidis

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Thymidylate synthase HAMAP-Rule MF_00008		PRO_0000141025
Sites
	Active site	201	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C0M5 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 4CE480445CF9653A	FASTA	318	37,178
	10 20 30 40 50 60 MYNPFDEAYH GLCEEILEIG NRRDDRTHTG TISKFGHQLR FDLTKGFPLL TTKKVSFKLV 70 80 90 100 110 120 ATELLWFIKG DTNIQYLLKY NNNIWNEWAF ENYVQSDDYH GPDMTDFGHR SQQDPEFNEQ 130 140 150 160 170 180 YKEEMKKFKE RILNDDAFAK KYGNLGNVYG KQWRDWEDKN GNHYDQLKSV IQQIKTNPNS 190 200 210 220 230 240 RRHIVSAWNP TEIDSMALPP CHTMFQFYVQ EGKLNCQLYQ RSADIFLGVP FNIASYALLT 250 260 270 280 290 300 HLVAKECGLE VGEFIHTFGD AHIYSNHMDA IHTQLSRDSY LPPQLKINTD KSIFDINYED 310 LELINYESHP AIKAPIAV

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO04717.1.
RefSeq	NP_764675.1. NC_004461.1.
3D structure databases
ProteinModelPortal	P0C0M5.
SMR	P0C0M5. Positions 5-318.
ModBase	Search...
Protein-protein interaction databases
STRING	176280.SE1120.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO04717; AAO04717; SE_1120.
GeneID	1057485.
KEGG	sep:SE1120.
PATRIC	19608040. VBIStaEpi113981_1093.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
KO	K00560.
OMA	ANPWNRR.
ProtClustDB	PRK01827.
Enzyme and pathway databases
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 2 hits.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_STAES

Accession

Primary (citable) accession number: P0C0M5
Secondary accession number(s): P0A0M4, P13954, Q59907

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program